Cloning, characterization and expression analysis of δ 1-pyrroline-5-carboxylate synthetase (P5CS) gene in harvested papaya (Carica papaya) fruit under temperature stress

Food Research International

Volumn 49, Issue 1, 2012, Pages 272-279

  Zhu, Xiaoyang ^a   Li, Xueping ^a   Zou, Yuan ^a   Chen, Weixin ^a   Lu, Wangjin ^a  

^a SOUTH CHINA AGRICULTURAL UNIVERSITY   (China)

Author keywords

In silico cloning; P5CS; Papaya; Postharvest storage; Proline accumulation; Stress

Indexed keywords

IN-SILICO; P5CS; PAPAYA; POST-HARVEST STORAGE; PROLINE ACCUMULATION;

AMINO ACIDS; BIOCHEMISTRY; POLYMERASE CHAIN REACTION; STRESSES;

CLONING;

CARICA PAPAYA;

EID: 84866144288     PISSN: 09639969     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.foodres.2012.08.003     Document Type: Article

References (55)

1. An J.F., Paull R.E. Storage temperature and ethylene influence on ripening of papaya fruit. Journal of the American Society for Horticultural Science 1990, 115(6):949-953.
2. Andersen C.L., Jensen J.L., Orntoft T.F. Normalization of real-time quantitative reverse transcription-PCR data: A model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res 2004, 64(15):5245-5250.
3. Armengaud P., Thiery L., Buhot N., Grenier De March G., Savouré A. Transcriptional regulation of proline biosynthesis in Medicago truncatula reveals developmental and environmental specific features. Physiologia Plantarum 2004, 120(3):442-450.
4. Ashraf M., Foolad M.R. Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany 2007, 59(2):206-216.
5. Atienza S.G., Faccioli P., Perrotta G., Dalfino G., Zschiesche W., Humbeck K., et al. Large scale analysis of transcripts abundance in barley subjected to several single and combined abiotic stress conditions. Plant Science 2004, 167(6):1359-1365.
6. Badillo V.M. Carica L. vs. Vasconcella St. Hil. (Caricaceae) con la rehabilitación de este último. Ernstia 2000, 10(2):74-79.
7. Blas A.L., Ming R., Liu Z., Veatch O.J., Paull R.E., Moore P.H., et al. Cloning of the papaya chromoplast-specific lycopene β-cyclase, CpCYC-b, controlling fruit flesh color reveals conserved microsynteny and a recombination hot spot. Plant Physiology 2010, 152(4):2013-2022.
8. Bordoli L., Kiefer F., Arnold K., Benkert P., Battey J., Schwede T. Protein structure homology modeling using SWISS-MODEL workspace. Nature Protocols 2008, 4(1):1-13.
9. Chen Y., Lee Y., Yang C., Wang Y., Yang S., Shaw J. A novel papaya ACC oxidase gene (CP-ACO2) associated with late stage fruit ripening and leaf senescence. Plant Science 2003, 164(4):531-540.
10. Chen J.B., Wang S.M., Jing R.L., Mao X.G. Cloning the PvP5CS gene from common bean (Phaseolus vulgaris) and its expression patterns under abiotic stresses. Journal of Plant Physiology 2009, 166(1):12-19.
11. Chen J., Zhang X., Jing R., Blair M.W., Mao X., Wang S. Cloning and genetic diversity analysis of a new P5CS gene from common bean (Phaseolus vulgaris L.). TAG Theoretical and Applied Genetics 2010, 120(7):1393-1404.
12. Chen L., Zhong H., Kuang J., Li J., Lu W., Chen J. Validation of reference genes for RT-qPCR studies of gene expression in banana fruit under different experimental conditions. Planta 2011, 234(2):377.
13. Chiang H.H., Dandekar A.M. Regulation of proline accumulation in Arabidopsis thaliana (L.) Heynh during development and in response to desiccation. Plant, Cell & Environment 1995, 18(11):1280-1290.
14. Csonka L.N. Proline over-production results in enhanced osmotolerance in Salmonella typhimurium. Molecular and General Genetics MGG 1981, 182(1):82-86.
15. De Oliveira J.G., Vitoria A.P. Papaya: Nutritional and pharmacological characterization, and quality loss due to physiological disorders. An overview. Food Research International. 2011, 44(5):1306-1313.
