A new crystal form of MshB from Mycobacterium tuberculosis with glycerol and acetate in the active site suggests the catalytic mechanism

Acta Crystallographica Section D: Biological Crystallography

Volumn 68, Issue 11, 2012, Pages 1450-1459

  Broadley, Simon Gareth ^a   Gumbart, James Conrad ^b   Weber, Brandon William ^a   Marakalala, Mohlopheni Jackson ^a   Steenkamp, Daniel Jacobus ^a   Sewell, Bryan Trevor ^a  

^a UNIVERSITY OF CAPE TOWN   (South Africa)

^b ARGONNE NATIONAL LABORATORY   (United States)

Author keywords

catalytic mechanism; conformational change; hydrolases; MshB; Mycobacterium tuberculosis; oxyanion hole

Indexed keywords

ACETIC ACID DERIVATIVE; AMIDASE; AMMONIUM SULFATE; BACTERIAL PROTEIN; GLYCEROL; MACROGOL DERIVATIVE; N ACETYL 1 D INOSITYL 2 AMINO 2 DEOXY ALPHA D GLUCOPYRANOSIDE DEACETYLASE; N-ACETYL-1-D-INOSITYL-2-AMINO-2-DEOXY-ALPHA-D-GLUCOPYRANOSIDE DEACETYLASE;

ARTICLE; CHEMISTRY; ENZYME ACTIVE SITE; METABOLISM; MICROBIOLOGY; MOLECULAR DYNAMICS; MYCOBACTERIUM TUBERCULOSIS; PROTEIN CONFORMATION; TUBERCULOSIS; X RAY CRYSTALLOGRAPHY;

ACETATES; AMIDOHYDROLASES; AMMONIUM SULFATE; BACTERIAL PROTEINS; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; GLYCEROL; MOLECULAR DYNAMICS SIMULATION; MYCOBACTERIUM TUBERCULOSIS; POLYETHYLENE GLYCOLS; PROTEIN CONFORMATION; TUBERCULOSIS;

EID: 84867816333     PISSN: 09074449     EISSN: 13990047     Source Type: Journal    
DOI: 10.1107/S090744491203449X     Document Type: Article

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