Enhanced expression of the recombinant lethal factor of Bacillus anthracis by fed-batch culture

Biochemical and Biophysical Research Communications

Volumn 285, Issue 4, 2001, Pages 1025-1033

  Gupta, Pankaj ^a   Sahai, Vikram ^b   Bhatnagar, Rakesh ^a  

^a JAWAHARLAL NEHRU UNIVERSITY   (India)

^b INDIAN INSTITUTE OF TECHNOLOGY DELHI   (India)

Author keywords

[No Author keywords available]

Indexed keywords

DISSOLVED OXYGEN; EXOTOXIN; RECOMBINANT PROTEIN;

ARTICLE; BACILLUS ANTHRACIS; BACTERIAL GROWTH; BACTERIUM CULTURE; FERMENTATION; LETHAL GENE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN ISOLATION; STRUCTURAL GENE;

EID: 0034816285     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2001.5282     Document Type: Article

References (33)

1. Activation of phospholipase C and protein kinase C is required for expression of anthrax toxin cytotoxicity in J774A.1 cells
2. Calcium is required for the expression of anthrax lethal toxin activity in the macrophage like cell line J774A.1
3. Protein synthesis is required for expression of anthrax lethal toxin cytotoxicity
4. Nucleotide sequence and analysis of the lethal factor gene (lef) from Bacillus anthracis
5. Proteolytic inactivation of MAP-kinase-kinase by anthrax lethal factor
6. Macrophages are sensitive to anthrax toxin through an acid dependent process
7. Characterization of macrophage sensitivity and resistance to anthrax lethal toxin
8. Growth of E. coli to high cell concentration by oxygen level linked control of carbon source concentration
9. Demonstration of a capsule plasmid in B. anthracis
10. Expression and purification of the recombinant lethal factor of Bacillus anthracis
11. Expression and purification of the recombinant protective antigen of B. anthracis
12. Involvement of residues 147VYYEIGK153 in binding of lethal factor to protective antigen of Bacillus anthracis
13. Biochemical and physiological changes induced by anthrax lethal toxin in J774A.1 macrophage like cells
14. Anthrax toxin lethal factor contains a zinc metalloprotease consensus sequence which is required for lethal toxin activity
15. Anthrax toxin protective antigen is activated by a cell surface protease with the sequence specificity and catalytic properties of furin
16. Simple fed batch technique for high cell density cultivation of E. coli
17. Anthrax toxin edema factor: A bacterial adenylate cyclase that increases cAMP conc. in eukaryotic cells
18. Production and purification of anthrax toxin
19. The anthrax toxin complex
20. Anthrax toxins
21. Protein measurement with the folin phenol reagent
22. Evidence for plasmid mediated toxin production in B. anthracis
23. Protective antigen binding domain of anthrax lethal factor mediates translocation of a heterologous protein fused to its amino or carboxy terminus
24. Purification of anthrax toxin components by high performance anion-exchange, gel filtration and hydrophobic interaction chromatography
25. Molecular cloning and expression in E. coli of the lethal factor gene of B. anthracis
26. Toxin structure and function
27. A deleted variant of B. anthracis protective antigen is non toxic and blocks anthrax toxin action in vivo
28. Observations on experimental anthrax: Demonstration of a specific lethal factor produced in vivo by Bacillus anthracis
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30. Purification of factor 1 and recognition of a third factor of anthrax toxin
31. Factors influencing productivity of fermentations employing recombinant microorganisms
32. Effect of fermentation feeding strategies prior to induction of expression of recombinant malaria antigen in E. coli