Icariin promotes extracellular matrix synthesis and gene expression of chondrocytes in vitro

Phytotherapy Research

Volumn 26, Issue 9, 2012, Pages 1385-1392

  Zhang, Lei ^a,b   Zhang, Xuan ^a   Li, Kui Feng ^a   Li, Dong Xiao ^b   Xiao, Yu Mei ^a   Fan, Yu Jiang ^a   Zhang, Xing Dong ^a  

^a SICHUAN UNIVERSITY   (China)

^b Sichuan Academy of Chinese Medicine Sciences   (China)

Author keywords

chondrocyte; extracellular matrix; gene expression; icariin; tissue engineering

Indexed keywords

AGGRECAN; BIOMATERIAL; COLLAGEN; COLLAGEN TYPE 1; COLLAGEN TYPE 2; GLYCOSAMINOGLYCAN; ICARIIN; TRANSCRIPTION FACTOR SOX9;

ANIMAL CELL; ARTICLE; ARTICULAR CARTILAGE; BIOSYNTHESIS; CARTILAGE CELL; CELL CULTURE; CELL ISOLATION; CELL PROLIFERATION; CELL STRUCTURE; CHONDROGENESIS; COLLAGEN SYNTHESIS; CONCENTRATION RESPONSE; CONTROLLED STUDY; DOWN REGULATION; DRUG CYTOTOXICITY; DRUG EFFECT; EPIMEDIUM; EXTRACELLULAR MATRIX; EXTRACELLULAR MATRIX SYNTHESIS; GENE EXPRESSION REGULATION; GLYCOSAMINOGLYCAN METABOLISM; IN VITRO STUDY; NONHUMAN; RABBIT; TISSUE ENGINEERING; UPREGULATION;

AGGRECANS; ANIMALS; CARTILAGE, ARTICULAR; CELL PROLIFERATION; CELLS, CULTURED; CHONDROCYTES; CHONDROGENESIS; COLLAGEN TYPE I; COLLAGEN TYPE II; EXTRACELLULAR MATRIX; FLAVONOIDS; GENE EXPRESSION REGULATION; GLYCOSAMINOGLYCANS; RABBITS; SOX9 TRANSCRIPTION FACTOR; TISSUE ENGINEERING;

EPIMEDIUM; ORYCTOLAGUS CUNICULUS;

EID: 84866136173     PISSN: 0951418X     EISSN: 10991573     Source Type: Journal    
DOI: 10.1002/ptr.3733     Document Type: Article

References (36)

