Molecular subdivision of the marine diatom Thalassiosira rotula in relation to geographic distribution, genome size, and physiology

BMC Evolutionary Biology

Volumn 12, Issue 1, 2012, Pages

  Whittaker, Kerry A ^a   Rignanese, Dayna R ^a   Olson, Robert J ^b   Rynearson, Tatiana A ^a  

^a UNIVERSITY OF RHODE ISLAND   (United States)

^b WOODS HOLE OCEANOGRAPHIC INSTITUTION   (United States)

Author keywords

Dispersal; Intraspecific diversity; Phylogeography; Physiology; Phytoplankton

Indexed keywords

CRYPSIS; DIATOM; GENOME; GENOTYPE; GEOGRAPHICAL DISTRIBUTION; GROWTH RATE; MOLECULAR ANALYSIS; MORPHOLOGY; PHYLOGENETICS; PHYLOGEOGRAPHY; PHYSIOLOGY; PHYTOPLANKTON;

ATLANTIC OCEAN; PACIFIC OCEAN;

BACILLARIOPHYTA; MYA; NITZSCHIA ALBA; THALASSIOSIRA; THALASSIOSIRA GRAVIDA; THALASSIOSIRA ROTULA;

RIBOSOMAL SPACER DNA; RIBOSOME DNA; RNA 28S;

ARTICLE; ATLANTIC OCEAN; CHEMISTRY; CLASSIFICATION; DIATOM; DNA SEQUENCE; GENETIC VARIABILITY; GENETICS; GENOME SIZE; GEOGRAPHY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PACIFIC OCEAN; PHYLOGENY; PHYSIOLOGY; SPECIES DIFFERENCE;

ATLANTIC OCEAN; BASE SEQUENCE; DIATOMS; DNA, RIBOSOMAL; DNA, RIBOSOMAL SPACER; GENETIC VARIATION; GENOME SIZE; GEOGRAPHY; MOLECULAR SEQUENCE DATA; PACIFIC OCEAN; PHYLOGENY; RNA, RIBOSOMAL, 28S; SEQUENCE ANALYSIS, DNA; SPECIES SPECIFICITY;

MLCS; MLOWN;

EID: 84867812456     PISSN: None     EISSN: 14712148     Source Type: Journal    
DOI: 10.1186/1471-2148-12-209     Document Type: Article

References (99)

