Expression of an alkalo-tolerant fungal xylanase enhanced by directed evolution in Pichia pastoris and Escherichia coli

Journal of Biotechnology

Volumn 141, Issue 1-2, 2009, Pages 26-30

  Mchunu, Nokuthula Peace ^a   Singh, Suren ^a   Permaul, Kugen ^a  

^a DURBAN UNIVERSITY OF TECHNOLOGY   (South Africa)

Author keywords

Directed evolution; Pichia pastoris; Xylanase; Yeast expression

Indexed keywords

AGAR PLATES; ALKALINE CONDITIONS; CULTURE MEDIUMS; DIRECTED EVOLUTION; E. COLI; ERROR-PRONE PCR; EXTRACELLULAR; HOST PROTEINS; P. PASTORIS; PICHIA PASTORIS; PULP AND PAPER INDUSTRIES; THERMOMYCES LANUGINOSUS; XYLANASE; YEAST EXPRESSION;

ENZYME ACTIVITY; ENZYMES; ESCHERICHIA COLI; POLYSACCHARIDES; YEAST;

PAPER AND PULP INDUSTRY;

AGAR; XYLAN; XYLAN ENDO 1,3 BETA XYLOSIDASE;

ARTICLE; CELL ACTIVITY; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME RELEASE; ESCHERICHIA COLI; EVOLUTION; GENE EXPRESSION; MOLECULAR CLONING; MUTAGENESIS; NONHUMAN; PAPER INDUSTRY; PH; PICHIA PASTORIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION;

ASCOMYCOTA; DIRECTED MOLECULAR EVOLUTION; ENDO-1,4-BETA XYLANASES; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; HYDROGEN-ION CONCENTRATION; MUTAGENESIS; PICHIA; POLYMERASE CHAIN REACTION; TEMPERATURE;

ENZYMATIC ACTIVITY; PAPER INDUSTRY; POLYSACCHARIDES; PULP INDUSTRY; STABILITY; XYLANASE; YEASTS;

ESCHERICHIA COLI; PICHIA PASTORIS; THERMOMYCES LANUGINOSUS;

EID: 64249099161     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2009.02.021     Document Type: Article

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