Short-chain peptides identification of scorpion Buthus martensi Karsch venom by employing high orthogonal 2D-HPLC system and tandem mass spectrometry

Proteomics

Volumn 12, Issue 19-20, 2012, Pages 3076-3084

  Xu, Junyan ^a,b   Zhang, Xiuli ^a   Guo, Zhimou ^a   Yan, Jingyu ^a   Yu, Long ^a   Li, Xiuling ^a   Xue, Xingya ^a   Liang, Xinmiao ^a  

^a DALIAN INSTITUTE OF CHEMICAL PHYSICS   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

Hydrophilic interaction chromatography; Peptide separation; Scorpion venom; Tandem mass spectrometry; Two dimensional liquid chromatography

Indexed keywords

BUTHUS MARTENSI KARSCH VENOM; MARTENTOXIN; MARTENTOXIN B; PEPTIDE; PEPTIDE BMKK12; PEPTIDE BMKK16; PEPTIDE BMKK2; PEPTIDE BMKK2 B; POTASSIUM CHANNEL; POTASSIUM CHANNEL TOXIN ALPHA KTX17; SCORPION VENOM; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; COLLISIONALLY ACTIVATED DISSOCIATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROPHILIC INTERACTION CHROMATOGRAPHY; PRIORITY JOURNAL; PROTEIN ANALYSIS; TANDEM MASS SPECTROMETRY;

AMINO ACID SEQUENCE; ANIMALS; CHLORIDE CHANNELS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PEPTIDES; POTASSIUM CHANNEL BLOCKERS; PROTEOMICS; SCORPION VENOMS; SCORPIONS; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, PROTEIN; TANDEM MASS SPECTROMETRY;

EID: 84867814811     PISSN: 16159853     EISSN: 16159861     Source Type: Journal    
DOI: 10.1002/pmic.201200224     Document Type: Article

References (41)