1. Photosynthesis in Global-Scale Models. Laisk A, Nedbal L, Govindjee, Friend AD, Geider RJ, Behrenfeld MJ, Still CJ, Photosynthesis in silico Netherlands: Springer Netherlands, Govindjee, 29 2009 465 497
2. Primary Production of the Biosphere: Integrating Terrestrial and Oceanic Components. Field CB, Sci 1998 281 5374 237 240 (Pubitemid 28334501)
3. Glacial migrations of plants: island biogeographical evidence. Simpson BB, Sci 1974 185 4152 698 700 10.1126/science.185.4152.698
4. Spatial Moment Equations for Plant Competition: Understanding Spatial Strategies and the Advantages of Short Dispersal. Bolker BM, Pacala SW, Am Nat 1999 153 6 575 602 10.1086/303199 (Pubitemid 29347838)
5. Southern Hemisphere Biogeography Inferred by Event-Based Models: Plant versus Animal Patterns. Sanmartin I, Ronquist F, Syst Biol 2004 53 2 216 243 10.1080/10635150490423430 15205050 (Pubitemid 38611181)
6. Why are there so many kinds of flowering plants? Burger WC, Bioscience 1981 31 8 572 10.2307/1308218
7. Diversity and evolution of marine phytoplankton. Simon N, Cras A-L, Foulon E, Lemee R, C R Biol 2009 332 2-3 159 19281949
8. Propagule dispersal in marine and terrestrial environments: a community perspective. Kinlan BP, Gains SD, Ecol 2003 84 8 2007 2020 10.1890/01-0622 (Pubitemid 37435675)
9. Comparing Marine and Terrestrial Ecosystems: Implications for the Design of Coastal Marine Reserves. Carr MH, Neigel JE, Estes JA, Andelman S, Warner RR, Largier JL, Ecol Appl 2003 13 1 90 S107 10.1890/1051-0761(2003)013[0090:CMATEI] 2.0.CO;2
10. Production and dissolution of biogenic silica in the ocean: Revised global estimates, comparison with regional data and relationship to biogenic sedimentation. Nelson DM, Treguer P, Brzezinski MA, Leynaert A, Queguiner B, Glob Biogeochem Cycle 1995 9 3 359 372 10.1029/95GB01070 (Pubitemid 26439874)
11. Biodiversity, biogeography and conservation of diatoms. Mann DG, Droop SJM, Hydrobiologia 1996 336 1 19 10.1007/BF00010816
12. A nuclear-encoded small-subunit ribosomal RNA timescale for diatom evolution. Sorhannus U, Mar Micropaleontol 2007 65 1-2 1 12 (Pubitemid 47451667)
13. Round F, Crawford R, Mann D, The Diatoms: biology and morphology of the genera Cambridge, UK: Cambridge University Press 1990
14. Pseudocryptic diversity versus cosmopolitanism in diatoms: a case study on Navicula cryptocephala Kutz. (Bacillariophyceae) and morphologically similar taxa. Poulickova A, Vesela J, Neustupa J, Skaloud P, Protist 2010 161 353 369 10.1016/j.protis.2009.12.003 20097131
15. Reproductive Isolation among Sympatric Cryptic Species in Marine Diatoms. Amato A, Kooistra WHCF, Levialdi Ghiron JH, Mann DG, Proschold T, Montresor M, Protist 2007 158 2 193 207 10.1016/j.protis.2006.10.001 17145201 (Pubitemid 46467505)
16. The species concept in a marine diatom: LSU rDNA- based phylogenetic differentiation in Skeletonema marinoi/dohrnii (Bacillariophyceae) is not reflected in morphology. Ellegaard M, Godhe A, Härnström K, McQuoid M, Phycologia (Oxford) 2008 47 2 156 167 (Pubitemid 351466478)
17. Molecular systematics and the diatom species. Alverson AJ, Protist 2008 159 3 339 353 10.1016/j.protis.2008.04.001 18539524
18. Diversity in the genus Skeletonema (Bacillariophyceae). An assessment of the taxonomy of S. costatum-like species with the description of four new species. Sarno D, Kooistra W, Medlin L, Percopo I, Zingone A, J Phycol 2005 41 1 151 176 10.1111/j.1529-8817.2005.04067.x (Pubitemid 40263635)
19. Diversity in the genus Skeletonema (Bacillariophyceae). I. A reexamination of the type material of S. costatum with the description of S. grevillei sp. Zingone A, Percopo I, Sims PA, Sarno D, J Phycol 2005 41 1 140 150 10.1111/j.1529-8817.2005.04066.x (Pubitemid 40263634)
