Mass spectrometric quantification of neutral and sialylated N-glycans from a recombinant therapeutic glycoprotein produced in the two Chinese hamster ovary cell lines

Analytical Biochemistry

Volumn 386, Issue 2, 2009, Pages 228-236

  Jang, Kyoung Soon ^a   Kim, Yun Gon ^a   Gil, Geun Cheol ^a   Park, Sang Ho ^b   Kim, Byung Gee ^a  

^a Seoul National University   (South Korea)

^b Isu Abxis Co Ltd   (South Korea)

Author keywords

CHO cell; Girard's reagent T; MALDI TOF; Methyl esterification; Quantification; Sialylated glycan

Indexed keywords

BIOACTIVITY; CELL CULTURE; CELLS; ESTERS; GLYCOPROTEINS; INDUCTIVELY COUPLED PLASMA; MASS SPECTROMETRY; OLIGOSACCHARIDES; QUALITY CONTROL; REAGENTS;

CHO CELL; GIRARD'S REAGENT T; MALDI-TOF; METHYL ESTERIFICATION; QUANTIFICATION; SIALYLATED GLYCAN;

POLYSACCHARIDES;

GLYCAN DERIVATIVE; GLYCOPROTEIN; METHYL GROUP; OLIGOSACCHARIDE; REAGENT;

ANIMAL CELL; ARTICLE; BIOLOGICAL ACTIVITY; CELL LINE; CHO CELL; DERIVATIZATION; ESTERIFICATION; FEMALE; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS;

CRICETULUS GRISEUS;

EID: 59749083362     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.12.015     Document Type: Article

References (30)

