Molecular characterization of three 3-ketosteroid-Δ1- dehydrogenase isoenzymes of Rhodococcus ruber strain Chol-4

Journal of Steroid Biochemistry and Molecular Biology

Volumn 132, Issue 3-5, 2012, Pages 271-281

  Fernández De Las Heras, Laura ^a   Van Der Geize, Robert ^b   Drzyzga, Oliver ^a   Perera, Julián ^a   María Navarro Llorens, Juana ^a  

^a UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

^b UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

3 Ketosteroid 1 dehydrogenase; 4 Androstene 3,17 dione; Cholesterol; Rhodococcus ruber strain Chol 4

Indexed keywords

3 KETOSTEROID DELTA1 DEHYDROGENASE; ANDROSTENEDIONE; BACTERIAL ENZYME; BOLDIONE; CHOLESTEROL; ISOENZYME; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; CATABOLISM; COMAMONAS TESTOSTERONI; CONTROLLED STUDY; GENOME; MOLECULAR BIOLOGY; MUTANT; MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; RHODOCOCCUS; RHODOCOCCUS ERYTHROPOLIS; RHODOCOCCUS RUBER;

ANDROSTADIENES; ANDROSTENEDIONE; CHOLESTEROL; CULTURE MEDIA; GENE DELETION; GENETIC COMPLEMENTATION TEST; GENOME, BACTERIAL; ISOENZYMES; MOLECULAR SEQUENCE DATA; OXIDOREDUCTASES; RHODOCOCCUS;

BACTERIA (MICROORGANISMS); COMAMONAS TESTOSTERONI; RHODOCOCCUS ERYTHROPOLIS; RHODOCOCCUS RUBER;

EID: 84865569507     PISSN: 09600760     EISSN: 18791220     Source Type: Journal    
DOI: 10.1016/j.jsbmb.2012.06.005     Document Type: Article

References (49)

