Expression and characterization of a functional single-chain variable fragment (scFv) protein recognizing MCF7 breast cancer cells in E. coli cytoplasm

Biochemical Genetics

Volumn 50, Issue 7-8, 2012, Pages 625-641

  Mahgoub, Ilham Omer ^a  

^a AL NEELAIN UNIVERSITY   (Sudan)

Author keywords

Bacterial cytoplasm expression; Breast cancer cells; Flow cytometry analysis; Immunofluorescence; Origami DE3; Protein solubility; Single chain fragment

Indexed keywords

DNA; EPITOPE; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; EPIDERMAL GROWTH FACTOR RECEPTOR;

ANTIBODY SPECIFICITY; ANTIGEN RECOGNITION; ARTICLE; BACTERIAL CELL; BINDING AFFINITY; BREAST CANCER; CANCER CELL CULTURE; CELL INTERACTION; CONTROLLED STUDY; CYTOPLASM; DNA SEQUENCE; ESCHERICHIA COLI; FLOW CYTOMETRY; IMMUNE RESPONSE GENE; IMMUNOASSAY; IMMUNOFLUORESCENCE; MOLECULAR CLONING; MOLECULAR RECOGNITION; NONHUMAN; PROTEIN BINDING; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN PURIFICATION; SINGLE CHAIN FRAGMENT VARIABLE GENE; WESTERN BLOTTING; BREAST TUMOR; CHEMISTRY; CYTOLOGY; GENE EXPRESSION; GENETICS; HUMAN; IMMUNOLOGY; ISOLATION AND PURIFICATION; PATHOLOGY; SOLUBILITY; TUMOR CELL LINE;

ANTIBODY SPECIFICITY; BREAST NEOPLASMS; CELL LINE, TUMOR; CLONING, MOLECULAR; CYTOPLASM; EPITOPES; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; RECEPTOR, EPIDERMAL GROWTH FACTOR; SEQUENCE ANALYSIS, DNA; SINGLE-CHAIN ANTIBODIES; SOLUBILITY;

BACTERIA (MICROORGANISMS);

EID: 84865978321     PISSN: 00062928     EISSN: 15734927     Source Type: Journal    
DOI: 10.1007/s10528-012-9506-4     Document Type: Article

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