20. Diversity in the genus Skeletonema (Bacillariophyceae) III. Phylogenetic position and mophological variability of Skeletonema grevillei, with the description of Skeletonema ardens sp. Sarno D, Kooistra WHCF, Balzano S, Hargraves PE, Zingone A, J Phycol 2007 43 1 156 170 10.1111/j.1529-8817.2006. 00305.x (Pubitemid 46232949)
21. An sssessment of potential diatom barcode genes (cox1, rbcL, 18S and ITS rDNA) and their effectiveness in determining relationships in Sellaphora (Bacillariophyta). Evans KM, Wortley AH, Mann DG, Protist 2007 158 3 349 364 10.1016/j.protis.2007.04.001 17581782 (Pubitemid 47001774)
22. Barcoding micro- and meso-fauna. Barcoding diatoms: Is there a good marker? Moniz MB, Kaczmarska I, Mol Ecol Resour 2009 9 1 65 74 21564966
23. ITS2 is a double-edged tool for eukaryote evolutionary comparisons. Coleman AW, Trends Genet 2003 19 7 370 10.1016/S0168-9525(03)00118-5 12850441 (Pubitemid 36794538)
24. The significance of a coincidence between evolutionary landmarks found in mating affinity and a DNA sequence. Coleman AW, Protist 2000 151 1 1 9 10.1078/1434-4610-00002 10896128 (Pubitemid 30354786)
25. Reproductive compatibility and rDNA sequence analysis in the Sellaphora pupula species complex (Bacillariophyta). Behnke A, Friedl T, Chepurnov VA, Mann DG, J Phycol 2004 40 1 193 208 10.1046/j.1529-8817.2004.03037.x (Pubitemid 38241650)
26. Comparative evolutionary analysis of rDNA ITS regions in drosophila. Schlötterer C, Hauser M-T, von Haesler A, Tautz D, Mol Biol Evol 1994 11 513 533 8015444
27. The species concept in a marine diatom: LSU rDNA based phylogenetic differentiation in Skeletonema marinoi/dohrnii (Bacillariophyceae) is not reflected in morphology. Ellegaard M, Godhe A, Härnstrom K, McQuoid M, Phycologia (Oxford) 2008 47 2 156 167 (Pubitemid 351466478)
28. Genome size differentiates co-occurring populations of the planktonic diatom Ditylum brightwellii (Bacillariophyta). Koester JA, Swalwell JE, von Dassow P, Armbrust EV, BMC Evol Biol 2010 10 1 10.1186/1471-2148-10-1 20044934
29. Inter-and intraspecific relationships between nuclear DNA content and cell size in selected members of the centric diatom genus Thalassiosira (Bacillariophyceae). Von Dassow P, Petersen TW, Chepernov VA, Armbrust EV, J Phycol 2008 44 2 335 349 10.1111/j.1529-8817.2008.00476.x (Pubitemid 351548575)
30. The frequency of polyploid speciation in vascular plants. Wood TE, Takebayashi N, Barker MS, Mayrose I, Greenspoon PB, Rieseberg LH, PNAS 2009 106 33 13875 13879 10.1073/pnas.0811575106 19667210
31. Biogeographical studies of marine phytoplankton. Smayda TJ, Oikos 1958 9 2 158 10.2307/3564763
32. Global diversity and biogeography of Skeletonema species (Bacillariophyta). Kooistra WHCF, Sarno D, Balzano S, Gu H, Andersen RA, Zingone A, Protist 2008 159 2 177 10.1016/j.protis.2007.09.004 18042429 (Pubitemid 351406512)
33. Inter and intraspecific community structure within the diatom genus Pseudo-nitzschia (Bacillariophyceae). Hubbard KA, Rocap GE, Armbrust V, J Phycol 2008 44 3 637 649 10.1111/j.1529-8817.2008.00518.x (Pubitemid 351743558)
34. Pseudo-nitzschia pungens (Bacillariophyceae): a cosmopolitan diatom species? Casteleyn G, Chepurnov VA, Leliaert F, Mann DG, Stephen S, Bates SS, Lundholm N, Rhodes L, Sabbe K, Vyverman W, Harmful Algae 2008 7 2 241 10.1016/j.hal.2007.08.004 (Pubitemid 350235339)
35. Controls on tropical Pacific Ocean productivity revealed through nutrient stress diagnostics. Behrenfeld MJ, Worthington K, Sherrell RM, Chavez FP, Strutton P, McPhaden M, Shea DM, Nature 2006 442 7106 1025 1028 10.1038/nature05083 16943835 (Pubitemid 44330257)