1. R. van der Geize, and L. Dijkhuizen Harnessing the catabolic diversity of rhodococci for environmental and biotechnological applications Current Opinion in Microbiology 7 3 2004 255 261
2. D. Vereecke, S. Burssens, C. Simon-Mateo, D. Inze, M. Van Montagu, K. Goethals, and M. Jaziri The Rhodococcus fascians-plant interaction: morphological traits and biotechnological applications Planta 210 2 2000 241 251
3. R. Plaggenborg, J. Overhage, A. Loos, J.A. Archer, P. Lessard, A.J. Sinskey, A. Steinbuchel, and H. Priefert Potential of Rhodococcus strains for biotechnological vanillin production from ferulic acid and eugenol Applied Microbiology and Biotechnology 72 4 2006 745 755
4. M. Kobayashi, and S. Shimizu Metalloenzyme nitrile hydratase: structure, regulation, and application to biotechnology Nature Biotechnology 16 8 1998 733 736
5. O. Bortolini, A. Medici, and S. Poli Biotransformations on steroid nucleus of bile acids Steroids 62 8-9 1997 564 577
6. K. Kieslich Microbial side-chain degradation of sterols Journal of Basic Microbiology 25 1985 461 474
7. S.B. Mahato, E. Mukherjee, and S. Banerjee Advances in microbial biotechnology of bile acids Biotechnology Advances 12 2 1994 357 391
8. K.C. Yam, and S. Okamoto Adventures in Rhodococcus - from steroids to explosives Canadian Journal of Microbiology 57 3 2011 155 168
9. S.T. Thomas, B.C. Vanderven, D.R. Sherman, D.G. Russell, and N.S. Sampson Pathway Profiling in Mycobacterium tuberculosis: elucidation of cholesterol-derived catabolite and enzymes that catalyze its metabolism Journal of Biological Chemistry 286 51 2011 43668 43678
10. R. van der Geize, K. Yam, T. Heuser, M.H. Wilbrink, H. Hara, M.C. Anderton, E. Sim, L. Dijkhuizen, J.E. Davies, W.W. Mohn, and L.D. Eltis A gene cluster encoding cholesterol catabolism in a soil actinomycete provides insight into Mycobacterium tuberculosis survival in macrophages Proceedings of the National Academy of Sciences of the United States of America 104 6 2007 1947 1952
11. J.E. Griffin, J.D. Gawronski, M.A. Dejesus, T.R. Ioerger, B.J. Akerley, and C.M. Sassetti High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism PLoS Pathogens 7 9 2011 e1002251
12. J.L. García, I. Uhía, and B. Galan Catabolism and biotechnological applications of cholesterol degrading bacteria Microbial Biotechnology 2012 http://dx.doi.org/10.1111/j.1751-7915.2012.00331.x
13. I. Uhía, B. Galán, S.L. Kendall, N.G. Stoker, and J.L. García Cholesterol metabolism in Mycobacterium smegmatis Environmental Microbiology Reports 4 2 2012 168 182
14. J.K. Capyk, I. Casabon, R. Gruninger, N.C. Strynadka, and L.D. Eltis Activity of 3-ketosteroid 9α-hydroxylase (KshAB) indicates cholesterol side chain and ring degradation occur simultaneously in Mycobacterium tuberculosis Journal of Biological Chemistry 286 47 2011 40717 40724
15. C. Dresen, L.Y. Lin, I. D'Angelo, E.I. Tocheva, N. Strynadka, and L.D. Eltis A flavin-dependent monooxygenase from Mycobacterium tuberculosis involved in cholesterol catabolism Journal of Biological Chemistry 285 29 2010 22264 22275
16. K.Z. Rosłoniec, M.H. Wilbrink, J.K. Capyk, W.W. Mohn, M. Ostendorf, R. van der Geize, L. Dijkhuizen, and L.D. Eltis Cytochrome P450 125 (CYP125) catalyses C26-hydroxylation to initiate sterol side-chain degradation in Rhodococcus jostii RHA1 Molecular Microbiology 74 5 2009 1031 1043
17. R. van der Geize, G.I. Hessels, R. van Gerwen, P. van der Meijden, and L. Dijkhuizen Molecular and functional characterization of kshA and kshB, encoding two components of 3-ketosteroid 9α-hydroxylase, a class IA monooxygenase, in Rhodococcus erythropolis strain SQ1 Molecular Microbiology 45 4 2002 1007 1018
18. J.K. Capyk, I. D'Angelo, N.C. Strynadka, and L.D. Eltis Characterization of 3-ketosteroid 9α-hydroxylase, a rieske oxygenase in the cholesterol degradation pathway of Mycobacterium tuberculosis The Journal of Biological Chemistry 284 15 2009 9937 9946
19. M. Horinouchi, T. Hayashi, and T. Kudo Steroid degradation in Comamonas testosteroni Journal of Steroid Biochemistry and Molecular Biology 129 1-2 2012 4 14
20. B. Philipp Bacterial degradation of bile salts Applied Microbiology and Biotechnology 89 4 2011 903 915
21. 1-dehydrogenase from Nocardia corallina Biochimica et Biophysica Acta 1040 2 1990 281 286
22. 1-dehydrogenase isoenzyme Microbiology 148 Pt 10 2002 3285 3292
23. 1-dehydrogenase isoenzyme of Rhodococcus erythropolis strain SQ1 Applied and Environmental Microbiology 66 5 2000 2029 2036
24. M. Kisiela, A. Skarka, B. Ebert, and E. Maser Hydroxysteroid dehydrogenases (HSDs) in bacteria - a bioinformatic perspective Journal of Steroid Biochemistry and Molecular Biology 129 1-2 2012 31 46
25. 1-dehydrogenase from Rhodococcus erythropolis SQ1 and its orthologue in Mycobacterium tuberculosis H37Rv are highly specific enzymes that function in cholesterol catabolism Biochemical Journal 410 2 2008 339 346