16. Delauney A.J., Verma D.P.S. Proline biosynthesis and osmoregulation in plants. The Plant Journal 1993, 4(2):215-223.
17. Fernandes F.A.N., Rodrigues S., Gaspareto O.C.P., Oliveira E.L. Optimization of osmotic dehydration of papaya followed by air-drying. Food Research International 2006, 39(4):492-498.
18. 1-pyrroline-5-carboxylate synthetase from tomato. Plant Physiology 1998, 118(2):661-674.
19. 1-pyrroline-5-carboxylate synthase in alfalfa, transcriptionally induced upon salt stress. Plant Molecular Biology 1998, 38(5):755-764.
20. Hare P.D., Cress W.A. Metabolic implications of stress-induced proline accumulation in plants. Plant Growth Regulation 1997, 21(2):79-102.
21. Hare P.D., Cress W.A., Van Staden J. Proline synthesis and degradation: A model system for elucidating stress-related signal transduction. Journal of Experimental Botany 1999, 50(333):413-434.
22. 1-pyrroline-5-carboxylate synthetase results in increased proline accumulation and protection of plants from osmotic stress. Plant Physiology 2000, 122(4):1129-1136.
23. Hsu S.Y., Hsu Y.T., Kao C.H. The effect of polyethylene glycol on proline accumulation in rice leaves. Biologia Plantarum 2003, 46(1):73-78.
24. Hu C.A., Delauney A.J., Verma D.P. A bifunctional enzyme (delta 1-pyrroline-5-carboxylate synthetase) catalyzes the first two steps in proline biosynthesis in plants. Proceedings of the National Academy of Sciences 1992, 89(19):9354.
25. Hua X.J., van de Cotte B., Van Montagu M., Verbruggen N. Developmental regulation of pyrroline-5-carboxylate reductase gene expression in Arabidopsis. Plant Physiology 1997, 114(4):1215-1224.
26. Kishor P.B.K., Hong Z., Miao G.H., Hu C.A.A., Verma D.P.S. Overexpression of [delta]-pyrroline-5-carboxylate synthetase increases proline production and confers osmotolerance in transgenic plants. Plant Physiology 1995, 108(4):1387-1394.
27. Kishor P.B.K., Sangam S., Amrutha R.N., Laxmi P.S., Naidu K.R., Rao K., et al. Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: Its implications in plant growth and abiotic stress tolerance. Current Science 2005, 88(3):424-438.
28. Lehmann S., Funck D., Szabados L., Rentsch D. Proline metabolism and transport in plant development. Amino Acids 2010, 39(4):949-962.
29. Livak K.J., Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-[Delta][Delta] CT method. Methods 2001, 25(4):402-408.
30. Magné C., Larher F. High sugar content of extracts interferes with colorimetric determination of amino acids and free proline. Analytical Biochemistry 1992, 200(1):115-118.
31. Miller W.R., McDonald R.E. Irradiation, stage of maturity at harvest, and storage temperature during ripening affect papaya fruit quality. HortScience 1999, 34(6):1112-1115.
32. Mitra S.K. Postharvest physiology and storage of tropical and subtropical fruits 1997, Cab International.
33. Nanjo T., Fujita M., Seki M., Kato T., Tabata S., Shinozaki K. Toxicity of free proline revealed in an Arabidopsis T-DNA-tagged mutant deficient in proline dehydrogenase. Plant and Cell Physiology 2003, 44(5):541-548.
34. Nanjo T., Kobayashi M., Yoshiba Y., Sanada Y., Wada K., Tsukaya H., et al. Biological functions of proline in morphogenesis and osmotolerance revealed in antisense transgenic Arabidopsis thaliana. The Plant Journal 1999, 18(2):185-193.
35. Öztürk L., Demir Y. In vivo and in vitro protective role of proline. Plant Growth Regulation 2002, 38(3):259-264.
36. Paull R.E., Nishijima W., Reyes M., Cavaletto C. Postharvest handling and losses during marketing of papaya (Carica papaya). Postharvest Biology and Technology 1997, 11(3):165-179.
37. 1-pyrroline-5-carboxylate synthetase and proline dehydrogenase genes controls proline levels during and after osmotic stress in plants. Molecular and General Genetics MGG 1996, 253(3):334-341.
