Identification and partial characterization of extracellular aspartic protease genes from Metschnikowia pulcherrima IWBT Y1123 and candida apicola IWBT Y1384

Applied and Environmental Microbiology

Volumn 78, Issue 19, 2012, Pages 6838-6849

  Reid, Vernita J ^a   Theron, Louwrens W ^a   Toit, Maret Du ^a   Divol, Benoit ^a  

^a STELLENBOSCH UNIVERSITY   (South Africa)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARTIC PROTEASE; AVAILABLE NITROGEN; BOVINE SERUM ALBUMINS; EXTRACELLULAR; EXTRACELLULAR ACIDS; GENE SEQUENCES; GRAPE JUICE; HAZE FORMATION; HETEROLOGOUS EXPRESSION; IN-SILICO; NON-SACCHAROMYCES; PARTIAL CHARACTERIZATION; PLATE ASSAY; PROTEASE ACTIVITIES; PUTATIVE PROTEINS; STRAIN-DEPENDENT; WINE YEAST;

CANDIDA; ENCODING (SYMBOLS); ENZYME ACTIVITY; FRUIT JUICES; GENE ENCODING; PROTEINS;

YEAST;

ASPARTIC PROTEINASE; FUNGAL DNA; PROTEIN;

BIOASSAY; ENZYME ACTIVITY; FERMENTATION; GENE EXPRESSION; GROWTH RATE; HOMOLOGY; IDENTIFICATION METHOD; NITROGEN; PROTEIN; VINE; YEAST;

AMINO ACID SEQUENCE; ARTICLE; CANDIDA; CHEMISTRY; CLUSTER ANALYSIS; CULTURE MEDIUM; DNA SEQUENCE; ENZYMOLOGY; GENE EXPRESSION; GENETICS; HYDROLYSIS; METABOLISM; METSCHNIKOWIA; MOLECULAR GENETICS; PHYLOGENY; SACCHAROMYCES CEREVISIAE; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; ASPARTIC ACID ENDOPEPTIDASES; CANDIDA; CLUSTER ANALYSIS; CULTURE MEDIA; DNA, FUNGAL; GENE EXPRESSION; HYDROLYSIS; METSCHNIKOWIA; MOLECULAR SEQUENCE DATA; PHYLOGENY; PROTEINS; SACCHAROMYCES CEREVISIAE; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID;

BOVINAE; CANDIDA APICOLA; METSCHNIKOWIA PULCHERRIMA; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE; VITACEAE;

EID: 84868307814     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00505-12     Document Type: Article

References (53)