1. Fussenegger M., Bailey J.E., Hauser H., and Mueller P.P. Genetic optimization of recombinant glycoprotein production by mammalian cells. Trends Biotechnol. 17 (1999) 35-42
2. Sethuraman N., and Stadheim T.A. Challenges in therapeutic glycoprotein production. Curr. Opin. Biotechnol. 17 (2006) 341-346
3. Roy S.K., Chiba Y., and Jigami Y. Production of therapeutic glycoproteins through the engineering of glycosylation pathway in yeast. Biotechnol. Bioprocess Eng. 5 (2000) 219-226
4. Jefferis R. Glycosylation of natural and recombinant antibody molecules. Adv. Exp. Med. Biol. 564 (2005) 143-148
5. Serrato J.A., Hernandez V., Estrada-Mondaca S., Palomares L.A., and Ramirez O.T. Differences in the glycosylation profile of a monoclonal antibody produced by hybridomas cultured in serum-supplemented, serum-free, or chemically defined media. Biotechnol. Appl. Biochem. 47 (2007) 113-124
6. Gawlitzek M., Ryll T., Lofgren J., and Sliwkowski M.B. Ammonium alters N-glycan structures of recombinant TNFR-IgG: degradative versus biosynthetic mechanisms. Biotechnol. Bioeng. 68 (2000) 637-646
7. Borys M.C., Linzer D.I., and Papoutsakis E.T. Culture pH affects expression rates and glycosylation of recombinant mouse placental lactogen proteins by Chinese hamster ovary (CHO) cells. Biotechnology 11 (1993) 720-724
8. James D.C., Freedman R.B., Hoare M., Ogonah O.W., Rooney B.C., Larionov O.A., Dobrovolsky V.N., Lagutin O.V., and Jenkins N. N-Glycosylation of recombinant human interferon-γ produced in different animal expression systems. Biotechnology 13 (1995) 592-596
9. Werner R.G., Kopp K., and Schlueter M. Glycosylation of therapeutic proteins in different production systems. Acta Paediatr. Suppl. 96 (2007) 17-22
10. Choi Y.S., Lee D.Y., Kim I.Y., Kim H.J., Park H.W., Choe T.B., and Kim I.H. Enhancement of erythropoietin production in recombinant Chinese hamster ovary cells by sodium lactate addition. Biotechnol. Bioprocess Eng. 12 (2007) 60-72
11. Stockert R.J. The asialoglycoprotein receptor: Relationships between structure, function, and expression. Physiol. Rev. 75 (1995) 591-609
12. Yuen C.T., Storring P.L., Tiplady R.J., Izquierdo M., Wait R., Gee C.K., Gerson P., Lloyd P., and Cremata J.A. Relationships between the N-glycan structures and biological activities of recombinant human erythropoietins produced using different culture conditions and purification procedures. Br. J. Haematol. 121 (2003) 511-526
13. 1-Fc: properties of a series of truncated glycoforms. Mol. Immunol. 37 (2000) 697-706
14. Toyoda T., Arakawa T., and Yamaguchi H. N-Glycans stabilize human erythropoietin through hydrophobic interactions with the hydrophobic protein surface. Studies by surface plasmon resonance analysis. J. Biochem. 131 (2002) 511-515
15. Barroso B., Didraga M., and Bischoff R. Analysis of proteoglycans derived sulphated disaccharides by liquid chromatography/mass spectrometry. J. Chromatogr. A 1080 (2005) 43-48
16. Bisio A., Mantegazza A., Urso E., Naggi A., Torri G., Viskov C., and Casu B. High-performance liquid chromatographic/mass spectrometric studies on the susceptibility of heparin species to cleavage by heparanase. Semin. Thromb. Hemost. 33 (2007) 488-495
17. Chaturvedi P., Warren C.D., Ruiz-Palacios G.M., Pickering L.K., and Newburg D.S. Milk oligosaccharide profiles by reversed-phase HPLC of their perbenzoylated derivatives. Anal. Biochem. 251 (1997) 89-97
18. Chen X., and Flynn G.C. Analysis of N-glycans from recombinant immunoglobulin G by on-line reversed-phase high-performance liquid chromatography/mass spectrometry. Anal. Biochem. 370 (2007) 147-161
19. Hitchcock A.M., Costello C.E., and Zaia J. Glycoform quantification of chondroitin/dermatan sulfate using a liquid chromatography-tandem mass spectrometry platform. Biochemistry 45 (2006) 2350-2361
20. Behr J.R., Matsumoto Y., White F.M., and Sasisekharan R. Quantification of isomers from a mixture of twelve heparin and heparan sulfate disaccharides using tandem mass spectrometry. Rapid Commun. Mass Spectrom. 19 (2005) 2553-2562
21. Ninonuevo M.R., Ward R.E., LoCascio R.G., German J.B., Freeman S.L., Barboza M., Mills D.A., and Lebrilla C.B. Methods for the quantitation of human milk oligosaccharides in bacterial fermentation by mass spectrometry. Anal. Biochem. 361 (2007) 15-23
22. Bowman M.J., and Zaia J. Tags for the stable isotopic labeling of carbohydrates and quantitative analysis by mass spectrometry. Anal. Chem. 79 (2007) 5777-5784
23. Gil G.C., Kim Y.G., and Kim B.G. A relative and absolute quantification of neutral N-linked oligosaccharides using modification with carboxymethyl trimethylammonium hydrazide and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Anal. Biochem. 379 (2008) 45-59
24. Gouw J.W., Burgers P.C., Trikoupis M.A., and Terlouw J.K. Derivatization of small oligosaccharides prior to analysis by matrix-assisted laser desorption/ionization using glycidyltrimethylammonium chloride and Girard's reagent T. Rapid Commun. Mass Spectrom. 16 (2002) 905-912
25. Naven T.J.P., and Harvey D.J. Cationic derivatization of oligosaccharides with Girard's T reagent for improved performance in matrix-assisted laser desorption/ionization and electrospray mass spectrometry. Rapid Commun. Mass Spectrom. 10 (1996) 829-834
26. Ciucanu I., and Kerek F. Rapid and simultaneous methylation of fatty acids and hydroxy FA for gas chromatography analysis. J. Chromatogr. 284 (1984) 179-185
27. Handa S., and Nakamura K. Modification of sialic acid carboxyl group of ganglioside. J. Biochem. 95 (1984) 1323-1329
28. Bigge J.C., Patel T.P., Bruce J.A., Goulding P.N., Charles S.M., and Parekh R.B. Nonselective and efficient fluorescent labeling of glycans using 2-amino benzamide and anthranilic acid. Anal. Biochem. 230 (1995) 229-238
29. Toyoda M., Ito H., Matsuno Y.K., Narimatsu H., and Kameyama A. Quantitative derivatization of sialic acids for the detection of sialoglycans by MALDI MS. Anal. Chem. 80 (2008) 5211-5218
30. Elliott S., Lorenzini T., Asher S., Aoki K., Brankow D., Buck L., Busse L., Chang D., Fuller J., Grant J., Hernday N., Hokum M., Hu S., Knudten A., Levin N., Komorowski R., Martin F., Navarro R., Osslund T., Rogers G., Rogers N., Trail G., and Egrie J. Enhancement of therapeutic protein in vivo activities through glycoengineering. Nat. Biotechnol. 21 (2003) 414-421