26. L. Fernández de las Heras, E. García Fernández, J. María Navarro Llorens, J. Perera, and O. Drzyzga Morphological, physiological, and molecular characterization of a newly isolated steroid-degrading actinomycete, identified as Rhodococcus ruber strain Chol-4 Current Microbiology 59 5 2009 548 553
27. J. Sambrook, E.F. Fritsch, and T. Maniatis Molecular Cloning: A Laboratory Manual 2nd edition 1989 Cold Spring Harbor Laboratory Press NY
28. L. Fernández de Las Heras, V. Mascaraque, E. García Fernandez, J.M. Navarro-Llorens, J. Perera, and O. Drzyzga ChoG is the main inducible extracellular cholesterol oxidase of Rhodococcus sp. strain CECT3014 Microbiological Research 166 5 2011 403 418
29. K. Chiba, Y. Hoshino, K. Ishino, T. Kogure, Y. Mikami, Y. Uehara, and J. Ishikawa Construction of a pair of practical Nocardia-Escherichia coli shuttle vectors Japanese Journal of Infectious Diseases 60 1 2007 45 47
30. J. Hosek, P. Svastova, M. Moravkova, I. Pavlik, and M. Bartos Methods of mycobacterial DNA isolation from different biological material: a review Veterinary Research 524 2006 180 192
31. T.A. Hall BioEdit a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT Nucleic Acids Symposium Series 41 1999 95 98
32. 1-dehydrogenase, in Rhodococcus erythropolis SQ1 using sacB as counter-selectable marker FEMS Microbiology Letters 205 2 2001 197 202
33. A. Schafer, A. Tauch, W. Jager, J. Kalinowski, G. Thierbach, and A. Puhler Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum Gene 145 1 1994 69 73
34. M.P. McLeod, R.L. Warren, W.W. Hsiao, N. Araki, M. Myhre, C. Fernandes, D. Miyazawa, W. Wong, A.L. Lillquist, and D. Wang The complete genome of Rhodococcus sp. RHA1 provides insights into a catabolic powerhouse Proceedings of the National Academy of Sciences of the United States of America 103 42 2006 15582 15587
35. 5-isomerase and a hypothetical regulatory protein Molecular Microbiology 15 5 1995 895 905
36. 1-dehydrogenase of Rhodococcus rhodochrous: sequencing of the genomic DNA and hyperexpression, purification, and characterization of the recombinant enzyme Journal of Biochemistry 124 5 1998 1026 1032
37. M.G. Wovcha, K.E. Brooks, and L.A. Kominek Evidence for two steroid 1,2-dehydrogenase activities in Mycobacterium fortuitum Biochimica et Biophysica Acta 574 3 1979 471 479
38. J.M. Mathieu, W.W. Mohn, L.D. Eltis, J.C. LeBlanc, G.R. Stewart, C. Dresen, K. Okamoto, and P.J. Alvarez 7-Ketocholesterol catabolism by Rhodococcus jostii RHA1 Applied and Environment Microbiology 76 1 2010 352 355
39. G. Burns, T. Brown, K. Hatter, and J.R. Sokatch Sequence analysis of the lpdV gene for lipoamide dehydrogenase of branched-chain-oxoacid dehydrogenase of Pseudomonas putida European Journal of Biochemistry 179 1989 61 69
40. 4(5α)-dehydrogenase: gene and protein characterization Journal of Bacteriology 178 11 1996 3322 3330
41. 1-dehydrogenase from Rhodococcus rhodochrous Journal of Biochemistry 126 4 1999 662 667
42. S.L. Kendall, P. Burgess, R. Balhana, M. Withers, A. Ten Bokum, J.S. Lott, C. Gao, I. Uhía-Castro, and N.G. Stoker Cholesterol utilization in mycobacteria is controlled by two TetR-type transcriptional regulators: kstR and kstR2 Microbiology 156 Pt 5 2010 1362 1371
43. S.L. Kendall, M. Withers, C.N. Soffair, N.J. Moreland, S. Gurcha, B. Sidders, R. Frita, A. Ten Bokum, G.S. Besra, J.S. Lott, and N.G. Stoker A highly conserved transcriptional repressor controls a large regulon involved in lipid degradation in Mycobacterium smegmatis and Mycobacterium tuberculosis Molecular Microbiology 65 3 2007 684 699
44. J. Kreit, and N.S. Sampson Cholesterol oxidase: physiological functions FEBS Journal 276 23 2009 6844 6856
45. L. Pollegioni, L. Piubelli, and G. Molla Cholesterol oxidase: biotechnological applications FEBS Journal 276 2009 6857 6870
46. A. Vrielink, and S. Ghisla Cholesterol oxidase: biochemistry and structural features FEBS Journal 276 23 2009 6826 6843
47. A. Brzostek, T. Sliwinski, A. Rumijowska-Galewicz, M. Korycka-Machala, and J. Dziadek Identification and targeted disruption of the gene encoding the main 3-ketosteroid dehydrogenase in Mycobacterium smegmatis Microbiology 151 Pt 7 2005 2393 2402
48. M. Petrusma, G. Hessels, L. Dijkhuizen, and R. van der Geize Multiplicity of 3-ketosteroid-9α-hydroxylase enzymes in Rhodococcus rhodochrous DSM43269 for specific degradation of different classes of steroids Journal of Bacteriology 193 15 2011 3931 3940
49. R. van der Geize, G.I. Hessels, M. Nienhuis-Kuiper, and L. Dijkhuizen Characterization of a second Rhodococcus erythropolis SQ1 3-ketosteroid 9α-hydroxylase activity comprising a terminal oxygenase homologue, KshA2, active with oxygenase-reductase component KshB Applied and Environmental Microbiology 74 23 2008 7197 7203