Three-dimensional architecture and gating mechanism of a K+ channel studied by EPR spectroscopy

Nature Structural Biology

Volumn 5, Issue 6, 1998, Pages 459-469

  Perozo, Eduardo ^a   Marien Cortes, D ^a   Cuello, Luis G ^a  

^a UNIVERSITY OF VIRGINIA HEALTH SYSTEM   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MONOMER; POTASSIUM CHANNEL; POTASSIUM ION; TETRAMER;

ALPHA HELIX; AMINO ACID SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CHANNEL GATING; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; ELECTRON SPIN RESONANCE; NONHUMAN; PRIORITY JOURNAL; PROTEIN LIPID INTERACTION; STREPTOMYCES LIVIDANS;

AMINO ACID SEQUENCE; ANIMALS; BACTERIAL PROTEINS; CRYSTALLOGRAPHY, X-RAY; CYSTEINE; ELECTRON SPIN RESONANCE SPECTROSCOPY; ION CHANNEL GATING; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PEPTIDE FRAGMENTS; POTASSIUM CHANNELS; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; SEQUENCE ALIGNMENT; SHAKER SUPERFAMILY OF POTASSIUM CHANNELS; STREPTOMYCES;

POSIBACTERIA; STREPTOMYCES LIVIDANS;

EID: 2642701712     PISSN: 10728368     EISSN: None     Source Type: Journal    
DOI: 10.1038/nsb0698-459     Document Type: Article

References (48)