1. Albrecht A, et al. 2006. Glycosylphosphatidylinositol-anchored proteases of Candida albicans target proteins necessary for both cellular processes and host-pathogen interactions. J. Biol. Chem. 281:688-694.
2. Banerjee A, Ganesan K, Datta A. 1991. Induction of secretory acid proteinase in Candida albicans. J. Gen. Microbiol. 137:2455-2461.
3. Barrett AJ, Rawlings ND, O'Brien EA. 2001. The MEROPS database as a protease information system. J. Struct. Biol. 134:95-102.
4. Beckerich J-M, Boisramé-Baudevin A, Gaillardin C. 1998. Yarrowia lipolytica: a model organism for protein secretion studies. Int. Microbiol. 1:123-130.
5. Bossi A, Bonizzato L, Zapparoli G. 2006. Acidic extracellular proteases from microrganisms of fairly acidic niche. Protein. Pept. Lett. 13:737-741.
6. Chambers A, et al. 1989. Transcriptional control of the Saccharomyces cerevisiae PGK gene by RAPI. Mol. Cell. Biol. 9:5516-5524.
7. Charoenchai C, Fleet GH, Henschke PA, Todd BENT. 1997. Screening of non-Saccharomyces wine yeasts for the presence of extracellular hydrolytic enzymes. Aust. J. Grape Wine Res. 3:2-8.
8. Ciani M, Comitini F. 2011. Non-Saccharomyces wine yeasts have a promising role in biotechnological approaches to winemaking. Ann. Microbiol. 61:25-32.
9. Ciani M, Maccarelli F. 1998. Oenological properties of non-Saccharomyces yeasts associated with wine-making. World J. Microbiol. Biotechnol. 14:199-203.
10. Collart MA, Oliviero S. 1993. Preparation of yeast RNA, p 13.12.1-13.12.5. In Ausubel FM, et al (ed), Current protocols in molecular biology, vol 2.13.1. John Wiley, New York, NY.
11. Dabas N, Morschhäuser J. 2008. A transcription factor regulatory cascade controls secreted aspartic protease expression in Candida albicans. Mol. Microbiol. 69:586-602.
12. Davies DR. 1990. The structure and function of aspartic proteases. Annu. Rev. Biophys. Biophys. Chern. 19:189-215.
13. Dujon B, et al. 2004. Genome evolution in yeasts. Nature 430:35-44.
14. Dunn BM. 2002. Structure and mechanism of the pepsin-like family of aspartic peptidases. Chem. Rev. 102:4431-4458.
15. Fleet GH. 2003. Yeast interactions and wine flavour. Int. J. Food Microbiol. 86:11-22.
16. Folio P, Ritt J, Alexandre H, Remize F. 2008. Characterization of EprA, a major extracellular protein of Oenococcus oeni with protease activity. Int. J. Food Microbiol. 127:26-31.
17. Gotoh T, et al. 1995. Purification and properties of extracellular carboxyl proteinase secreted by Candida pulcherrima. Biosci. Biotechnol. Biochem. 59:367-371.
18. Gundllapalli S, Cordero Otero R, Pretorius IS. 2006. Development of a screening method for the identification of a novel Saccharomyces cerevisiae mutant over-expressing Trichoderma reesei cellobiohydrolase II. Ann. Microbiol. 56:143-150.
19. Hoffman CS, Winston F. 1987. A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli. Gene 57:267-272.
20. Jolly NP, Augustyn OPH, Pretorius IS. 2003. The use of Candida pulcherrima in combination with Saccharomyces cerevisiae for the production of Chenin blanc wine. S. Afr. J. Enol. Vitic. 24:63-69.
21. Jolly NP, Augustyn OPH, Pretorius IS. 2006. The role and use of non-Saccharomyces yeasts in wine production. S. Afr. J. Enol. Vitic. 27:15-39.
22. Koszul R, et al. 2003. The complete mitochondrial genome sequence of the pathogenic yeast Candida (Torulopsis) glabrata. FEBS Lett. 534:39-48.
23. Kurtzman CP, Robnett CJ. 1998. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie Van Leeuwenhoek 73:331-371.
24. Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 277:680-685.
25. Lagace LS, Bisson LF. 1990. Survey of yeast acid proteases for effectiveness of wine haze reduction. Am. J. Enol. Vitic. 41:147-155.
26. Lambrechts MG, Pretorius IS. 2000. Yeast and its importance to wine aroma. S. Afr. J. Enol. Vitic. 21:97-129.
27. Le Bourse D, et al. 2011. Quantification of chitinase and thaumatin-like proteins in grape juices and wines. Anal. Bioanal. Chem. 401:1541-1549.
28. Li J, et al. 2009. Cloning and characterization of a novel aspartic protease gene from marine-derived Metschnikowia reukaufii and its expression in E. coli. Appl. Biochem. Biotechnol. 159:119-132.
