Measurement of AMP-activated protein kinase activity and expression in response to ghrelin

Methods in Enzymology

Volumn 514, Issue , 2012, Pages 271-287

  Lim, Chung Thong ^a   Lolli, Francesca ^a   Thomas, Julia D ^a   Kola, Blerina ^a   Korbonits, Márta ^a  

^a QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

Author keywords

AMP activated protein kinase; AMPK assay; enzyme study; gene expression; ghrelin; immunoblotting; immunoprecipitation; Western blot

Indexed keywords

ACETYL COENZYME A CARBOXYLASE; ADENOSINE TRIPHOSPHATE; COMPLEMENTARY DNA; GHRELIN; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE KINASE; MESSENGER RNA; RNA;

ARTICLE; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME PHOSPHORYLATION; ENZYME SUBSTRATE; GENE EXPRESSION; IMMUNOBLOTTING; IMMUNOPRECIPITATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; WESTERN BLOTTING;

EID: 84866251556     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-381272-8.00017-9     Document Type: Chapter

References (46)

1. K.A. Anderson Hypothalamic CaMKK2 contributes to the regulation of energy balance Cell Metab. 7 2008 377 388
2. U. Andersson AMP-activated protein kinase plays a role in the control of food intake J. Biol. Chem. 279 2004 12005 12008
3. Z.B. Andrews UCP2 mediates ghrelin's action on NPY/AgRP neurons by lowering free radicals Nature 454 2008 846 851
4. Z.B. Andrews Uncoupling protein-2 decreases the lipogenic actions of ghrelin Endocrinology 151 2010 2078 2086
5. C. Cao Ghrelin inhibits insulin resistance induced by glucotoxicity and lipotoxicity in cardiomyocyte Peptides 32 2011 209 215
6. D. Carling A common bicyclic protein kinase cascade inactivates the regulatory enzymes of fatty acid and cholesterol biosynthesis FEBS Lett. 223 1987 217 222
7. D. Carling Purification and characterization of the AMP-activated protein kinase. Copurification of acetyl-CoA carboxylase kinase and 3-hydroxy-3-methylglutaryl-CoA reductase kinase activities Eur. J. Biochem. 186 1989 129 136
8. T.R. Castaneda Ghrelin in the regulation of body weight and metabolism Front. Neuroendocrinol. 31 2010 44 60
9. H.Y. Chen Orexigenic action of peripheral ghrelin is mediated by neuropeptide Y and agouti-related protein Endocrinology 145 2004 2607 2612
10. J.H. Chen Ghrelin induces cell migration through GHS-R, CaMKII, AMPK, and NF-kappaB signaling pathway in glioma cells J. Cell. Biochem. 112 2011 2931 2941
11. M. Christ-Crain AMP-activated protein kinase mediates glucocorticoid-induced metabolic changes: a novel mechanism in Cushing's syndrome FASEB J. 22 2008 1672 1683
12. S.P. Davies Tissue distribution of the AMP-activated protein kinase, and lack of activation by cyclic-AMP-dependent protein kinase, studied using a specific and sensitive peptide assay Eur. J. Biochem. 186 1989 123 128
13. N. Fukushima Ghrelin directly regulates bone formation J. Bone Miner. Res. 20 2005 790 798
14. D.G. Hardie AMP-activated protein kinase - an energy sensor that regulates all aspects of cell function Genes Dev. 25 2011 1895 1908
15. D.G. Hardie AMP-activated protein kinase: an ultrasensitive system for monitoring cellular energy charge Biochem. J. 338 Pt. 3 1999 717 722
16. D.G. Hardie AMP-activated protein kinase: also regulated by ADP? Trends Biochem. Sci. 36 2011 470 477
17. J.E. Healy Peripheral ghrelin stimulates feeding behavior and positive energy balance in a sciurid hibernator Horm. Behav. 59 2011 512 519
18. i via AMPK in hypothalamic arcuate nucleus NPY neurons Biochem. Biophys. Res. Commun. 366 2008 388 392
19. M. Kojima Ghrelin is a growth-hormone-releasing acylated peptide from stomach Nature 402 1999 656 660
