The NMR Structure of Human Obestatin in Membrane-Like Environments: Insights into the Structure-Bioactivity Relationship of Obestatin

PLoS ONE

Volumn 7, Issue 10, 2012, Pages

  Alén, Begoña O ^a,b,c   Nieto, Lidia ^d,e   Gurriarán Rodríguez, Uxía ^a,b,c   Mosteiro, Carlos S ^a,b   Álvarez Pérez, Juan C ^a,b,c,f   Otero Alén, María ^a,g   Camiña, Jesús P ^a,b   Gallego, Rosalía ^a,c   García Caballero, Tomás ^a,c   Martín Pastor, Manuel ^a,c   Casanueva, Felipe F ^a,b,c   Jiménez Barbero, Jesús ^d   Pazos, Yolanda ^a,b  

^a HOSPITAL CLÍNICO UNIVERSITARIO   (Spain)

^b INSTITUTO DE SALUD CARLOS III   (Spain)

^c UNIVERSITY OF SANTIAGO DE COMPOSTELA   (Spain)

^d CENTRO DE INVESTIGACIONES BIOLÓGICAS   (Spain)

^e EINDHOVEN UNIVERSITY OF TECHNOLOGY   (Netherlands)

^f UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^g Nodo Central Biobanco de Tumores de Andalucía   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

DODECYL SULFATE SODIUM; KI 67 ANTIGEN; MITOGEN ACTIVATED PROTEIN KINASE 1; NONAMIDATED OBESTATIN; OBESTATIN; OBESTATIN[11-23]; OBESTATIN[16-23]; OBESTATIN[6-23]; PROTEIN KINASE B; UNCLASSIFIED DRUG;

ALPHA HELIX; ANTIGEN EXPRESSION; ARTICLE; CELL PROLIFERATION; CIRCULAR DICHROISM; CONTROLLED STUDY; ENZYME PHOSPHORYLATION; HORMONE ACTION; HORMONE STRUCTURE; HUMAN; HUMAN CELL; IN VITRO STUDY; MICELLE; MOLECULAR MODEL; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PIGMENT EPITHELIUM; SPECIES DIFFERENCE; STRUCTURE ACTIVITY RELATION;

AMINO ACID SEQUENCE; ANIMALS; CELL LINE; CELL MEMBRANE; CELL PROLIFERATION; CIRCULAR DICHROISM; EPITHELIAL CELLS; GHRELIN; HUMANS; IMMUNOHISTOCHEMISTRY; KI-67 ANTIGEN; MAGNETIC RESONANCE SPECTROSCOPY; MICE; MICELLES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; RECEPTORS, G-PROTEIN-COUPLED; RETINAL PIGMENT EPITHELIUM; SEQUENCE HOMOLOGY, AMINO ACID; SODIUM DODECYL SULFATE; SOLUTIONS; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 84867135520     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0045434     Document Type: Article

References (30)