1. Bosnakovski D, Mizuno M, Kim G, Takagi S, Okumura M, Fujinaga T,. 2006. Chondrogenic differentiation of bovine bone marrow mesenchymal stem cells (MSCs) in different hydrogels: influence of collagen type II extracellular matrix on MSC chondrogenesis. Biotechnol Bioeng 93 (6): 1152-1163.
2. Chen KM, Ge BF, Ma HP, Liu XY, Bai MH, Wang Y,. 2005. Icariin, a flavonoid from the herb Epimedium enhances the osteogenic differentiation of rat primary bone marrow stromal cells. Pharmazie 60: 939-942.
3. Chen YL, Lee HP, Chan HY, Sung LY, Chen HC, Hu YC,. 2007. Composite chondroitin-6-sulfate/dermatan sulfate/chitosan scaffolds for cartilage tissue engineering. Biomaterials 28: 2294-2305.
4. Chen YL, Chen HC, Chan HY, Chuang CK, Chang YH, Hu YC,. 2008. Co-conjugating chondroitin-6-sulfate/dermatan sulfate to chitosan scaffold alters chondrocyte gene expression and signaling profiles. Biotech Bioeng 101 (4): 821-830.
5. Chimal-Monroy J, Rodriguez-Leon J, Montero JA, et al., 2003. Analysis of the molecular cascade responsible for mesodermal limb chondrogenesis: Sox genes and BMP signaling. Dev Biol 257 (2): 292-301.
6. Coutts RD, Sah RL, Amiel D,. 1997. Effect of growth factors on cartilage repair. AAOS Inst Course Lect 46: 487-494.
7. Farndale RW, Buttle DJ, Barrett AJ,. 1986. Improved quantification and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue. Biochim Biophys Acta 883: 173-177.
8. Feng K, Xie W, Chen BL, et al., 2006. Effect of five species of Epimedium on growth of cartilage and proliferation of cartilage cell in vitro. China J Chin Materia Medica 31 (24): 2065-2067.
9. Feng K, Xie W, Chen BL, Ling CY,. 2009. Promoting effect of Epimedium on cartilage growth in vitro. J Clin Rehab 13 (15): 2823-2827.
10. Frenkel SR, Clancy RM, Ricci JL, Di Cesare PE, Rediske JJ, Abramson SB,. 1996. Effects of nitric oxide on chondrocyte migration, adhesion, and cytoskeletal assembly. Arthritis Rheum 39: 1905-1912.
11. Gao ZF, Yang ZZ, Ma KC, et al., 1985. The stimulative effect of injection epimedium on the growth of chick embryo femur in vitro. Chin J Int Trad Western Med 5 (3): 172-174.
12. Hsieh TP, Sheu SY, Sun JS, Chen MH, Liu MH,. 2010a. Icariin isolated from Epimedium pubescens regulates osteoblasts anabolism through BMP-2, SMAD4, and Cbfa1 expression. Phytomedicine 17: 414-423.
13. Hsieh TP, Sheu SY, Sun JS, Chen MH,. 2010b. Icariin inhibits osteoclast differentiation and bone resorption by suppression of MAPKs-NF-κB regulated HIF-1α and PGE2 synthesis. Phytomedicine doi: 10.1016/j.
14. Huang J, Yuan L, Wang X, Zhang TL, Wang K,. 2007. Icaritin and its glycosides enhance osteoblastic, but suppress osteoclastic, differentiation and activity in vitro. Life Sci 81: 832-840.
15. Kawamura K, Chu CR, Sobajima S,. 2005. Adenoviral-mediated transfer of TGF-β1 but not IGF-1 induces chondrogenic differentiation of human mesenchymal stem cells in pellet cultures. Exp Hematol 33: 865-872.
16. Kim Y, Sah R, Doong J, Grodzinsky AJ,. 1988. Flurometric assay of DNA in cartilage explants using Hoescht 33258. Anal Biochem 174: 168-176.
17. Ko CS, Huang JP, Huang CW, Chu IM,. 2009. Type II collagen-chondroitin sulfate-hyaluronan scaffold cross-linked by genipin for cartilage tissue engineering. J Biosci Bioeng 107 (2): 177-182.
18. Lin YC, Tan FJ, Marra KG, Jan SS, Liu DC,. 2009. Synthesis and characterization of collagen/hyaluronan/chitosan composite sponges for potential biomedical applications. Acta Biomater 5: 2591-2600.
19. Liu MH, Sun JS, Tsai SW, Sheu SY, Chen MH,. 2010. Icariin protects murine chondrocytes from lipopolysaccharide-induced inflammatory responses and extracellular matrix degradation. Nutr Res 30: 57-65.
20. Majumdar MK, Wang E, Morris EA,. 2001. BMP-2 and BMP-9 promotes chondrogenic differentiation of human multipotential mesenchymal cells and overcomes the inhibitory effect of IL-1. J Cell Physiol 189 (3): 275-284.
21. Meng FH, Li YB, Xiong ZL, Jiang ZM, Li FM,. 2005. Osteoblastic proliferative activity of Epimedium brevicornum Maxim. Phytomedicine 12: 189-193.
22. Plaas AHK, Wong-Palms S, Roughley PJ, Midura RJ, Hascall VC,. 1997. Chemical and immunological assay of the nonreducing terminal residues of chondroitin sulfate from human aggrecan. J Biol Chem 272: 20603-20610.
23. Qian G, Zhang X, Lu L, Wu X, Li S, Meng J,. 2006. Regulation of Cbfa1 expression by total flavonoids of Herba epimedii. Endocrine J 53 (1): 87-94.
24. Qureshi HY, Ahmad R, Sylvester J, Zafarullah M,. 2007. Requirement of phosphatidylinositol 3-kinase/Akt signaling pathway for regulation of tissue inhibitor of metallopro-teinases-3 gene expression by TGF-β in human chondrocytes. Cell Signal 19 (8): 1643-1651.
25. Roman-Blas JA, Stokes DG, Jimenez SA,. 2007. Modulation of TGF-beta signaling by proinflammatory cytokines in articular chondrocytes. Osteoarthr Cartilage 15 (12): 1367-1377.
26. Tchetina EV, Antoniou J, Tanzer M, Zukor DJ, Poole AR,. 2006. Transforming growth factor-beta2 suppresses collagen cleavage in cultured human osteoarthritic cartilage, reduces expression of genes associated with chondrocyte hypertrophy and degradation, and increases prostaglandin E(2) production. Am J Pathol 168 (1): 131-140.
27. Tew SR, Li Y, Pothacharoen P, Tweats LM, Hawkins RE, Hardingham TE,. 2005. Retroviral transduction with sox9 enhances re-expression of the chondrocyte phenotype in passaged osteoarthritic human articular chondrocytes. Osteoarthr Cartilage 13: 80-89.
28. Uebersax L, Merkle Hans P, Meinel L,. 2008. Insulin-like growth factor I releasing silk fibroin scaffolds induce chondrogenic differentiation of human mesenchymal stem cells. J Controlled Release 127: 12-21.
29. Varghese SH, Nathaniel S, Canver AC, Theprungsirikul P, Lin DW, Elisseeff J,. 2008. Chondroitin sulfate based niches for chondrogenic differentiation of mesenchymal stem cells. Matrix Biol 27: 12-21.
30. Wang B, Quan J, Guo S,. 2000. Effects of epimedium on the expression of interleukin-6 messenger ribonucleic acid in bone of ovariectomized rat. Chin J Obstet Gynecol (China) 35: 724-726.
31. Xie F, Wu C, Lai W, et al., 2005. The osteoprotective effect of Herba epimedii (HEP) extract in vivo and in vitro. Evid Based Compl Alt Med 2: 353-361.
32. Xu CQ, Liu BJ, Wu JF, et al., 2010. Icariin attenuates LPS-induced acute inflammatory responses: Involvement of PI3K/Akt and NF-κB signaling pathway. Eur J Pharmacol 642: 146-153.
33. Xue Y, Wang P, Qi Q, Ni H, Ma X, Guo S,. 2005. The experimental study of the effects of icariin on increasing smad4 mRNA level in MC3T3-E1 cell in vitro. Chin J Orthop (China) 25: 119-123.
34. Yeung HC,. 1985. Handbook of Chinese Herbs and Formulas. Institute of Chinese Medicine: Los Angeles.
35. Zhao J, Ohba S, Shinkai M, Chung UI, Nagamune T,. 2008. Icariin induces osteogenic differentiation in vitro in a BMP- and Runx2-dependent manner. Biochem Biophys Res Commun 369: 444-448.
36. Zhao J, Ohba S, Komiyama Y, Shinkai M, Chung U, Nagamune T,. 2010. Icariin: a potential osteoinductive compound for bone tissue engineering. Tissue Eng Part A 16: 233-243.