36. Phytoplankton biogeography and community stability in the ocean. Cermeno P, de Vargas C, Abrantes F, Falkowski PG, PLoS One 2010 5 4 10037 10.1371/journal.pone.0010037
37. Ocean climate change, phytoplankton community responses, and harmful algal blooms: a formidable predictive challenge. Hallegraeff GM, J Phycol 2010 46 2 220 235 10.1111/j.1529-8817.2010.00815.x
38. Parallel plastic tank experiments with cultures of marine diatoms. Brockmann U, Eberlein K, Hentzschel G, Schöne H, Siebers D, Wandschneider K, Weber A, Helgol Mar Res 1977 30 1 201 216
39. The biogeography of some marine planktonic diatoms. Hasle GR, Deep Sea Res Oceanograph Abstr 1976 23 4 319 338 IN311-IN316 10.1016/0011-7471(76)90873-1
40. Phytoplankton of the Calanus expedition in Hudson Bay, 1953 and 1954. Bursa A, J Fish Res Bd Can 1961 18 51 83 10.1139/f61-004
41. Seasonal changes in diatoms and dinoflagellates of the east coast of New Zealand during 1957 and 1958. Cassie V, NZJSci 1960 3 137 172
42. Cooperative studies on primary production in coastal waters of Japan 1962-1963. Matsudaira Y, Inform Bull Planktol Japan 1964 11 24 73
43. The phytoplankton of Narragansett Bay. Pratt DM, Limnol Oceanogr 1959 4 4 425 10.4319/lo.1959.4.4.0425
44. Autecology and clonal variability of the marine centric diatom Thalassiosira rotula; (Bacillariophyceae) in response to light, temperature and salinity. Krawiec RW, Mar Biol 1982 69 1 79 89 10.1007/BF00396964
45. Phytoplankton studies in lower Narragansett Bay. Smayda TJ, Limnol Oceanogr 1957 2 4 342
46. The diatoms and the physical and chemial conditions of the seawater of the San Juan Archipelago. Gran H, Thompson T, Puget Sound Biol 1930 7 169 204
47. Microplancton des Mers de Berents et de Kara. Meunier A, Duc d'Orleans Champagne Artique de 1907 1910 Atlas of pls I-XXXVII 1 355 21951770
48. Redogörelse för de svenska hydrografiska undersökningarne februari 1896 V. Planktonundersökningar: Vegetabilskt plankton. Cleve PT, Bihang till Kungliga Svenska Vetenskapsakademiens Handlingar 1896 22 5 1 33
49. Thalassiosira rotula and T. gravida: ecology and morphology. Syvertsen EE, Nova Hedwigia Beihefte 1977 57 99 112
50. Thalassiosira rotula, a heterotypic synonym of Thalassiosira gravida: morphological evidence. Sar EA, Sunesen I, Lavigne AS, Lofeudo S, Diatom Res 2011 26 1 109 119 10.1080/0269249X.2011.573691
51. Guillard RR, Culture of phytoplankton for feeding marine invertebrates Plenum Book Publ. Corp., New York 1975 In: W. L. Smith and M. H. Chanley, eds., Culture of marine invertebrate animals. P. 29-60
52. Spring bloom development, genetic variation, and population succession in the planktonic diatom Ditylum brightwellii. Rynearson TA, Newton JA, Armbrust EV, Limnol Oceanogr 2006 51 3 1249 10.4319/lo.2006.51.3.1249
53. The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. Medlin L, Elwood HJ, Stickel S, Sogin ML, Gene 1988 71 2 491 499 10.1016/0378-1119(88)90066-2 3224833 (Pubitemid 19010867)
54. Structure of the large subunit rDNA from a diatom, and comparison between small and large subunit ribosomal RNA for studying stramenopile evolution. Auwera GVD, Wachter RD, J Eukaryot Microbiol 1998 45 5 521 527 10.1111/j.1550-7408.1998.tb05111.x 9783453
55. Genetic differentiation among populations of the planktonic marine diatom Ditylum brightwellii (Bacillariophyceae). Rynearson TA, Armbrust EV, J Phycol 2004 40 1 34 43 10.1046/j.1529-8817.2004.03089.x (Pubitemid 38241634)
56. Rapid evolution of a sexual reproduction gene in centric diatoms of the genus Thalassiosira. Armbrust EV, Galindo HM, Appl Environ Microbiol 2001 67 8 3501 3513 10.1128/AEM.67.8.3501-3513.2001 11472926 (Pubitemid 33641221)
57. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Thompson JD, Higgins DG, Gibson TJ, Nucleic Acids Res 1994 22 22 4673 4680 10.1093/nar/22.22.4673 7984417 (Pubitemid 24354800)
58. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0. Tamura K, Dudley J, Nei M, Kumar S, Mol Biol Evol 2007 24 8 1596 1599 10.1093/molbev/msm092 17488738 (Pubitemid 47236692)
59. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Kimura M, J Mol Evol 1980 16 2 111 120 10.1007/BF01731581 7463489 (Pubitemid 11166431)
60. An exact test for population differentiation. Raymond M, Rousset F, Evolution 1995 49 6 1280 10.2307/2410454
61. Arlequin (version 3.0): an integrated software package for population genetics data analysis. Excoffier L, Laval LG, Schneider S, Evol Bioinform Online 2005 1 47
62. Expanded sequence dependence of thermodynamic parameters improves prediction of RNA secondary structure1. Mathews DH, Sabina J, Zuker M, Turner DH, J Mol Biol 1999 288 5 911 10.1006/jmbi.1999.2700 10329189 (Pubitemid 29248642)
63. RDP3: a flexible and fast computer program for analyzing recombination. Martin DP, Lemey P, Lott M, Moulton V, Posada D, Lefeuvre P, Bioinformatics 2010 26 19 2462 2463 10.1093/bioinformatics/btq467 20798170
64. DNA fingerprinting reveals extensive genetic diversity in a field population of the centric diatom Ditylum brightwellii. Rynearson TA, Armbrust EV, Limnol Oceanogr 2000 45 6 1329 1340 10.4319/lo.2000.45.6.1329
65. A method for the rapid and precise determination of acclimated phytoplankton reproduction rates. Brand LE, Guillard RRL, Murphy LS, J Plankton Res 1981 3 2 193 201 10.1093/plankt/3.2.193
66. The Analysis of Variance. Wiley, New York 1959, 477 Seiten. Enderlein G, Scheffé H, Biom Z 1961 3 2 143 144 10.1002/bimj.19610030206
67. One degree of freedom for non-additivity. Tukey JW, Biometrics 1949 5 3 232 242 10.2307/3001938
68. A submersible imaging-in-flow instrument to analyze nano and mciroplankton: Imaging FlowCytobot. Olson RJ, Sosik HM, Limnol Oceanogr Meth 2007 5 195 203 (Pubitemid 351773987)
69. Pan-eukaryote ITS2 homologies revealed by RNA secondary structure. Coleman AW, Nucleic Acids Res 2007 35 10 3322 3329 10.1093/nar/gkm233 17459886 (Pubitemid 47073596)
70. Secondary structure models for the internal transcribed spacer (ITS) region 1 from symbiotic dinoflagellates. Thornhill DJ, Lord JB, Protist 2010 161 3 434 451 10.1016/j.protis.2009.11.004 20106718
71. Pseudo-nitzschia pungens (Bacillariophyceae): A cosmopolitan diatom species? Casteleyn G, Chepurnov VA, Leliaert F, Mann DG, Bates SS, Lundholm N, Rhodes L, Sabbe K, Vyverman W, Harmful Algae 2008 7 2 241 257 10.1016/j.hal.2007.08.004 (Pubitemid 350235339)
72. Polyploidy: Doubling up for evolutionary success. Hegarty M, Hiscock S, Curr Biol 2007 17 21 927 R929 10.1016/j.cub.2007.08.060 17983572 (Pubitemid 350016911)
73. Genomic clues to the evolutionary success of polyploid plants. Hegarty MJ, Hiscock SJ, Curr Biol 2008 18 10 435 R444 10.1016/j.cub.2008.03.043 18492478 (Pubitemid 351672036)
74. The evolutionary consequences of polyploidy. Otto SP, Cell 2007 131 3 452 10.1016/j.cell.2007.10.022 17981114 (Pubitemid 350007701)
75. In polyploids, one plus one does not equal two. Otto SP, Trends Ecol Evol 2003 18 9 431 10.1016/S0169-5347(03)00213-1 (Pubitemid 37007499)
76. Hartl D, Clark A, Principles of Population Genetics Sunderland, MA: Sinauer Associates 1998
77. The time for sex: A biennial life cycle in a marine planktonic diatom. D'Alelio D, D'Alcala MR, Dubroca L, Sarno D, Zingone A, Montresor M, Limnol Oceanogr 2010 55 1 106 114 10.4319/lo.2010.55.1.0106
78. Sexual reproduction in the pennate diatoms Pseudo-nitzschia multiseries and P. pseudodelicatissima (Ballicariophyceae). Davidovich NA, Bates SS, J Phycol 1998 34 1 126 137 10.1046/j.1529-8817.1998.340126.x (Pubitemid 28176583)
