Heterologous expression of a gene that codes for Pg8, a scorpion toxin of Parabuthus granulatus, capable of generating protecting antibodies in mice

Toxicon

Volumn 53, Issue 7-8, 2009, Pages 770-778

  García Gómez, Blanca I ^a,b   Olamendi Portugal, Timoteo C ^a   Paniagua, Jorge ^b,c   van der Walt, Jurg ^d   Dyason, Karin ^d   Possani, Lourival D ^a  

^a INSTITUTO DE BIOTECNOLOGÍA   (Mexico)

^b Instituto Bioclon SA de CV   (Mexico)

^c Laboratorios Silanes SA de CV   (Mexico)

^d NORTH WEST UNIVERSITY   (South Africa)

Author keywords

Antibody; Cloning; Folding; Gene expression; Parabuthus granulatus; Scorpion toxin

Indexed keywords

AMINO ACID; COMPLEMENTARY DNA; HISTONE; HYBRID PROTEIN; PARABUTHUS GRANULATUS PEPTIDE NUMBER 8; SCORPION VENOM; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANTIBODY PRODUCTION; ARTICLE; BACTERIAL STRAIN; CODON; CONTROLLED STUDY; DNA LIBRARY; ESCHERICHIA COLI; FEMALE; GENE SEQUENCE; HETEROLOGOUS EXPRESSION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYBRID GENE; INSECT GENETICS; LD 50; MOLECULAR CLONING; MOLECULAR RECOGNITION; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PARABUTHUS GRANULATUS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DETERMINATION; PROTEIN PURIFICATION; SCORPION; SEPARATION TECHNIQUE; TOXIN ANALYSIS; TOXIN STRUCTURE;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES; ANTIVENINS; CLONING, MOLECULAR; DNA, COMPLEMENTARY; FEMALE; GENES; MICE; MOLECULAR SEQUENCE DATA; PLASMIDS; RECOMBINANT PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SCORPION VENOMS; SCORPIONS;

MUS; PARABUTHUS GRANULATUS; SCORPIONES;

EID: 67349141338     PISSN: 00410101     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.toxicon.2009.02.011     Document Type: Article

References (23)

1. Bergman N.J. Clinical description of Parabuthus transvaalicus scorpionism in Zimbabwe. Toxicon 35 (1997) 759-771
2. Bouhaouala-Zahar B., Ducancel F., Zenouaki I., Ben Khalifa R., Borchani L., Pelhate M., Boulain J.C., El Ayeb M., Menez, and Karoui A. A recombinant insect specific alpha-toxin of Buthus occitanus tunetanus scorpion confers protection against homologous mammal toxins. Eur. J. Biochem. 238 (1996) 653-660
3. Calderón-Aranda E.S., Dehesa-Dávila M., Chávez-Haro A., and Possani L.D. Scorpion stings and their treatment in México. In: Bon C., and Goyffon M. (Eds). Envenomings and their Treatments (1996), Editions Fondation Marcel Mérieux, Lyon, France 311-320
4. Chuang R.S.I., Jaffe H., Cribbs L., Perez-Reyes E., and Swartz K.J. Inhibition of T-type voltage-gated calcium channels by a new scorpion toxin. Nat. Neurosci. 1 (1998) 668-674
5. Du Plessis L.H., Elgar D., and Du Plessis J.L. Southern African scorpion toxins: an overview. Toxicon 51 (2008) 1-9
6. Dyason K., Brandt W., Prendini L., Verdonck L.F., Tytgat L., Du Plessis J., Müller G., and Van der Walt J. Determination of species-specific components in the venom of Parabuthus scorpions from southern Africa using matrix-assisted laser desorption time-of-flight mass spectrometry. Rapid Commun. Mass Spectrom. 16 (2002) 768-773
7. Estrada G., Garcia B.I., Schiavon E., Ortiz E., Sestéele S., Wanke E., Possani L.D., and Corzo G. Four disulfide-bridged scorpion neurotoxin: heterologous expression and proper folding in vitro. Biophysica Biochemica Acta 1770 (2007) 1161-1168
8. Garcia C., Calderon-Aranda E.S., Anguiano G.A., Becerril B., and Possani L.D. Analysis of the immune response induced by a scorpion venom sub-fraction, a pure peptide and a recombinant peptide, against toxin Cn2 of Centruroides noxius Hoffmann. Toxicon 41 (2003) 417-427
9. Guatimosim S.C.F., Kalapothakis, Diniz E., and Chavez-Olortegui C.R. Induction of neutralizing antibodies against Tityus serrulatus toxins by immunization with a recombinant non toxic protein. Toxicon 38 (2000) 113-121
10. Huys I., Olamendi-Portugal T., Garcia-Gomez B.I., Vandenberghe I., Van Beeumen J., Dyason K., Clynen E., Zhu S., Van der Walt J., Possani L.D., and Tytgat J. A subfamily of acidic alpha-K(+) toxins. J. Biol. Chem. 279 (2004) 2781-2789
11. Inceoglu B., Lango J., Rabinovich A., Whetstone P., and Hammock B.D. The neutralizing effect of a polyclonal antibody raised against the N-terminal eighteen-aminoacid residues of birtoxin towards the whole venom of Parabuthus transvaalicus. Toxicon 47 (2006) 144-149
12. Legros C., Kaabi H., El Ayeb M., Ceard B., Vacher H., Bougis P.E., and Martin-Eauclaire M.F. Use of fusion protein constructs to generate potent immunotherapy and protection against scorpion toxins. Vaccine 20 (2001) 934-942
13. 2+ channels in spermatogenic cells inhibit the sperm acrosome reaction. Biochem. Biophys. Res. Commun. 300 (2003) 408-414
14. Makrides S.C. Strategies for achieving high-level expression of genes in Escherichia coli. Microbiological Reviews 60 (1996) 512-538
15. Müller G.J. Scorpionism in South Africa: a report of 42 serious scorpion envenomations. South Afr. Med. J. 83 (1993) 405-411
16. + channels. Biochem. Biophys. Res. Commun. 299 (2002) 562-568
17. Possani L.D., and Rodríguez de la Vega R.C. Scorpion venom peptides. In: Kastin A.J. (Ed). The Handook of Biologically Active Peptides vol. 51 (2006), Academic Press, San Diego 339-354
18. Prendini L. The systematics of southern African Parabuthus Pocock (scorpiones, buthidae): revisions to the taxonomy and key to the species. The Journal of Arachnology 32 (2002) 109-186
19. Prodromou C., and Peral L.H. Recursive PCR: a novel technique for total gene synthesis. Protein Engineering Design and Selection 5 (1992) 827-829
20. Saravia P., Rojas E., Escalante T., Arce V., Chaves E., Velásquez R., Lomonte B., Rojas G., and Gutiérrez J.M. The venom of Bothrops asper from Guatemala: toxic activities and neutralization by antivenoms. Toxicon 39 (2001) 401-405
21. Schägger H., and Von Jagow G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Analytical Biochemistry 166 (1987) 368-379
22. Sørensen H.P., and Mortensen K.K. Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli. Microbial Cell Factories 4 (2005) 1
23. Turkov M., Rashi S., Noam Z., Gordon D., Califa R.B., Stankiewicz M., Pélhate M., and Gurevitz M. In vitro folding and functional analysis of an anti-insect selective scorpion depressant neurotoxin produced in Escherichia coli. Protein Expression and Purification 10 (1997) 123-131