Expression, renaturation and functional analysis of an excitatory insect-specific toxin from scorpion Buthus martensii Karsch

Protein and Peptide Letters

Volumn 12, Issue 7, 2005, Pages 635-638

  Li, Chong ^a   Liu, Wei ^a   Bossmans, Frank ^b   Zhu, Rong Huan ^a   Tytgat, Jan ^b   Wang, Da Cheng ^a  

^a INSTITUTE OF BIOPHYSICS   (China)

^b UNIVERSITY OF LEUVEN   (Belgium)

Author keywords

BmK IT AP; Denaturation and renaturation; E. coli; Recombinant expression

Indexed keywords

RECOMBINANT PROTEIN; SCORPION VENOM; SODIUM CHANNEL;

ANIMAL; ARTICLE; BIOSYNTHESIS; CHEMISTRY; DRUG EFFECT; ELECTROPHYSIOLOGY; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; LARVA; METABOLISM; MOTH; OOCYTE; PATCH CLAMP; PROTEIN RENATURATION; SCORPION; XENOPUS LAEVIS;

ANIMALS; ELECTROPHYSIOLOGY; GENE EXPRESSION; LARVA; MOTHS; OOCYTES; PATCH-CLAMP TECHNIQUES; PROTEIN RENATURATION; RECOMBINANT PROTEINS; SCORPION VENOMS; SCORPIONS; SODIUM CHANNELS; XENOPUS LAEVIS;

ESCHERICHIA COLI; INSECTA; MAMMALIA; MESOBUTHUS MARTENSII; SCORPIONES;

EID: 24944523754     PISSN: 09298665     EISSN: None     Source Type: Journal    
DOI: 10.2174/0929866054696172     Document Type: Article

References (26)

1. Gordon, D., Savarin, P., Gurevitz, M. and Zinn-Justin, S. (1998) J. Toxicol. Toxin Rev., 17, 131-159.
2. Possani, L.D., Becerril, B., Delepierre, M. and Tytgat, J. (1999) Eur. J. Biochem., 264, 287-300.
3. Couraud, F., Jover, E., Dubois, J.M. and Rochat, H. (1982) Toxicon, 20, 9-16.
4. Gordon, D., Moskowitz, H., Eitan, M., Warner, C., Catterall, W.A. and Zlotkin, E. (1992) Biochemistry, 31, 7622-7628.
5. Zlotkin, E., Rochat, H., Kopeyan, Miranda, F. and Lissitzky, S. (1971) Biochimie, 53, 1073-1078.
6. Pelhate, M. and Zlotkin, E. (1982) J. Exp. Biol, 97, 67-77.
7. Zlotkin, E., Kadouri, D., Gordon, D., Pelhate, M., Martin, M.F. and Rochat, H. (1985) Arch. Biochem. Biophys, 240, 877-887.
8. Stewart, L.M., Hirst, M., Lopez, F.M., Merryweather, A.T., Cayley, P.J. and Possee, R.D. (1991) Nature, 352, 85-88.
9. McCutchen, B.F., Choudary, P.V., Crenshaw, R., Maddox, D., Kamita, S.G., Palekar, N., Volrath, S., Fowler, E., Hammock, B.D. and Maeda, S. (1991) Biotechnology (N. Y.), 9, 848-852.
10. Xiong, Y.M., Lan, Z.D., Wang, M., Liu, B., Liu, X.Q., Fei, H., Xu, L.G., Xia, Q.C., Wang, C.G., Wang, D.C. and Chi, C.W. (1999) Toxicon, 37, 1165-1180.
11. Liu, Z., Yang, G., Li, B., Chi, C. and Wu, X. (2003) Mol. Biotechnol., 24, 21-26.
12. Peng, F., Zeng, X.C., He, X.H., Pu, J., Li, W.X., Zhu, Z.H. and Liu, H. (2002) Eur. J. Biochem., 269, 4468-4475.
13. Zhang, J.H., Hua, Z.C., Xu, Z., Zheng, W.J. and Zhu, D.X. (2001) Prep. Biochem. Biotechnol., 31, 49-57.
14. Johnson, T.M., Quick, M.W., Sakai, T.T. and Krishna, N.R. (2000) Peptides, 21, 767-772.
15. Shao, F., Xiong, Y.M., Zhu, R.H., Ling, M.H., Chi, C.W. and Wang, D.C. (1999) Protein Expr. Purif., 17, 358-365.
16. Turkov, M., Rashi, S., Noam, Z., Gordon, D., Ben Khalifa, R., Stankiewicz, M., Pelhate, M. and Gurevitz, M. (1997) Protein Expr. Purif., 10, 123-131.
17. Zilberberg, N., Gordon, D., Pelhate, M., Adams, M.E., Norris, T.M., Zlotkin, E. and Gurevitz, M. (1996) Biochemistry, 35, 10215-10222.
18. Martin-Eauclaire, M.F., Sogaard, M., Ramos, C., Cestele, S., Bougis, P.E. and Svensson, B. (1994) Eur. J. Biochem., 223, 637-645.
19. Bougis, P.E., Rochat, H. and Smith, L.A. (1989) J. Biol. Chem., 264, 19259-19265.
20. Cohen, L., Karbat, I., Gilles, N., Froy, O., Corzo, G., Angelovici, R., Gordon, D. and Gurevitz, M. (2004) J. Biol. Chem., 279, 8206-8211
21. Schagger, H. and von Jagow, G. (1987) Anal. Biochem., 166, 368-379.
22. Reed, L.J. and Muench, S. (1938) Am. J. Hyg., 27, 493.
23. Noda, M., Ikeda, T., Kayano, T., Suzuki, H., Takeshima, H., Kurasaki, M., Takahashi, H. and Numa, S. (1986) Nature, 320, 188-192.
24. Gellens, M.E., George, A.L. Jr., Chen, L.Q., Chahine, M., Horn, R., Barchi, R.L. and Kallen, R.G. (1992) Proc. Natl. Acad. Sci. USA, 89, 554-558.
25. Warmke, J.W., Reenan, R.A., Wang, P., Qian, S., Arena, J.P., Wang, J., Wunderler, D., Liu, K., Kaczorowski, G.J., Van der Ploeg, L.H., Ganetzky, B. and Cohen, C.J. (1997) J. Gen. Physiol., 110, 119-133.
26. Liu, Y.F., Ma, R.L., Wang, S.L., Duan, Z.Y., Zhang, J.H., Wu, L.J. and Wu, C.F. (2003) Protein Expr. Purif., 27, 253-258.