Development of a diagnostic real-time polymerase chain reaction assay for the detection of invasive Haemophilus influenzae in clinical samples

Diagnostic Microbiology and Infectious Disease

Volumn 74, Issue 4, 2012, Pages 356-362

  Meyler, Kenneth L ^a   Meehan, Mary ^a   Bennett, Desiree ^a   Cunney, Robert ^a,b   Cafferkey, Mary ^a,c  

^a CHILDREN S UNIVERSITY HOSPITAL   (Ireland)

^b HEALTH PROTECTION SURVEILLANCE CENTRE   (Ireland)

^c ROYAL COLLEGE OF SURGEONS IN IRELAND   (Ireland)

Author keywords

BexA; FucK; H. influenzae; Hpd 3; LicA; OmpP2; Real time PCR

Indexed keywords

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM DETECTION; BIOASSAY; BLOOD CULTURE; CEREBROSPINAL FLUID CULTURE; DIAGNOSTIC ACCURACY; DNA SEQUENCE; FUCK GENE; HAEMOPHILUS INFLUENZAE TYPE B; INVASIVE SPECIES; LICA GENE; NONHUMAN; NUCLEOTIDE SEQUENCE; OMPP2 GENE; PRIORITY JOURNAL; PROCESS DEVELOPMENT; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

HAEMOPHILUS INFECTIONS; HAEMOPHILUS INFLUENZAE; HUMANS; MOLECULAR DIAGNOSTIC TECHNIQUES; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 84869089358     PISSN: 07328893     EISSN: 18790070     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2012.08.018     Document Type: Article

References (43)

