Directed evolution of a thermophilic endoglucanase (Cel5A) into highly active Cel5A variants with an expanded temperature profile

Journal of Biotechnology

Volumn 154, Issue 1, 2011, Pages 46-53

  Liang, Chaoning ^a,b   Fioroni, Marco ^c   Rodríguez Ropero, Francisco ^c   Xue, Yanfen ^a   Schwaneberg, Ulrich ^c   Ma, Yanhe ^a  

^a INSTITUTE OF MICROBIOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^c RWTH AACHEN UNIVERSITY   (Germany)

Author keywords

Directed evolution; Endoglucanase; Error prone PCR; Molecular Dynamics; Thermoanaerobacter tengcongensis

Indexed keywords

CARBOXYMETHYL CELLULOSE; CATALYTIC ACTIVITY; CONGO RED; DIRECTED EVOLUTION; E. COLI; ENDOGLUCANASE; ENDOGLUCANASES; ERROR-PRONE PCR; EXPRESSION LEVELS; MOLECULAR DYNAMICS SIMULATIONS; OPTIMAL TEMPERATURE; RARE CODON; SCREENING METHODS; SPECIFIC ACTIVITY; TEMPERATURE PROFILES; THERMOANAEROBACTER TENGCONGENSIS; WILD TYPES;

AMINO ACIDS; AZO DYES; ESCHERICHIA COLI; HYDROGEN BONDS; MOLECULAR DYNAMICS;

CATALYST ACTIVITY;

CARBOXYMETHYLCELLULOSE; ENDOGLUCANASE CEL5A; GLUCAN SYNTHASE; ISOLEUCINE; UNCLASSIFIED DRUG; VALINE;

AMINO ACID SEQUENCE; ARTICLE; CODON; DIRECTED MOLECULAR EVOLUTION; ENZYME ACTIVITY; ENZYME STRUCTURE; HYDROGEN BOND; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SEQUENCE ANALYSIS; STRUCTURE ANALYSIS; TEMPERATURE SENSITIVITY; THERMOANAEROBACTER; THERMOANAEROBACTER TENGCONGENSIS;

AMINO ACID SUBSTITUTION; CELLULASE; DIRECTED MOLECULAR EVOLUTION; GENETIC TESTING; KINETICS; MOLECULAR DYNAMICS SIMULATION; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; PROTEIN STRUCTURE, SECONDARY; TEMPERATURE; THERMOANAEROBACTER;

ESCHERICHIA COLI; THERMOANAEROBACTER TENGCONGENSIS;

EID: 79957475689     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2011.03.025     Document Type: Article

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