38. Roosens N.H.C.J., Thu T.T., Iskandar H.M., Jacobs M. Isolation of the ornithine-δ-aminotransferase cDNA and effect of salt stress on its expression in Arabidopsis thaliana. Plant Physiology 1998, 117(1):263-271.
39. Saradhi P.P., AliaArora S., Prasad K. Proline accumulates in plants exposed to UV radiation and protects them against UV-induced peroxidation. Biochemical and Biophysical Research Communications 1995, 209(1):1-5.
40. Savouré A., Jaoua S., Hua X.J., Ardiles W., Van Montagu M., Verbruggen N. Isolation, characterization, and chromosomal location of a gene encoding the [Delta] 1-pyrroline-5-carboxylate synthetase in Arabidopsis thaliana. FEBS letters 1995, 372(1):13-19.
41. Shiga T.M., Fabi J.P., Do Nascimento J.R.O., Petkowicz C.L.O., Vriesmann L.C., Lajolo F.M., et al. Changes in cell wall composition associated to the softening of ripening papaya: Evidence of extensive solubilization of large molecular mass galactouronides. Journal of Agricultural and Food Chemistry 2009, 57(15):7064-7071.
42. Siripornadulsil S., Traina S., Verma D.P.S., Sayre R.T. Molecular mechanisms of proline-mediated tolerance to toxic heavy metals in transgenic microalgae. The Plant Cell Online 2002, 14(11):2837-2847.
43. Strizhov N., Abraham E., Ökrész L., Blickling S., Zilberstein A., Schell J., et al. Differential expression of two P5CS genes controlling proline accumulation during salt-stress requires ABA and is regulated by ABA1, ABI1 and AXR2 in Arabidopsis. The Plant Journal 1997, 12(3):557-569.
44. Su M., Li X.F., Ma X.Y., Peng X.J., Zhao A.G., Cheng L.Q., et al. Cloning two P5CS genes from bioenergy sorghum and their expression profiles under abiotic stresses and MeJA treatment. Plant Science 2011, 181(6):652-659.
45. Székely G., Ábrahám E., Cséplo Á., Rigó G., Zsigmond L., Csiszár J., et al. Duplicated P5CS genes of Arabidopsis play distinct roles in stress regulation and developmental control of proline biosynthesis. The Plant Journal 2008, 53(1):11-28.
46. Udvardi M.K., Czechowski T., Scheible W.R. Eleven golden rules of quantitative RT-PCR. Plant Cell 2008, 20(7):1736-1737.
47. Vandesompele J., De Preter K., Pattyn F., Poppe B., Van Roy N., De Paepe A., et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biology 2002, 3(7):1.
48. Verbruggen N., Hermans C. Proline accumulation in plants: A review. Amino Acids 2008, 35(4):753-759.
49. Verbruggen N., Villarroel R., Van Montagu M. Osmoregulation of a pyrroline-5-carboxylate reductase gene in Arabidopsis thaliana. Plant Physiology 1993, 103(3):771-781.
50. Verslues P.E., Agarwal M., Katiyar Agarwal S., Zhu J., Zhu J.K. Methods and concepts in quantifying resistance to drought, salt and freezing, abiotic stresses that affect plant water status. The Plant Journal 2006, 45(4):523-539.
51. Wan C.Y., Wilkins T.A. A modified hot borate method significantly enhances the yield of high-quality RNA from cotton (Gossypium hirsutum L.). Analytical Biochemistry 1994, 223(1):7-12.
52. Wang W.G., Li R., Liu B., Li L., Wang S.H., Chen F. Effects of low nitrogen and drought stresses on proline synthesis of Jatropha curcas seedling. Acta Physiologiae Plantarum 2011, 33(5):1591-1595.
53. Yoshiba Y., Kiyosue T., Katagiri T., Ueda H., Mizoguchi T., Yamaguchi Shinozaki K., et al. Correlation between the induction of a gene for δ1-pyrroline-5-carboxylate synthetase and the accumulation of proline in Arabidopsis thaliana under osmotic stress. The Plant Journal 1995, 7(5):751-760.
54. Zhu J.K. Salt and drought stress signal transduction in plants. Annual Review of Plant Biology 2002, 53:247.
55. Zhu, X.Y., Li, X. P., Chen, W. X., Chen, J. Y., Lu, W. J., Chen, L., & Fu, D. W. (in press). Evaluation of New Reference Genes in Papaya for Accurate Transcript Normalization under Different Experimental Conditions. PLOS ONE, http://dx.doi.org/10.1371/journal.pone.0044405.