1. Abdeldaim G.M., Strålin K., Kirsebom L.A., Olcén P., Blomberg J., Herrman B. Detection of Haemophilus influenzae in respiratory secretions from pneumoniae patients by quantitative real-time polymerase chain reaction. Diagn Microbiol Infect Dis 2009, 64:366-373.
2. Abdeldaim G.M., Strålin K., Korsgaard J., Blomberg J., Welinder-Olsson C. Multiplex quantitative PCR for detection of lower respiratory tract infection and meningitis caused by Streptococcus pneumoniae, Haemophilus influenzae and Neisseria meningitidis. BMC Microbiol 2010, 10:310-318.
3. Adam H.J., Richardson S.E., Jamieson F.B., Rawte P., Low D.E., Fisman D.N. Changing epidemiology of invasive Haemophilus influenzae in Ontario, Canada: evidence for herd effects and strain replacement due to Hib vaccination. Vaccine 2010, 28:4073-4078.
4. Al-Soud W.A., Rådström P. Purification and characterization of PCR-inhibitory components in blood cells. J Clin Microbiol 2001, 39:485-493.
5. Al-Soud W.A., Jönsson L.J., Râdström P. Identification and characterization of immunoglobulin G in blood as a major inhibitor of diagnostic PCR. J Clin Microbiol 2000, 38:345-350.
6. Anderson R., Wang X., Briere E.C., Katz L.S., Cohn A.C., Clark T.A., et al. Haemophilus haemolyticus causing clinical disease. J Clin Microbiol 2012, 50:2462-2465.
7. Binks M.J., Temple B., Kirkham L.-A., Wiertsema S.P., Dunne E.M., et al. Molecular surveillance of true nontypeable Haemophilus influenzae: an evaluation of PCR screening assays. PLoS One 2012, 7(3):e34083.
8. Chiba N., Murayama S.Y., Morozumi M., Nakayama E., Okada T., Iwata S., et al. Rapid detection of eight causative pathogens for the diagnosis of bacterial meningitis by real-time PCR. J Infect Chemother 2009, 15:92-98.
9. Corless C.E., Guiver M., Borrow R., Edwards-Jones V., Fox A.J., Kaczmarski E.B. Simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in suspected cases of meningitis and septicemia using real-time PCR. J Clin Microbiol 2001, 39:1553-1558.
10. Dalton H.P., Allison M.J. Modification of laboratory results by partial treatment of bacterial meningitis. Am J Clin Pathol 1968, 49:410-413.
11. Dworkin M.S., Park L., Borchardt S.M. The changing epidemiology of invasive Haemophilus influenzae disease, especially in persons > or = 65 years old. Clin Infect Dis 2007, 44:810-816.
12. Erwin A.L., Smith A.L. Nontypeable Haemophilus influenzae: understanding virulence and commensal behaviour. Trends Microbiol 2007, 15:355-362.
13. Espy M.J., Uhl J.R., Sloan L.M., Buckwalter S.P., Jones M.F., Vetter E.A., et al. Real-time PCR in clinical microbiology: applications for routine laboratory testing. Clin Microbiol Rev 2006, 19:165-256.
14. Foxwell A.R., Kyd J.M., Cripps A.W. Non typeable Haemophilus influenzae: pathogenesis and prevention. Microbiol Mol Biol Rev 1998, 62:294-308.
15. Hall T.A. A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 1999, 41:95-98.
16. Hamed K.A., Dormitzer P.R., Su C.K., Relman D.A. Haemophilus parainfluenzae endocarditis: application of a molecular approach for identification of pathogenic bacterial species. Clin Infect Dis 1994, 19:677-683.
17. Health Protection Surveillance Centre. (2010). Annual Report can be downloaded from http://www.hpsc.ie/hpsc/AboutHPSC/AnnualReports/File,13092,en.pdf.
18. Hedegaard J., Åkkels H., Bruun B., Kilian M., Mortensen K.K., Norskov-Lauritsen N. Phylogeny of the genus Haemophilus as determined by comparison of partial infB sequences. Microbiology 2001, 147:2599-2609.
19. Hobson R.P., Williams A., Rawal K., Pennington T.H., Forbes K.J. Incidence and spread of Haemophilus influenzae on an Antarctic base determined using the polymerase chain reaction. Epidemiol Infect 1995, 114:93-103.
20. Hogg J.S., Hu F.Z., Janto B., Boissy R., Hayes J., Keefe R., et al. Characterisation and modeling of the Haemophilus influenzae supragenomes based on the complete genomic sequences of Rd and 12 clinical nontypeable strains. Genome Biol 2007, 8(6):R103.
21. Kais M., Spindler C., Kalin M., Ortqvist A., Giske C.G. Quantitative detection of Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis in lower respiratory tract samples by real-time PCR. Diagn Microbiol Infect Dis 2006, 55:169-178.
22. Ladhani S., Slack M.P., Heath P.T., von Gottberg A., Chandra M., Ramsay M.E. Invasive Haemophilus influenzae disease, Europe, 1996-2006. Emerg Infect Dis 2010, 16:455-463.
23. Laupland K.B., Schønheyder H.C., Østergaard C., Knudsen J.D., Valiquette L., Galbraith J., et al. International Bacteremia Surveillance Collaborative. Epidemiology of Haemophilus influenzae bacteremia: a multi-national population-based assessment. J Infect 2011, 62:142-148.
