Enhancement of recombinant human EPO production and glycosylation in serum-free suspension culture of CHO cells through expression and supplementation of 30Kc19

Applied Microbiology and Biotechnology

Volumn 96, Issue 3, 2012, Pages 671-683

  Park, Ju Hyun ^a   Wang, Zesong ^a,b   Jeong, Hee Jin ^a   Park, Hee Ho ^a   Kim, Byung Gee ^a   Tan, Wen Song ^b   Choi, Shin Sik ^c   Park, Tai Hyun ^a  

^a Seoul National University   (South Korea)

^b EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^c Myong Ji University   (South Korea)

Author keywords

30Kc19 protein; CHO cells; Glycosylation; Productivity; rHuEPO

Indexed keywords

BOMBYX MORI; CHO CELL; CONTROL-CELL; CULTURE MEDIUM; GLYCAN STRUCTURES; GLYCANS; HOST CELLS; ISOFORMS; MALDI TOF MS; RECOMBINANT HUMAN EPO; RHUEPO; SIALIC ACIDS; SIALYLATION; SUSPENSION CULTURES; TWO-DIMENSIONAL ELECTROPHORESIS (2-DE); WESTERN-BLOT ANALYSIS;

BODY FLUIDS; CARBOXYLIC ACIDS; CELLS; ELECTROPHORESIS; ESCHERICHIA COLI; ESTERIFICATION; GENE EXPRESSION; GLYCOSYLATION; PRODUCTIVITY; RECOMBINANT PROTEINS;

CELL CULTURE;

30KC19 PROTEIN; FUCOSE; GLYCAN; RECOMBINANT ERYTHROPOIETIN; RECOMBINANT PROTEIN; SIALIC ACID; UNCLASSIFIED DRUG;

CYTOLOGY; GENE EXPRESSION; IMMUNOASSAY; PROTEIN; RECOMBINATION; SERUM;

ANIMAL CELL; ARTICLE; BOMBYX MORI; CARBOHYDRATE ANALYSIS; CELL SUSPENSION; CHO CELL; CONTROLLED STUDY; CULTURE MEDIUM; ESCHERICHIA COLI; FEMALE; GENE EXPRESSION; HORMONE SYNTHESIS; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PROTEIN EXPRESSION; PROTEIN GLYCOSYLATION; SIALYLATION; TWO DIMENSIONAL ELECTROPHORESIS; WESTERN BLOTTING;

ANIMALS; BLOTTING, WESTERN; BOMBYX; CHO CELLS; CRICETINAE; CRICETULUS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ERYTHROPOIETIN; GENE EXPRESSION; GLYCOSYLATION; HUMANS; INSECT PROTEINS; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

BOMBYX MORI; ESCHERICHIA COLI; TETRA;

EID: 84867574563     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-4203-0     Document Type: Article

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