79. The role of sex in the sedimentation of a marine diatom bloom. Crawford RM, Limnol Oceanogr 1995 40 200 204 10.4319/lo.1995.40.1.0200
80. The role of sinking and ascent during sexual reproduction in the marine diatom Ditylum brightwellii. Waite A, Harrison PJ, Mar Ecol Prog Ser 1992 87 113 122
81. The diatom sex clock and its evolutionary significance. Lewis WM Jr, Am Nat 1984 123 1 73 80 10.1086/284187 (Pubitemid 15272654)
82. Limits to gene flow in a cosmopolitan marine planktonic diatom. Casteleyn G, Leliaert F, Backeljau T, Debeer A-E, Kotaki Y, Rhodes L, Lundholm N, Sabbe K, Vyverman W, Proc Natl Acad Sci 2010 107 29 12952 12957 10.1073/pnas. 1001380107 20615950
83. Genetic divergence, reproductive Isolation, and marine Speciation. Palumbi SR, Annu Rev Ecol Syst 1994 25 1 547 572 10.1146/annurev.es.25.110194. 002555 (Pubitemid 24986131)
84. Phylogeography of North American populations of the moss species Hylocomium splendens based on the nucleotide sequence of internal transcribed spacer 2 of nuclear ribosomal DNA. Chiang TY, Schaal BA, Mol Ecol 1999 8 6 1037 1042 10.1046/j.1365-294x.1999.00668.x (Pubitemid 29337758)
85. Landscape approaches to historical and contemporary gene flow in plants. Sork VL, Nason J, Campbell DR, Fernandez JF, Trends Ecol Evol 1999 14 6 219 224 10.1016/S0169-5347(98)01585-7 10354623 (Pubitemid 29235003)
86. Metapopulation Structure in the Planktonic Diatom Ditylum brightwellii (Bacillariophyceae). Rynearson TA, Lin EO, Armbrust EV, Protist 2009 160 1 111 121 10.1016/j.protis.2008.10.003 19083268
87. Temperature and phytoplankton growth in the sea. Eppley RW, Fishery Bull (Washington DC) 1972 70 4 1063
88. The relationship between photosynthesis and light for natural assemblages of coastal marine plankton. Platt T, Jassby AD, J Phycol 1976 12 4 421 430
89. The estimation of phytoplankton production in the ocean from chlorophyll and light data. Ryther JH, Yentsch CS, Limnol Oceanogr 1957 2 3 281 286
90. Genetic variability in reprodution rates in marine phytoplankton populations. Brand LE, Evolution 1981 35 6 1117 1127 10.2307/2408125
91. The Problem of Environment and Selection. Falconer DS, Am Nat 1952 86 830 293 298 10.1086/281736
92. High levels of genetic diversity and low levels of genetic differentiation in North Sea Pseudo-nitzchia pungens (Bacillariophyceae) populations. Evans KM, J Phycol 2005 41 3 506 514 10.1111/j.1529-8817.2005. 00084.x (Pubitemid 40832009)
93. Intraspecific genetic diversity in the marine coccolithophore Emiliania huxleyi (Prymnesiophyceae): the use of microsatellite analysis in marine phytoplankton population studies. Iglesias-Rodriguez MD, Schofield OM, Batley J, Medlin LK, Hayes PK, J Phycol 2006 42 525 536
94. Hundred years of genetic structure in a sediment revived diatom population. Härnström K, Ellegaard M, Andersen TJ, Godhe A, Proc Natl Acad Sci 2011 108 10 4252 4257 10.1073/pnas.1013528108 21282612
95. Monterey Bay phytoplankton. II. Resting spore cycles in coastal diatom populations. Garrison DL, J Plankton Res 1981 3 1 137 156 10.1093/plankt/3.1.137
96. Temperature and seasonal occurence patterns of 30 dominant phytoplankton species in Narragansett Bay over a 22-year period (1969-1980.). Karentz D, Smayda TJ, Mar Ecol Prog Ser 1984 18 277 293
97. Importance of resting stages in diatom seasonal succession. McQuoid MR, Hobson LA, J Phycol 1995 31 1 44 50 10.1111/j.0022-3646.1995.00044.x (Pubitemid 26447730)
98. Diatom resting stages. McQuoid MR, Hobson LA, J Phycol 1996 32 6 889 902 10.1111/j.0022-3646.1996.00889.x (Pubitemid 27047800)
99. The species concept in diatoms. Mann DG, Phycologia 1999 38 6 437 495 10.2216/i0031-8884-38-6-437.1 (Pubitemid 30023338)