24. Marty A., Greiner O., Day P.J., Gunziger S., Muhlemann K., Nadal D. Detection of Haemophilus influenzae type b by real-time PCR. J Clin Microbiol 2004, 42:3813-3815.
25. Maaroufi Y., De Bruyne J.M., Heymans C., Crokaert F. Real-time PCR for determining capsular serotypes of Haemophilus influenzae. J Clin Microbiol 2007, 45:2305-2308.
26. Meats E., Feil E.J., Stringer S., Cody A.J., Goldstein R., Kroll J.S., et al. Characterisation of encapsulated and noncapsulated Haemophilus influenzae and determination of phylogenetic relationships by multilocus sequence typing. J Clin Microbiol 2003, 41:1623-1636.
27. Morozumi M., Nakayama E., Iwata S., Aoki Y., Hasegawa K., Kobayashi R., et al. Simultaneous detection of pathogens in clinical samples from patients with community-acquired pneumonia by real-time PCR with pathogen-specific molecular beacon probes. J Clin Microbiol 2006, 44:1440-1446.
28. Norskov-Lauritsen N., Kilian M. Reclassification of Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus, Haemophilus paraphrophilus and Haemophilus segnis as Aggregatibacter actinomycetemcomitans gen. nov.,comb. nov., Aggregatibacter aphrophilus comb. nov. and Aggregatibacter segnis comb. nov., and emended description of Aggregatibacter aphrophilus to include V factor-dependent and V factor-independent isolates. Int J Syst Evol Microbiol 2006, 56:2135-2146.
29. Nørskov-Lauritsen N., Overballe M.D., Kilian M. Delineation of the species Haemophilus influenzae by phenotype, multilocus sequence phylogeny, and detection of marker genes. J Bacteriol 2009, 191:822-831.
30. Nørskov-Lauritsen N. Detection of cryptic genospecies misidentified as Haemophilus influenzae in routine clinical samples by assessment of marker genes fucK, hap and sod. J Clin Microbiol 2009, 47:2590-2592.
31. Ohkusu K., Nash K.A., Inderlied C.B. Molecular characterisation of Haemophilus influenzae type a and untypeable strains isolated simultaneously from cerebrospinal fluid and blood: novel use of quantitative real-time PCR based on the cap copy number to determine virulence. Clin Microbiol Infect 2005, 11:637-643.
32. Peltola H. Worldwide Haemophilus influenzae type b disease at the beginning of the 21st century: global analysis of the disease burden 25 years after the use of the polysaccharide vaccine and a decade after the advent of conjugates. Clin Microbiol Rev 2000, 13:302-317.
33. Pittman M. Variation and type specificity in the bacterial species Hemophilus influenzae. J Exp Med 1931, 53:471-492.
34. Resman F., Ristovski M., Ahl J., Forsgren A., Gilsdorf J.R., Jasir A., et al. Invasive disease caused by Haemophilus influenzae in Sweden 1997-2009; evidence of increasing incidence and clinical burden of non-type b strains. Clin Microbiol Infect 2011, 17:1638-1645.
35. Ridderberg W., Fenger M.G., Norskov-Lauritsen N. Haemophilus influenzae may be untypeable by the multilocus sequence typing scheme due to a complete deletion of the fucose operon. J Med Microbiol 2010, 59:740-742.
36. Roush S.W., McIntyre L., Baldy L.M. Manual for the surveillance of vaccine-preventable diseases 2008, Centre for Disease Control and Prevention, Atlanta, GA. 4th ed.
37. Shuel M.L., Karlowsky K.E., Law D.K.S., Tsang R.S.W. Nonencapsulated or nontypeable Haemophilus influenzae are more likely than their encapsulated or serotypeable counterparts to have mutations in their fucose operon. Can J Microbiol 2011, 57:982-986.
38. Smith-Vaughan H., Byun R., Nadkarni M., Jacques N.A., Hunter N., Halpin S., et al. Measuring nasal bacterial load and its association with otitis media. BMC Ear Nose Throat Disord 2006, 6:10-19.
39. Trollfors B., Brorson J.E., Claesson B., Sandberg T. Invasive infections caused by Haemophilus species other than Haemophilus influenzae. Infection 1985, 13:12-14.
40. Ulanova M., Tsang R.S. Invasive Haemophilus influenzae disease: changing epidemiology and host-parasite interactions in the 21st century. Infect Genet Evol 2009, 9:594-605.
41. Wang X., Mair R., Hatcher C., Theodore M.J., Edmond K., Wu H.M., et al. Detection of bacterial pathogens in Mongolia meningitis surveillance with a new real-time PCR assay to detect Haemophilus influenzae. Int J Med Microbiol 2011, 301:303-309.
42. Wang X., Theodore M.J., Mair R., Trujillo-Lopez E., du Plessis M., Wolter N., et al. Clinical validation of multiplex real-time PCR assays for detection of bacterial meningitis pathogens. J Clin Microbiol 2012, 50:702-708.
43. Weiser J.N., Shchepetov M., Chong S.T. Decoration of lipopolysaccharide with phosphorylcholine: a phase variable characteristic of Haemophilus influenzae. Infect Immun 1997, 65:943-950.