Cytoprotective effects of fruit pulp of Eugenia jambolana on H 2 O 2-induced oxidative stress and apoptosis in rat Leydig cells in vitro

Andrologia

Volumn 45, Issue 3, 2013, Pages 145-157

  Anand, H ^a,b   Misro, M M ^a   Sharma, S B ^c   Prakash, S ^b  

^a NATIONAL INSTITUTE OF HEALTH AND FAMILY WELFARE   (India)

^b DAYALBAGH EDUCATIONAL INSTITUTE   (India)

^c UNIVERSITY COLLEGE OF MEDICAL SCIENCES   (India)

Author keywords

Apoptosis; Eugenia jambolana; H 2 O 2; Leydig cells; Oxidative stress

Indexed keywords

ACETYLCYSTEINE; CASPASE 3; CASPASE 8; CASPASE 9; CATALASE; CYCLOOXYGENASE 2; FAS ANTIGEN; FAS LIGAND; FLICE INHIBITORY PROTEIN; GLUTATHIONE; GLUTATHIONE TRANSFERASE; HYDROGEN PEROXIDE; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; INDUCIBLE NITRIC OXIDE SYNTHASE; NICOTINAMIDE ADENINE DINUCLEOTIDE ADENOSINE DIPHOSPHATE RIBOSYLTRANSFERASE; NITRIC OXIDE; PROTEIN BCL 2; PROTEIN C FOS; PROTEIN KINASE B; PROTEIN P53; STRESS ACTIVATED PROTEIN KINASE; SUPEROXIDE DISMUTASE; SYNAPTOPHYSIN; SYZYGIUM CUMINI EXTRACT; THIOBARBITURIC ACID REACTIVE SUBSTANCE;

ANIMAL EXPERIMENT; APOPTOSIS; ARTICLE; CELL PROTECTION; CELL SURVIVAL; CONTROLLED STUDY; ENZYME ACTIVITY; FRUIT; IN VITRO STUDY; LEYDIG CELL; MALE; NONHUMAN; OXIDATIVE STRESS; PROTEIN EXPRESSION; RAT; SYZYGIUM CUMINI;

ANIMALS; ANTIOXIDANTS; APOPTOSIS; CASPASES; CATALASE; CELL SURVIVAL; CYTOPROTECTION; EUGENIA; GLUTATHIONE; HYDROGEN PEROXIDE; JNK MITOGEN-ACTIVATED PROTEIN KINASES; LEYDIG CELLS; LIPID PEROXIDATION; MALE; NITRIC OXIDE; OXIDANTS; OXIDATIVE STRESS; PLANT EXTRACTS; RATS; RATS, SPRAGUE-DAWLEY; RNA, MESSENGER; SUPEROXIDE DISMUTASE;

EID: 84868143295     PISSN: 03034569     EISSN: 14390272     Source Type: Journal    
DOI: 10.1111/j.1439-0272.2012.01323.x     Document Type: Article

References (56)

