Recovery of soluble protein after expression in Escherichia coli depends on cellular disruption conditions

Microbios

Volumn 85, Issue 345, 1996, Pages 205-212

  González, Carlos ^a   Lagos, Rosalba ^b   Monasterio, Octavio ^b  

^a UNIVERSITY OF CONCEPCIÓN   (Chile)

^b UNIVERSITY OF CHILE   (Chile)

Author keywords

Cell disruption; Inclusion bodies; Nonionic detergents; Recombinant tubulin

Indexed keywords

OCTOXINOL; RECOMBINANT PROTEIN; SODIUM CHLORIDE;

ARTICLE; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; SOLUBILITY;

ESCHERICHIA COLI; OCTOXYNOL; RECOMBINANT PROTEINS; SODIUM CHLORIDE; SOLUBILITY;

EID: 0029693824     PISSN: 00262633     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. BABBITT P. C., West B. L., Buechter D. D., Kuntz I. D. and Kenyon G. L. 1990. Removal of a proteolytic activity associated with aggregates formed from expression of creatine kinase in Escherichia coli leads to improved recovery of active enzyme. Biotechnology 8 945-9.
2. ab-fragments produced in Escherichia coli. Biotechnology 9 157-62.
3. DUNDERDALE H. J., Sharples G. J., Lloyd R. G. and West S. C. 1994. Cloning, overexpression, purification, and characterization of the Escherichia coli RuvC Holliday junction resolvase. J. biol. Chem. 269 5187-94.
4. FRANKEL S., Sohn R. and Leinwand L. 1991. The use of sarkosyl in generating soluble protein after bacterial expression. Proc. natn. Acad. Sci. U.S.A. 88 1192-6.
5. GAREL M-C., Joulin V., Le Boulch P., Calvin M-C., Préhu M-O., Arous N., Longin R., Rosa R., Rosa J. and Cohen-Solal M. 1989. Human bisphosphoglycerate mutase. J. biol. Chem. 264 18966-72.
6. GENTRY D. R. and Burgess R. R. 1990. Overproduction and purification of the ω subunit of Escherichia coli RNA polymerase. Protein Expression Purification 1 81-6.
7. HOESS A., Arthur A. K., Wanner G. and Fanning E. 1988. Recovery of soluble, biologically active recombinant proteins from total bacterial lysates using ion exchange resin. Biotechnology 6 1214-7.
8. MARSTON F. A. O. 1986. The purification of eukaryotic polypeptides synthesized in Escherichia coli. Biochem. J. 240 1-12.
9. MILLER J. H. 1972. Experiments in Molecular Genetics. Cold Spring Harbor Press.
10. SCHAGGER H. and von Jagow G. 1987. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Analyt. Biochem. 166 368-79.
11. SCHEIN C. H. 1989. Production of soluble recombinant proteins in bacteria. Biotechnology 7 1141-7.
12. STUDIER F. W. and Moffatt B. A. 1986. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. molec. Biol. 189 113-30.
13. TABOR S. and Richardson C. C. 1985. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proc. natn. Acad. Sci. U.S.A. 82 1074-8.
14. VALENZUELA P., Quiroga M., Zaldivar J., Rutter W. J., Kirschner M. W. and Cleveland D. W. 1981. Nucleotide and corresponding amino acid sequences encoded by α and β tubulin mRNAs. Nature 289 650-5.
15. WILKINSON D. L. and Harrison R. G. 1991. Predicting the solubility of recombinant proteins in Escherichia coli. Biotechnology 9 443-8.
16. WU J. and Yarbrough L. R. 1987. Expression of the alpha and beta tubulin genes of the African trypanosome in Escherichia coli. Gene 61 51-62.