1. Possani, L. D., Becerril, B., Delepierre, M., Tytgat, J., Scorpion toxins specific for Na+-channels. Eur. J. Biochem. 1999, 264, 287-300.
2. Lewis, R. J., Garcia, M. L., Therapeutic potential of venom peptides. Nat. Rev. Drug Discov. 2003, 2, 790-802.
3. Han, S., Yi, H., Yin, S. J., Chen, Z. Y. et al., Structural basis of a potent peptide inhibitor designed for Kv1.3 channel, a therapeutic target of autoimmune disease. J. Biol. Chem. 2008, 283, 19058-19065.
4. Wang, C. S., St Leger, R. J., A scorpion neurotoxin increases the potency of a fungal insecticide. Nat. Biotechnol. 2007, 25, 1455-1456.
5. Goudet, C., Chi, C. W., Tytgat, J., An overview of toxins and genes from the venom of the Asian scorpion Buthus martensi Karsch. Toxicon 2002, 40, 1239-1258.
6. Batista, C. V. F., D'Suze, G., Gomez-Lagunas, F., Zamudio, F. Z. et al., Proteomic analysis of Tityus discrepans scorpion venom and amino acid sequence of novel toxins. Proteomics 2006, 6, 3718-3727.
7. Bringans, S., Eriksen, S., Kendrick, T., Gopalakrishnakone, P. et al., Proteomic analysis of the venom of Heterometrus longimanus (Asian black scorpion). Proteomics 2008, 8, 1081-1096.
8. Tang, X., Zhang, Y. Q., Hu, W. J., Xu, D. H. et al., Molecular diversification of peptide toxins from the Tarantula Haplopelma hainanum (Ornithoctonus hainana) venom based on transcriptomic, peptidomic, and genomic analyses. J. Proteome Res. 2010, 9, 2550-2564.
9. Li, M. H., Zhang, N. X., Chen, X. Q., Wu, G. et al., Purification and pharmacological characterization of BmKK2 (alpha-KTx 14.2), a novel potassium channel-blocking peptide, from the venom of Asian scorpion Buthus martensi Karsch. Toxicon 2004, 43, 895-900.
10. Gao, R., Zhang, Y., Gopalakrishnakone, P., Purification and N-terminal sequence of a serine proteinase-like protein (BMK-CBP) from the venom of the Chinese scorpion (Buthus martensii Karsch). Toxicon 2008, 52, 348-353.
11. 2+-activated K+ channels. Eur. J. Biochem. 1997, 245, 457-464.
12. RomiLebrun, R., Lebrun, B., MartinEauclaire, M. F., Ishiguro, M. et al., Purification, characterization, and synthesis of three novel toxins from the Chinese scorpion Buthus martensi, which act on K+ channels. Biochemistry 1997, 36, 13473-13482.
13. Chen, Z. Y., Reddy, G., Hahin, R., The isolation and purification of two peptides from the venom of Buthus martensii Karsch. Toxicon 2000, 38, 1817-1832.
14. Opiteck, G. J., Jorgenson, J. W., Anderegg, R. J., Two-dimensional SEC/RPLC coupled to mass spectrometry for the analysis of peptides. Anal. Chem. 1997, 69, 2283-2291.
15. Shen, Y. F., Jacobs, J. M., Camp, D. G., Fang, R. H. et al., Ultra-high-efficiency strong cation exchange LC/RPLC/MS/MS for high dynamic range characterization of the human plasma proteome. Anal. Chem. 2004, 76, 1134-1144.
16. Stoll, D. R., Carr, P. W., Fast, comprehensive two-dimensional HPLC separation of tryptic peptides based on high-temperature HPLC. J. Am. Chem. Soc. 2005, 127, 5034-5035.
17. Nascimento, D. G., Rates, B., Santos, D. M., Verano-Braga, T. et al., Moving pieces in a taxonomic puzzle: venom 2D-LC/MS and data clustering analyses to infer phylogenetic relationships in some scorpions from the Buthidae family (Scorpiones). Toxicon 2006, 47, 628-639.
18. Boersema, P. J., Divecha, N., Heck, A. J. R., Mohammed, S., Evaluation and optimization of ZIC-HILIC-RP as an alternative MudPIT strategy. J. Proteome Res. 2007, 6, 937-946.
19. Gilar, M., Olivova, P., Daly, A. E., Gebler, J. C., Orthogonality of separation in two-dimensional liquid chromatography. Anal. Chem. 2005, 77, 6426-6434.
20. Arnold, K., Bordoli, L., Kopp, J., Schwede, T., The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling. Bioinformatics 2006, 22, 195-201.
21. Koradi, R., Billeter, M., Wuthrich, K., MOLMOL: a program for display and analysis of macromolecular structures. J. Mol. Graph. 1996, 14, 51-55.
22. Fu, Q., Guo, Z. M., Liang, T., Zhang, X. L. et al., Chemically bonded maltose via click chemistry as stationary phase for HILIC. Anal. Methods 2010, 2, 217-224.
23. Guo, Z. M., Lei, A. W., Zhang, Y. P., Xu, Q. et al., "Click saccharides'': novel separation materials for hydrophilic interaction liquid chromatography. Chem. Commun. 2007, 2491-2493.
24. Alpert, A. J., Electrostatic repulsion hydrophilic interaction chromatography for isocratic separation of charged solutes and selective isolation of phosphopeptides. Anal. Chem. 2008, 80, 62-76.
25. Mihailova, A., Malerod, H., Wilson, S. R., Karaszewski, B. et al., Improving the resolution of neuropeptides in rat brain with on-line HILIC-RP compared to on-line SCX-RP. J. Sep. Sci. 2008, 31, 459-467.
26. 2+-activated potassium channels from the scorpion Buthus martensi Karsch. FEBS Lett. 1999, 452, 360-364.
27. 2+-activated K+ channel. Toxicon 2004, 43, 961-971.
28. Li, M. H., Zhang, N. X., Chen, X. Q., Wu, G. et al., BmKK4, a novel toxin from the venom of Asian scorpion Buthus martensi Karsch, inhibits potassium currents in rat hippocampal, neurons in vitro. Toxicon 2003, 42, 199-205.
29. Cao, Z. Y., Shen, W. Q., Pan, Y. P., Xiao, X. et al., Purification, characterization of two peptides from Buthus martensi Karch. J. Pept. Res. 2003, 62, 252-259.
30. + channel: pharmacological characterization and localization in rat brain. FEBS Lett. 2001, 501, 31-36.
31. Zhang, N. X., Chen, X., Li, M. H., Cao, C. Y. et al., Solution structure of BmKK4, the first member of subfamily alpha-KTx 17 of scorpion toxins. Biochemistry 2004, 43, 12469-12476.
32. Aiyar, J., Rizzi, J. P., Gutman, G. A., Chandy, K. G., The signature sequence of voltage-gated potassium channels projects into the external vestibule. J. Biol. Chem. 1996, 271, 31013-31016.
33. Zhang, N. X., Li, M. H., Chen, X., Wang, Y. et al., Solution structure of BmKK2, a new potassium channel blocker from the venom of Chinese scorpion Buthus martensi Karsch. Proteins 2004, 55, 835-845.
34. Zeng, X. C., Luo, F., Li, W. X., Molecular dissection of venom from Chinese scorpion Mesobuthus martensii: identification and characterization of four novel disulfide-bridged venom peptides. Peptides 2006, 27, 1745-1754.
35. Korolkova, Y. V., Kozlov, S. A., Lipkin, A. V., Pluzhnikov, K. A. et al., An ERG channel inhibitor from the scorpion Buthus eupeus. J. Biol. Chem. 2001, 276, 9868-9876.
36. + channel with its specific BeKm-1 peptide: insights into the selectivity of molecular recognition. J. Proteome Res. 2007, 6, 611-620.
37. Devuyst, O., Guggino, W. B., Chloride channels in the kidney: lessons learned from knockout animals. Am. J. Physiol. Renal Physiol. 2002, 283, F1176-F1191.
38. Debin, J. A., Maggio, J. E., Strichartz, G. R., Purification and characterization of chlorotoxin, a chloride channel ligand from the venom of the scorpion. Am. J. Physiol. 1993, 264, C361-C369.
39. Soroceanu, L., Gillespie, Y., Khazaeli, M. B., Sontheimer, H., Use of chlorotoxin for targeting of primary brain tumors. Cancer Res. 1998, 58, 4871-4879.
40. Fan, S. Z., Sun, Z. B., Jiang, D. H., Dai, C. et al., BmKCT toxin inhibits glioma proliferation and tumor metastasis. Cancer Lett. 2010, 291, 158-166.
41. Veiseh, M., Gabikian, P., Bahrami, S. B., Veiseh, O. et al., Tumor paint: a chlorotoxin : Cy5.5 bioconjugate for intraoperative visualization of cancer foci. Cancer Res. 2007, 67, 6882-6888.