1. Abdelwahab SI, Mohan S, Elhassan MM, Al-Mekhlafi N, Mariod AA, Abdul AB, Abdulla MA, Alkharfy KM (2011) Antiapoptotic and antioxidant properties of Orthosiphon stamineus Benth (Cat's Whiskers): intervention in the Bcl-2-mediated apoptotic pathway. Evid Based Complement Alternat Med 2011:1-11.
2. Aebi H (1984) Catalase in vitro . Meth Enzymol 105:121-126.
3. Aggarwal A, Misro MM, Maheshwari A, Sehgal N, Nandan D (2009) Adverse effects associated with persistent stimulation of Leydig cells with hCG in vitro . Mol Reprod Dev 76:1076-1083.
4. Aggarwal A, Misro MM, Maheshwari A, Sehgal N, Nandan D (2010) N-acetylcysteine counteracts oxidative stress and prevents hCG-induced apoptosis in rat Leydig cells through down regulation of caspase-8 and JNK. Mol Reprod Dev 77:900-909.
5. Allen JA, Diemer T, Janus P, Hales KH, Hales DB (2004) Bacterial endotoxin lipopolysaccharide and reactive oxygen species inhibit Leydig cell steroidogenesis via perturbation of mitochondria. Endocrine 25:265-275.
6. Anakwe OO, Moger WH (1986) Catecholamine stimulation of androgen production by rat Leydig cells, Interactions with luteinizing hormone and luteinizing hormone-releasing hormone. Biol Reprod 35:806-814.
7. Anderson AM, Jorgensen N, Frydelund-Larsen L, Rajpert-De Meyts E, Skakkebaek NE (2004) Impaired Leydig cell function in infertile men: a study of 357 idiopathic infertile men and 318 proven fertile controls. J Clin Endocrinol Metab 89:3161-3167.
8. Arnon J, Meirow D, Lewis-Roness H, Ornoy A (2001) Genetic and teratogenic effects of cancer treatments on gametes and embryos. Hum Reprod Update 7:394-403.
9. Ballester J, Dominguez Carmenr Muoz M, Sensat M, Rigau T, Guinovart JJ, Rodriguez-Gil JE (2005) Tungstate treatment improves Leydig cell function in streptozotocin-diabetic rats. J Androl 26:706-715.
10. Barbouti A, Doulias PT, Nousis L, Tenopoulou M, Galaris D (2002) DNA damage and apoptosis in hydrogen peroxide-exposed jurkat cells: bolus versus continuous generation of H2O2. Free Radic Biol Med 33:691-702.
11. Bhor VM, Raghuram N, Sivakami S (2004) Oxidative damage and altered antioxidant enzyme activities in the small intestine of streptozotocin-induced diabetic rats. Int J Biochem Cell Biol 36:89-97.
12. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 7:248-254.
13. Cao L, Leers-Sucheta S, Azhar S (2004) Aging alters the functional expression of enzymatic and non-enzymatic anti-oxidant defense systems in testicular rat Leydig cells. J Steroid Biochem Mol Biol 88:61-67.
14. Chang MS, Kim WN, Yang WM, Kim HY, Oh JH, Park SK (2008) Cytoprotective effects of Morinda officinalis against hydrogen peroxide-induced oxidative stress in Leydig TM3 cells. Asian J Androl 10:667-674.
15. Chaturvedi A, Kumar MM, Bhawani G, Chaturvedi H, Kumar M, Goel RK (2007) Effect of ethanolic extract of Eugenia jambolana seeds on gastric ulceration and secretion in rats. Ind J Physiol Pharmacol 51:131-140.
16. Das K, Samanta L, Chainy GBN (2000) A modified spectrophotometric assay of superoxide dismutase using nitrite formation of superoxide radicals. Ind J Biochem Biophys 237:201-204.
17. Diemer T, Hales KH, Ginde S, Diemer T, Hales KH, Ginde S, Choi J, Nardulli B, Bosmann HB, Hales DB (2000) Immune activation via injection of Lipopolysaccharide (LPS) in mice results in disruption of Leydig cells steroidogenesis due to oxidative mitochondrial damage. Biol Reprod 63:343-348.
18. El-Beshbishy HA, Tork OM, El-Bab MF, Autifi MA (2011) Antioxidant and antiapoptotic effects of green tea polyphenols against azathioprine-induced liver injury in rats. Pathophysiology 18:125-135.
19. 2 at physiological concentrations modulates Leydig cell function inducing oxidative stress and apoptosis. Apoptosis 11:39-46.
20. Habig WH, Pabst MJ, Jakoby WB (1974) Glutathione-s-transferase. J Biol Chem 249:7130-7139.
21. Han X, Tu Z, Gong Y, Shen S, Wang X, Kang L, Hou Y, Chan J (2004) The toxic effects of nonylphenol on the reproductive system of male rats. Reprod Toxicol 19:215-221.
22. Hierholzer C, Harbecht B, Menezes JM, Kane J, MacMicking J, Nathan CF, Peitzman AB, Billiar TR, Tweardy DJ (1998) Essential role of induced nitric oxide in the initiation of the inflammatory response after hemorrhagic shock. J Exp Med 187:917-928.
23. Joensen UN, Jorgensen N, Rajpert-De Meyts E, Skakkebaek NE (2008) Testicular dysgenesis syndrome and Leydig cell function. Basic Clin Pharmcol Toxicol 102:155-161.
24. Kalra N, Kumar V (2004) c-Fos is a mediator of the c-Myc-induced apoptotic signaling in serum-deprived hepatoma cells via the p38 mitogen-activated protein kinase pathway. J Biol Chem 279:25313-25319.
25. Karagiannis A, Harsoulis F (2005) Gonadal dysfunction in systemic diseases. Euro J Endocrinol 152:501-513.
26. Krishnaiah D, Sarbatly R, Bono A (2007) Phytochemical antioxidants for health and medicine - A move towards nature. Biotechnol Molbiol Rev 1:97-104.
