Directed evolution of Penicillium janczewskii zalesk α-galactosidase toward enhanced activity and expression in Pichia pastoris

Applied Biochemistry and Biotechnology

Volumn 168, Issue 3, 2012, Pages 638-650

  Chen, Yiqun ^a   Zhang, Bo ^a,b   Pei, Honglei ^a   Lv, Junnan ^a   Yang, Wenhan ^a   Cao, Yunhe ^a   Dong, Bing ^a  

^a CHINA AGRICULTURAL UNIVERSITY   (China)

^b Beijing Research Institute for Nutritional Resources   (China)

Author keywords

Galactosidase; Directed evolution; Improved activity; Pichia pastoris

Indexed keywords

DIRECTED EVOLUTION; DNA SHUFFLING; ERROR PRONE PCR; FEED ADDITIVES; GALACTOSIDASES; MONOGASTRIC ANIMALS; MUTANT ENZYMES; MUTATION SITES; NUTRIENT DIGESTIBILITY; PCR ANALYSIS; PICHIA PASTORIS; SPECIFIC ACTIVITY; WILD TYPES;

COPPER; METAL IONS; METHANOL; POLYMERASE CHAIN REACTION; YEAST;

ENZYME ACTIVITY;

4 NITROPHENYLGALACTOSIDE; ALPHA GALACTOSIDASE; BACTERIAL DNA; CALCIUM ION; COPPER ION; EDETIC ACID; FERROUS ION; GLUCOSE; MAGNESIUM ION; MANGANESE; MUTANT PROTEIN; PEPTONE; POTASSIUM ION; SODIUM ION; ZINC ION;

ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIUM MUTANT; BIOREACTOR; CONTROLLED STUDY; DIGESTION; DNA SHUFFLING; ENZYME ACTIVITY; ENZYME STABILITY; EXPRESSION VECTOR; FERMENTATION; GENE MUTATION; GROWTH CURVE; NONHUMAN; PENICILLIUM; PENICILLIUM JANCZEWSKII ZALESK; PH; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; SCREENING; TEMPERATURE; WILD TYPE; YEAST;

ALPHA-GALACTOSIDASE; DIRECTED MOLECULAR EVOLUTION; ENZYME STABILITY; FUNGAL PROTEINS; GENE EXPRESSION; MUTATION, MISSENSE; PENICILLIUM; PICHIA;

ANIMALIA; PENICILLIUM JANCZEWSKII; PICHIA PASTORIS;

EID: 84869111158     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-012-9806-5     Document Type: Article

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