A bacterial-two-hybrid selection system for one-step isolation of intracellularly functional Nanobodies

Archives of Biochemistry and Biophysics

Volumn 526, Issue 2, 2012, Pages 114-123

  Pellis, Mireille ^a,b   Pardon, Els ^a,b   Zolghadr, Kourosh ^c   Rothbauer, Ulrich ^c   Vincke, Cécile ^a,b   Kinne, Joerg ^d   Dierynck, Inge ^e   Hertogs, Kurt ^e   Leonhardt, Heinrich ^f   Messens, Joris ^a,b   Muyldermans, Serge ^a,b   Conrath, Katja ^a,b  

^a VRIJE UNIVERSITEIT BRUSSEL   (Belgium)

^b DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

^c CHROMOTEK GMBH   (Germany)

^d CENTRAL VETERINARY RESEARCH LABORATORY   (United Arab Emirates)

^e Virco BVBA   (Belgium)

^f UNIVERSITY OF MUNICH   (Germany)

Author keywords

Bacterial two hybrid; Intrabody; Nanobody

Indexed keywords

GREEN FLUORESCENT PROTEIN; INTEGRASE; NUCLEOSIDASE; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANIMAL CELL; ANTIBODY ISOLATION; ANTIGEN SPECIFICITY; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; DROMEDARY; MOLECULAR HYBRIDIZATION; MOLECULAR LIBRARY; MOLECULAR STABILITY; NONHUMAN; PHAGE DISPLAY; PRIORITY JOURNAL; TWO HYBRID SYSTEM;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODY AFFINITY; CAMELS; CELL LINE; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION; GREEN FLUORESCENT PROTEINS; HIV INTEGRASE; HIV-1; IMMUNOGLOBULIN FRAGMENTS; MOLECULAR SEQUENCE DATA; N-GLYCOSYL HYDROLASES; PEPTIDE LIBRARY; PROTEIN STABILITY; TRYPANOSOMA VIVAX;

BACTERIA (MICROORGANISMS); HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 84865690100     PISSN: 00039861     EISSN: 10960384     Source Type: Journal    
DOI: 10.1016/j.abb.2012.04.023     Document Type: Article

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