1. Hille, B. Ion channels of excitable membranes, (Sinauer, Sunderland, Massachusetts; 1992).
2. Miller, C. 1990: annus mirabilis of potassium channels. Science 252, 1092-1096 (1991).
3. Jan, L.Y. & Jan, Y.N. Potassium channels and their evolving gates. Nature 371, 119-122 (1994).
4. Jan, L.Y. & Jan, Y.N. Cloned potassium channels from eukaryotes and prokaryotes. Annu. Rev. Neurosci. 20, 91-123 (1997).
5. Schrempf, H. et al. A prokaryotic potassium ion channel with two predicted transmembrane segments from Streptomyces lividans. EMBO J. 14, 5170-5178 (1995).
6. + channels. Science 258, 1152-1155 (1992).
7. + channel. Biochemistry 36, 10335-10342 (1997).
8. + channel (SKC1): Oligomeric stoichiometry and stability. Biochemistry 36, 10343-10352 (1997).
9. + channel (SKC1): Secondary structure characterization from FTIR spertroscopy. FEBS Lett. 423, 205-212 (1998).
10. + channel. Biochemistry 37, 3229-3236 (1998).
11. + conduction and selectivity. Science 280, 69-77 (1998).
12. Hubbell, W.L. & Altenbach, C. Site-directed spin labeling of membrane proteins, (Oxford University Press, New York; 1994).
13. Hubbell, W.L., McHaourab, H.S., Altenbach, C. & Lietzow, M.A. Watching proteins move using site-directed spin labeling. Structure 4, 779-783 (1996).
14. Falke, J.J. et al. Structure of a bacterial sensory receptor. A site-directed sulfhydryl study. J. Biol. Chem. 263, 14850-14858 (1988).
15. Rabenstein, M.D. & Shin, Y.K. Determination of the distance between two spin labels attached to a macromolecule. Proc. Natl. Acad. Sci. USA 92, 8239-8243 (1995).
16. Hustedt, E.J., Smirnov, A.I., Laub, C.F., Cobb, C.E. & Beth, A.H. Molecular distances from dipolar coupled spin-labels - the global analysis of multifrequency continuous wave electron paramagnetic resonance data. Biophys. J. 72, 1861-1877 (1997).
17. Mchaourab, H.S., Oh, K.J., Fang, C.J. & Hubbell, W.L. Conformation of T4 lysozyme in solution. Hinge-bending motion and the substrate-induced conformational transition studied by site-directed spin labeling. Biochemistry 36, 307-316 (1997).
18. Altenbach, C., Marti, T., Khorana, H.G. & Hubbell, W.L. Transmembrane protein structure: spin labeling of bacteriorhodopsin mutants. Science 248, 1088-1092 (1990).
19. Shin, Y.K., Levinthal, C., Levinthal, F. & Hubbell, W.L. Colicin E1 binding to membranes: time-resolved studies of spin-labeled mutants. Science 259, 960-963 (1993).
20. Steinhoff, H.J. et al. Time-resolved detection of structural changes during the photocycle of spin-labeled bacteriorhodopsin. Science 266, 105-107 (1994).
21. Oh, K.J. et al. Organization of diphtheria toxin T domain in bilayers: a site-directed spin labeling study. Science 273, 810-812 (1996).
22. Farrens, D.L., Altenbach, C., Yang, K., Hubbell, W.L. & Khorana, H.G. Requirement of rigid-body motion of transmembrane helices for light activation of rhodopsin. Science 274, 768-770 (1996).
23. Subczynski, W.K. & Hyde, J.S. The diffusion-concentration product of oxygen in lipid bilayers using the spin-label T1 method. Biochim. Biophys. Acta 643, 283-291 (1981).
24. Altenbach, C., Froncisz, W., Hyde, J.S. & Hubbell, W.L. Conformation of spin-labeled melittin at membrane surfaces investigated by pulse saturation recovery and continuous wave power saturation electron paramagnetic resonance. Biophys. J. 56, 1183-1191 (1989).
25. Mchaourab, H.S., Lietzow, M.A., Hideg, K. & Hubbell, W.L. Motion of spin-labeled side chains in T4 lysozyme. Correlation with protein structure and dynamics. Biochemistry 35, 7692-7704 (1996).
26. Eisenberg, D., Weiss, R.M. & Terwilliger, T.C. The hydrophobic moment detects periodicity in protein hydrophobicity. Proc. Natl. Acad. Sci. USA 81, 140-144 (1984).
27. Cornette, J.L. et al. Hydrophobicity scales and computational techniques for detecting amphipathic structures in proteins. J. Mol. Biol. 195, 659-685 (1987).
28. Altenbach, C., Greenhalgh, D.A., Khorana, H.G. & Hubbell, W.L. A collision gradient method to determine the immersion depth of nitroxides in lipid bilayers: application to spin-labeled mutants of bacteriorhodopsin. Proc. Natl. Acad. Sci. USA 91, 1667-1671 (1994).
29. Kulikov, A.V., Likhtenshtein, G.I., Rozantsev, E.G. & Suskina, V.I. Possibility of determining the distances between the functional groups of proteins by the method of paramagnetic labels. Biofizika 17, 42-48 (1972).
30. + channels: evidence for a trap door mechanism of activation gating. J. Gen. Phys. 109, 527-535 (1997).
31. + channel. Neuron 19, 175-184 (1997).
32. Walther, D., Eisenhaber, F. & Argos, P. Principles of helix-helix packing in proteins - the helical lattice superposition model. J. Mol. Biol. 255, 536-553 (1996).
33. Bowie, J.U. Helix packing angle preferences. Nature Struct. Biol. 4, 915-917 (1997).
34. Mingarro, I., Elofsson, A. & Vonheijne, G. Helix-helix packing in a membrane-like environment. J. Mol. Biol. 272, 633-641 (1997).
35. Connolly, M.L. Solvent-accessible surfaces of proteins and nucleic acids. Science 221, 709-713 (1983).
36. + channel comprises part of the pore. Nature 367, 179-182 (1994).
37. Choi, K.L., Mossman, C., Aube, J. & Yellen, G. The internal quaternary ammonium receptor site of Shaker potassium channels. Neuron 10, 533-541 (1993).
38. + channels. Biophys. J. 67, 2316-2325 (1994).
39. + channel. Nature 371, 243-246 (1994).
40. Armstrong, C.M. Time course of TEA+-induced anomalous rectification in squid giant axons. J. Gen. Phys. 50, 491-503 (1966).
41. Armstrong, C.M., Swenson, R.P., Jr. & Taylor, S.R. Block of squid axon K channels by internally and externally applied barium ions. J. Gen. Phys. 80, 663-682 (1982).
42. + channel. J. Gen. Phys. 90, 427-449 (1987).
43. Grissmer, S. & Cahalan, M.D. Divalent ion trapping inside potassium channels of human T lymphocytes. J. Gen. Phys. 93, 609-630 (1989).
44. Sun, Z.P., Akabas, M.H., Goulding, E.H., Karlin, A. & Siegelbaum, S.A. Exposure of residues in the cyclic nucleotide-gated channel pore: P region structure and function in gating. Neuron 16, 141-149 (1996).
45. Garty, H., Rudy, B. & Karlish, S.J. A simple and sensitive procedure for measuring isotope fluxes through ion-specific channels in heterogenous populations of membrane vesicles. J. Biol. Chem. 258, 13094-13099 (1983).
46. Becktel, W.J. & Schellman, J.A. Protein stability curves. Biopolymers 26, 1859-1877 (1987).
47. Farahbakhsh, Z.T., Altenbach, C. & Hubbell, W.L. Spin labeled cysteines as sensors for protein-lipid interaction and conformation in rhodopsin. Photochemistry & Photobiology 56, 1019-1033 (1992).
48. Donnelly, D., Overington, J.P. & Blundell, T.L. The prediction and orientation of alpha-helices from sequence alignments: the combined use of environment-dependent substitution tables, Fourier transform methods and helix capping rules. Prot. Engng. 7, 645-653 (1994).