29. Lott TJ, et al. 1998. Sequence analysis of the internal transcribed spacer 2 (ITS2) from yeast species within the genus Candida. Curr. Microbiol. 36:63-69.
30. Marangon M, Van Sluyter SC, Haynes PA, Waters EJ. 2009. Grape and wine proteins: their fractionation by hydrophobic interaction chromatography and identification by chromatographic and proteomic analysis. J. Agric. Food Chem. 57:4415-4425.
31. Marzluf GA. 1997. Genetic regulation of nitrogen metabolism in the fungi. Microbiol. Mol. Biol. Rev. 61:17-32.
32. McEwen RK, Young TW. 1998. Secretion and pH-dependent selfprocessing of the pro-form of the Yarrowia lipolytica acid extracellular protease. Yeast 14:1115-1125.
33. Mellor J, Dobson MJ, Kingsman AJ, Kingsman SM. 1987. A transcriptional activator is located in the coding region of the yeast PGK gene. Nucleic Acids Res. 15:6243-6259.
34. Mendoza L, Farías ME. 2010. Improvement of wine organoleptic characteristics by non-Saccharomyces yeasts. Curr. Res. Technol. Ed. Top. Appl. Microbiol. Microb. Biotechnol. 2:908-919.
35. Moore PA, Sagliocco FA, Wood RMC, Brown AJP. 1991. Yeast glycolytic mRNAs are differentially regulated. Mol. Cell. Biol. 11:5330-5337.
36. Morschhäuser J. 2011. Nitrogen regulation of morphogenesis and protease secretion in Candida albicans. Int. J. Med. Microbiol. 301:390-394.
37. Naglik J, Albrecht A, Bader O, Hube B. 2004. Candida albicans proteinases and host/pathogen interactions. Cell. Microbiol. 6:915-926.
38. Newport G, Agabian N. 1997. KEX2 influences Candida albicans proteinase secretion and hyphal formation. J. Biol. Chem. 272:28954-28961.
39. Ogrydziak DM. 1993. Yeast extracellular proteases. Crit. Rev. Biotechnol. 13:1-55.
40. Ormrod IHL, Lalor EF, Sharpe FR. 1991. The release of yeast proteolytic enzymes into beer. J. Inst. Brew. 97:441-443.
41. Pocock KF, Høj PB, Adams KS, Kwiatkowski MJ, Waters EJ. 2003. Combined heat and proteolytic enzyme treatment of white wines reduces haze forming protein content without detrimental effect. Aust. J. Grape Wine Res. 9:56-63.
42. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV. 1998. Molecular and biotechnological aspects of microbial proteases. Microbiol. Mol. Biol. Rev. 62:597-635.
43. Rodríguez ME, Lopes CA, Barbagelata RJ, Barda NB, Caballero AC. 2010. Influence of Candida pulcherrima Patagonian strain on alcoholic fermentation behaviour and wine aroma. Int. J. Food Microbiol. 138: 19-25.
44. Strauss MLA, Jolly NPNP, Lambrechts MG, Van Rensburg P. 2001. Screening for the production of extracellular hydrolytic enzymes by non-Saccharomyces wine yeasts. J. Appl. Microbiol. 91:182-190.
45. Togni G, Sanglard D, Quadroni M, Foundling SI, Monod M. 1996. Acid proteinase secreted by Candida tropicalis: functional analysis of preproregion cleavages in C. tropicalis and Saccharomyces cerevisiae. Microbiology 142:493-503.
46. Tyndall JDA, Nall T, Fairlie DP. 2005. Proteases universally recognize β-strands in their active sites. Chem. Rev. 105:973-1000.
47. Van Dyk D, Pretorius IS, Bauer FF. 2005. Mss11p is a central element of the regulatory network that controls FLO11 expression and invasive growth in Saccharomyces cerevisiae. Genetics 169:91-106.
48. Volschenk H, et al. 2004. Genetic engineering of an industrial strain of Saccharomyces cerevisiae for L-malic acid degradation via an efficient malo-ethanolic pathway. S. Afr. J. Enol. Vitic. 25:63-73.
49. Waters EJ, Wallace W, Williams PJ. 1992. Identification of heat-unstable wine proteins and their resistance to peptidases. J. Agric. Food Chem. 40:1514-1519.
50. Waters EJ, Shirley NJ, Williams PJ. 1996. Nuisance proteins of wine are grape pathogenesis-related proteins. J. Agric. Food Chem. 44:3-5.
51. Waters EJ, et al. 2005. Preventing protein haze in bottled white wine. Aust. J. Grape Wine Res. 11:215-225.
52. White TJ, Bruns T, Lee S, Taylor J. 1990. Amplification and direct sequencing of fungi ribosomalRNAgenes for phylogenetics, p 315-322. In Innis MA, Gelfand DH, Sninsky JJ, White TJ. (ed), PCR protocols. A guide to methods and applications. Academic Press, San Diego, CA.
53. Young TW, et al. 1996. The extracellular acid protease gene of Yarrowia lipolytica: sequence and pH-regulated transcription. Microbiology 142: 2913-2921.