27. Li YC, Hu XQ, Xiao LJ, Hu ZY, Guo J, Zhang KY, Song XX, Liu YX (2006) An oligonucleotide microarray study on gene expression profile in mouse testis of experimental cryptorchidism. Front Biosci 11:2465-2482.
28. Liu J, Lin A (2005) Role of JNK activation in apoptosis: a double-edged sword. Cell Res 15:36-42.
29. Lum HK, Butt YKC, Lo SCL (2002) Hydrogen peroxide induces a rapid production of nitric oxide in Mung Bean (Phaseolus aureus) . Nitric Oxide: Biol Chem 6:205-213.
30. Luo L, Chen H, Trush MA, Show MD, Anway MD, Zirkin BR (2006) Aging and the Brown Norway rat Leydig cell antioxidant defense system. J Androl 27:240-247.
31. Lyle N, Bhattacharyya D, Sur TK, Munshi S, Paul S, Chatterjee S, Gomes A (2009) Stress modulating antioxidant effect of Nardostachys jatamansi . Ind J Biochem Biophys 46:93-98.
32. Lysiak JJ, Turner SD, Nguyen QA, Singbartl K, Ley K, Turner TT (2001) Essential role of neutrophils in germ cell-specific apoptosis following ischemia/reperfusion injury of the mouse testis. Biol Reprod 65:718-725.
33. Lysiak JJ, Zheng S, Woodson R, Turner TT (2007) Caspase-9-dependent pathway to murine germ cell apoptosis: mediation by oxidative stress, BAX, and caspase-2. Cell Tissue Res 328:411-419.
34. 2 in vitro . FEBS J 276:870-881.
35. 2 induced germ cell apoptosis through down-regulation of caspase-9 and JNK/c-Jun. Mol Reprod Dev 78:69-79.
36. Mallick C, Mandal S, Barik B, Bhattacharya A, Ghosh D (2007) Protection of testicular dysfunctions by MTEC, a formulated herbal drug, in streptozotocin induced diabetic rat. Biol Pharm Bull 30:84-90.
37. Mallick C, Chatterjee K, Mandal U, Ghosh D (2008) Protective effects of MTEC, a formulated herbal drug on glycemic indices and testicular dysfunctions in streptozotocin induced diabetic rat. J Herbs Spices Med Plants 13:71-93.
38. Manda K, Ueno M, Moritake T, Anzai K (2007) Alpha-lipoic acid attenuates x-irradiation-induced oxidative stress in mice. Cell Biol Toxicol 23:129-137.
39. McClusky LM, de Jager C, Bornman MS (2007) Stage-related increase in the proportion of apoptotic germ cells and altered frequencies of stages in the spermatogenic cycle following gestational, lactational and direct exposure of male rats to p-nonylphenol. Toxicol Sci 95:249-256.
40. Mehta A, Sekhon CPS, Giri S, Orak JK, Singh AK (2002) Attenuation of ischemia/reperfusion induced MAP kinase by N-acetyl cysteine, sodium nitroprusside and phosphoramidon. Mol Cell Biochem 240:19-29.
41. Misro MM, Chaki SP, Gautam DK (2005) Germ cell death and their removal during initial stages of testicular ischemia and cryptorchidism: a comparative analysis. Ind J Exp Biol 43:1080-1087.
42. Morishima N, Nakanish K, Takenouchi H, Shibata T, Yasuhiko Y (2002) An endoplasmic reticulum stress-specific caspase cascade in apoptosis. J Biol Chem 277:34287-34294.
43. Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxidation in animal tissues by thiobarbituric acid reaction. Anal Biochem 9:351-358.
44. Petersen PM, Giwercman A, Hansen SW, Berthelsen JG, Daugaard G, Rorth M, Skakkebaek NE (1999) Impaired testicular function in patients with carcinoma in situ of the testis. J Clin Oncol 17:173-179.
45. Samanta L, Chainy GB (1997) Comparison of hexachlorocyclohexane-induced oxidative stress in the testis of immature and adult rats. Comp Biochem Physiol C Pharmacol Toxicol Endocrinol 118:319-327.
46. Santoro G, Romeo C (2001) Normal and varicocele testis in adolescents. Asian J Androl 3:259-262.
47. Sharma SB, Nasir A, Prabhu KM, Murthy PS (2006) Antihyperglycemic effect of the fruit-pulp of Eugenia jambolana in experimental diabetes mellitus. J Ethnopharmacol 104:367-373.
48. Sharma SB, Murthy PS, Prabhu KM, Nasir A (2009) Indian Product Patent 2, 30, 753 (to ICMR), February.
49. Sharma SB, Rajpoot R, Nasir A, Prabhu KM, Murthy PS (2010) Ameliorative effect of active principle isolated from seeds of Eugenia jambolana on carbohydrate metabolism in experimental diabetes. Evid Based Complement Alternat Med 2010:1-9.
50. Sohal RS, Agarwal S, Sohal BH (1995) Oxidative stress and aging in the Mongolian gerbil (Meriones unguiculatus). Mech Ageing Dev 81:15-25.
51. Son YO, Jang YS, Shi X, Lee JC (2009) Activation of JNK and c-Jun is involved in glucose oxidase-mediated cell death of human lymphoma cells. Mol Cells 28:545-551.
52. Stone JR, Yang S (2006) Hydrogen peroxide: a signaling messenger. Antioxid Redox Signal 8:243-270.
53. Syntin P, Chen H, Zirkin BR, Robaire B (2001) Gene expression in Brown Norway rat Leydig cells: effects of age and age-related germ cell loss. Endocrinology 142:5277-5285.
54. Tanwar RS, Sharma SB, Singh UR, Prabhu KM (2010) Attenuation of renal dysfunction by anti-hyperglycemic compound isolated from fruit pulp of Eugenia jambolana in streptozotocin-induced diabetic rats. Ind J Biochem Biophys 47:83-89.
55. Turner TT, Lysiak JJ (2008) Oxidative stress: a common factor in testicular dysfunction. J Androl 29:488-498.
56. Vasi S, Austin A (2009) Antioxidant potential of Eugenia jambolana Lam. Seeds. J Biol Sci 9:894-898.