Gene construction, expression and functional testing of an inotropic peptide from the venom of the black scorpion Hottentotta judaicus

Toxicon

Volumn 60, Issue 8, 2012, Pages 1415-1427

  Tekook, M A ^a   Fabritz, L ^a,b   Kirchhof, P ^a,b   Konig S ^c   Muller F U ^a   Schmitz, W ^a   Tal, T ^d   Zlotkin, E ^d   Kirchhefer, U ^a  

^a UNIVERSITY HOSPITAL MÜNSTER   (Germany)

^b UNIVERSITY OF BIRMINGHAM   (United Kingdom)

^c UNIVERSITY OF MÜNSTER   (Germany)

^d HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

NaScTx; Cardiomyocyte contraction; HPLC; In vitro refolding; PCR; Positive inotropy; Prokaryotic expression; SOE

Indexed keywords

AGAROSE; BJ IP PEPTIDE; ESCHERICHIA COLI PROTEIN; GLUTATHIONE; GLYCEROL; GUANIDINE; HYBRID PROTEIN; INOTROPIC PEPTIDE; PEPTIDE; POLYPEPTIDE; PROTEIN KRX; RECOMBINANT PEPTIDE; RECOMBINANT PROTEIN; SCORPION VENOM; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BIOLOGICAL ACTIVITY; CELL KINETICS; CLASSIFICATION; CODON; CONTROLLED STUDY; DNA FRAGMENTATION; DNA SEQUENCE; ELUTION; ESCHERICHIA COLI; EXPRESSION VECTOR; FUNCTIONAL ASSESSMENT; GENE; GENE CONSTRUCT; GENE EXPRESSION SYSTEM; GENE FUNCTION; GENE SYNTHESIS; HEART MUSCLE CELL; HEART MUSCLE CONTRACTILITY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HOTTENTOTTA JUDAICUS; LONG CHAIN SCORPION TOXIN GENE; MASS SPECTROMETRY; MOLECULAR CLONING; MOUSE; NONHUMAN; OXIDATION REDUCTION REACTION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN ISOLATION; PROTEIN PURIFICATION; REPRODUCIBILITY; SCORPION;

ANIMALS; BASE SEQUENCE; BLOTTING, WESTERN; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CLONING, MOLECULAR; DNA; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; MASS SPECTROMETRY; MUTAGENESIS; PEPTIDES; POLYMERASE CHAIN REACTION; SCORPION VENOMS; SCORPIONS; SEQUENCE HOMOLOGY, NUCLEIC ACID;

ANDROCTONUS CRASSICAUDA; ESCHERICHIA COLI; HEXAPODA; HOTTENTOTTA JUDAICUS; PROKARYOTA; SCORPIONES;

EID: 84868247316     PISSN: 00410101     EISSN: 18793150     Source Type: Journal    
DOI: 10.1016/j.toxicon.2012.10.008     Document Type: Article

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