1. Zhang JV, Re PG, Avsian-Kretchmer O, Luo CW, Rauch R, et al. (2005) Obestatin, a peptide encoded by the ghrelin gene, opposes ghrelin's effects on food intake. Science 310: 996-999.
2. Samson WK, White MM, Price C, Ferguson AV, (2007) Obestatin acts in brain to inhibit thirst. Am J Physiol-Regul Integr Comp Physiol 292: R637-R643.
3. Zhang JV, Jahr H, Luo CW, Klein C, Van Kolen K, et al. (2008) Obestatin induction of early response gene expression in gastrointestinal and adipose tissues and the mediatory role of GPR39. Mol Endocrinol 22: 1464-1475.
4. Seim I, Walpole C, Amorim L, Josh P, Herington A, et al. (2011) The expanding roles of the ghrelin-gene derived peptide obestatin in health and disease. Mol Cell Endocrinol 340: 111-117.
5. Camiña JP, Campos JF, Caminos JE, Diéguez C, Casanueva FF, (2007) Obestatin-mediated proliferation of human retinal pigment epitelial cells: regulatory mechanisms. J Cell Physiol 211: 1-9.
6. Pazos Y, Alvarez CJ, Camiña JP, Casanueva FF, (2007) Stimulation of extracellular signal-regulated kinases and proliferation in the human gastric cancer cells KATO-III by obestatin. Growth Factors 25: 373-381.
7. Alvarez CJ, Lodeiro M, Theodoropoulou M, Camiña JP, Casanueva FF, et al. (2009) Obestatin stimulates Akt signalling in gastric cancer cells through β-arrestin-mediated epidermal growth factor receptor transactivation. Endocr-Relat Cancer 16: 599-611.
8. Plotnikov A, Zehorai E, Procaccia S, Seger R, (2011) The MAPK cascades: signaling components, nuclear roles and mechanisms of nuclear translocation. Biochim Biophys Acta 1813: 1619-33.
9. Manning BD, Cantley LC, (2007) AKT/PKB signaling: navigating downstream. Cell 129: 1261-1274.
10. Gurriarán-Rodríguez U, Al-Massadi O, Roca-Rivada A, Crujeiras AB, Gallego R, et al. (2011) Obestatin as a regulator of adipocyte metabolism and adipogenesis. J Cell Mol Med 15: 1927-1940.
11. Gurriarán-Rodríguez U, Al-Massadi O, Crujeiras AB, Mosteiro CS, Amil-Diz M, et al. (2011) Preproghrelin expression is a key target for insulin action on adipogenesis. J Endocrinol 210: R1-R7.
12. Nagaraj S, Peddha MS, Manjappara UV, (2008) Fragments of obestatin as modulators of feed intake, circulating lipids, and stored fat. Biochem Biophys Res Commun 366: 731-737.
13. Scrima M, Campiglia P, Esposito C, Gomez-Monterrey I, Novellino E, et al. (2007) Obestatin conformational features: a strategy to unveil obestatin's biological role? Biochem Biophys Res Commun 363: 500-505.
14. Subasinghage AP, Green BD, Flatt PR, Irwin N, Hewage CM, (2010) Metabolic and structural properties of human obestatin {1-23} and two fragment peptides. Peptides 31: 1697-1705.
15. Schluter C, Duchrow M, Wohlenberg C, Becker MH, Key G, et al. (1993) The cell proliferation-associated antigen of antibody Ki67: a very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell-cycle-maintaining proteins. J Cell Biol 123: 513-522.
16. Keller RLJ. (2004) The Computer Aided Resonance Assignment tutorial. Goldau: CANTINA Verlag. CARA website. Available: http://cara.nmr-software.org/portal/. Accessed 2012 Sep 5.
17. Güntert P, (2004) Automated NMR structure calculation with CYANA. Methods Mol Biol 278: 353-378.
18. Case DA, Cheatham TE 3rd, Darden T, Gohlke H, Luo R, et al (2005) The Amber biomolecular simulation programs. J Comput Chem 26: 1668-1688.
19. Koradi R, Billeter M, Wüthrich K, (1996) MOLMOL: a program for display and analysis of macromolecular structures. J Mol Graph 14: 51-54.
20. Laskowski RA, Rullmannn JA, MacArthur MW, Kaptein R, Thornton JM, (1996) AQUA and PROCHECK-NMR: programs for checking the quality of protein structures solved by NMR. J Biomol NMR 8: 477-486.
21. Kelly SM, Jess TJ, Price NC, (2005) How to study proteins by circular dichroism. Biochim Biophys Acta 1751: 119-139.
22. Whitmore L, Wallace BA, (2004) DICHROWEB: an online server for protein secondary structure analyses from circular dichroism spectroscopic data. Nucleic Acids Res 32: W668-W673.
23. Whitmore L, Wallace BA, (2008) Protein Secondary Structure Analyses from Circular Dichroism Spectroscopy: Methods and Reference Databases. Biopolymers 89: 392-400.
24. Greenfield NJ, (2006) Using circular dichroism spectra to estimate protein secondary structure. Nat Protoc 1: 2876-2890.
25. Schwyzer R, (1995) In search of the "bio-active conformation" - is it induced by the target cell membrane? J Mol Recognit 8: 3-8.
26. Berjanskii VM, Wishart DS, (2005) A Simple Method To Predict Protein Flexibility Using Secondary Chemical Shifts. J Am Chem Soc 127: 14970-14971.
27. Whalen EJ, Rajagopal S, Lefkowitz RJ, (2011) Therapeutic potential of β-arrestin- and G-protein-biased agonists. Trends Mol Med 17: 126-139.
28. Reiter E, Ahn S, Shukla AK, Lefkowitz RJ, (2012) Molecular mechanism of β-arrestin-biased agonism at seven-transmembrane receptors. Annu Rev Pharmacol Toxicol 52: 179-197.
29. Yerushalmi R, Woods R, Ravdin PM, Hayes MM, Gelmon KA, (2010) Ki67 in breast cancer: prognostic and predictive potential. Lancet Oncol 11: 174-183.
30. Gerdes J, Schwab U, Lemke H, Stein H, (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer 31: 13-20.