Optimization of TNF-α overexpression in Escherichia coli using response surface methodology: Purification of the protein and oligomerization studies

Protein Expression and Purification

Volumn 86, Issue 1, 2012, Pages 35-44

  Papaneophytou, Christos P ^a   Kontopidis, George A ^a,b  

^a Centre for Research and Technology of Thessaly (CERETETH)   (Greece)

^b UNIVERSITY OF THESSALY   (Greece)

Author keywords

Induction conditions; Oligomerization; Response surface methodology; Tumor necrosis factor alpha

Indexed keywords

3C PROTEASES; CYSTEINE PROTEINASE; GLYCEROL; HYBRID PROTEIN; ISOPROPYL THIOGALACTOSIDE; POLYSORBATE; SODIUM CHLORIDE; TUMOR NECROSIS FACTOR ALPHA; VIRUS PROTEIN;

ARTICLE; BIOSYNTHESIS; CHEMISTRY; ESCHERICHIA COLI; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; PROTEIN DEGRADATION; PROTEIN MULTIMERIZATION; PROTEOMICS; SOLUBILITY; TEMPERATURE; TIME; TITRIMETRY;

CYSTEINE ENDOPEPTIDASES; ESCHERICHIA COLI; GLYCEROL; HUMANS; ISOPROPYL THIOGALACTOSIDE; POLYSORBATES; PROTEIN MULTIMERIZATION; PROTEOLYSIS; PROTEOMICS; RECOMBINANT FUSION PROTEINS; SODIUM CHLORIDE; SOLUBILITY; TEMPERATURE; TIME FACTORS; TITRIMETRY; TUMOR NECROSIS FACTOR-ALPHA; VIRAL PROTEINS;

EID: 84867840421     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2012.09.002     Document Type: Article

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