20. B. Kola Role of AMP-activated protein kinase in the control of appetite J. Neuroendocrinol. 20 2008 942 951
21. B. Kola Cannabinoids and ghrelin have both central and peripheral metabolic and cardiac effects via AMP-activated protein kinase J. Biol. Chem. 280 2005 25196 25201
22. B. Kola Expanding role of AMPK in endocrinology Trends Endocrinol. Metab. 17 2006 205 215
23. B. Kola The orexigenic effect of ghrelin is mediated through central activation of the endogenous cannabinoid system PLoS One 3 2008 e1797
24. B. Kola The role of AMP-activated protein kinase in obesity Front. Horm. Res. 36 2008 198 211
25. M. Korbonits Ghrelin - a hormone with multiple functions Front. Neuroendocrinol. 25 2004 27 68
26. R. Lage Ghrelin effects on neuropeptides in the rat hypothalamus depend on fatty acid metabolism actions on BSX but not on gender FASEB J. 24 2011 2670 2679
27. A.F. Leite-Moreira, and J.B. Soares Physiological, pathological and potential therapeutic roles of ghrelin Drug Discov. Today 12 2007 276 288
28. C.T. Lim The ghrelin/GOAT/GHS-R system and energy metabolism Rev. Endocr. Metab. Disord. 12 2011 173 186
29. M. Lopez Hypothalamic fatty acid metabolism mediates the orexigenic action of ghrelin Cell Metab. 7 2008 389 399
30. Y. Minokoshi AMP-kinase regulates food intake by responding to hormonal and nutrient signals in the hypothalamus Nature 428 2004 569 574
31. J. Ning, G. Xi, and D.R. Clemmons Suppression of AMPK activation via S485 phosphorylation by IGF-I during hyperglycemia is mediated by AKT activation in vascular smooth muscle cells Endocrinology 152 2011 3143 3154
32. C. Rigault Ghrelin reduces hepatic mitochondrial fatty acid beta oxidation J. Endocrinol. Invest. 30 2007 RC4 RC8
33. S. Sangiao-Alvarellos Central ghrelin regulates peripheral lipid metabolism in a growth hormone-independent fashion Endocrinology 150 2009 4562 4574
34. S. Sangiao-Alvarellos Influence of ghrelin and growth hormone deficiency on AMP-activated protein kinase and hypothalamic lipid metabolism J. Neuroendocrinol. 22 2011 543 556
35. M.W. Sleeman, and E. Latres The CAMplexities of central ghrelin Cell Metab. 7 2008 361 362
36. P.K. Smith Measurement of protein using bicinchoninic acid Anal. Biochem. 150 1985 76 85
37. D. Stevanovic Immunomodulatory actions of central ghrelin in diet-induced energy imbalance Brain Behav. Immun. 26 2012 150 158
38. M. Suter Dissecting the role of 5′-AMP for allosteric stimulation, activation, and deactivation of AMP-activated protein kinase J. Biol. Chem. 281 2006 32207 32216
39. M. Tschop Ghrelin induces adiposity in rodents Nature 407 2000 908 913
40. A.J. Van Der Lely Biological, physiological, pathophysiological, and pharmacological aspects of ghrelin Endocr. Rev. 25 2004 426 457
41. H. Van Thuijl Appetite and metabolic effects of ghrelin and cannabinoids: involvement of AMP-activated protein kinase Vitam. Horm. 77 2008 121 148
42. W. Wang Ghrelin inhibits cell apoptosis induced by lipotoxicity in pancreatic beta-cell line Regul. Pept. 161 2010 43 50
43. A.M. Wren The novel hypothalamic peptide ghrelin stimulates food intake and growth hormone secretion Endocrinology 141 2000 4325 4328
44. A.M. Wren Ghrelin enhances appetite and increases food intake in humans J. Clin. Endocrinol. Metab. 86 2001 5992
45. X. Xu Molecular mechanisms of ghrelin-mediated endothelial nitric oxide synthase activation Endocrinology 149 2008 4183 4192
46. P. Xu Genetic selection for body weight in chickens has altered responses of the brain's AMPK system to food intake regulation effect of ghrelin, but not obestatin Behav. Brain Res. 221 2011 216 226