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1
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0028002904
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Biotransformations catalyzed by the genus Rhodococcus
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Warhurst A.M., Fewson C.A. Biotransformations catalyzed by the genus Rhodococcus. Crit Rev Biotech. 14:1994;29-73
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Warhurst, A.M.1
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0037457699
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Microbial conversion of steroid compounds: Recent developments
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Fernandes P., Cruz A., Angelova B., Pinheiro H.M., Cabral J.M.S. Microbial conversion of steroid compounds: recent developments. Enzyme Microbial Tech. 32:2003;688-705
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Enzyme Microbial Tech
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Fernandes, P.1
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Angelova, B.3
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4
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0026715272
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The biology and genetics of the genus Rhodococcus
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Finnerty W.R. The biology and genetics of the genus Rhodococcus. Annu Rev Microbiol. 46:1992;193-218
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Annu Rev Microbiol
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Finnerty, W.R.1
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6
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0035350114
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Characterization of the 450-kb linear plasmid in a polychlorinated biphenyl degrader, Rhodococcus sp. strain RHA1
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Shimizu S., Kobayashi H., Masai E., Fukuda M. Characterization of the 450-kb linear plasmid in a polychlorinated biphenyl degrader, Rhodococcus sp. strain RHA1. Appl Environ Microbiol. 67:2001;2021-2028
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Appl Environ Microbiol
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Shimizu, S.1
Kobayashi, H.2
Masai, E.3
Fukuda, M.4
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7
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0042389561
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Complete nucleotide sequence and genetic organization of the 210-kilobase linear plasmid of Rhodococcus erythropolis BD2
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This paper reports the complete nucleotide sequence of a rhodococcal linear plasmid revealing a large number of open reading frames (ORFs) involved in transposition and catabolism. The authors suggest that certain catabolic pathways may have been distributed among Gram-positive soil bacteria via linear plasmid-mediated horizontal gene transfer. The presence of a large number of transposon-related ORFs on this linear plasmid suggests that such plasmids can undergo dynamic rearrangements at a high frequency.
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Stecker C., Johann A., Herzberg C., Averhoff B., Gottschalk G. Complete nucleotide sequence and genetic organization of the 210-kilobase linear plasmid of Rhodococcus erythropolis BD2. J Bacteriol. 185:2003;5269-5274 This paper reports the complete nucleotide sequence of a rhodococcal linear plasmid revealing a large number of open reading frames (ORFs) involved in transposition and catabolism. The authors suggest that certain catabolic pathways may have been distributed among Gram-positive soil bacteria via linear plasmid-mediated horizontal gene transfer. The presence of a large number of transposon-related ORFs on this linear plasmid suggests that such plasmids can undergo dynamic rearrangements at a high frequency.
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(2003)
J Bacteriol
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, pp. 5269-5274
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Stecker, C.1
Johann, A.2
Herzberg, C.3
Averhoff, B.4
Gottschalk, G.5
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8
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0030740918
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Conservation of plasmid-encoded dibenzothiophene desulphurization genes in several rhodococci
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Denis-Larose C., Labbe D., Bergeron H., Jones A.M., Greer C.W., al-Hawari J., Grossman M.J., Sankey B.M., Lau P.C. Conservation of plasmid-encoded dibenzothiophene desulphurization genes in several rhodococci. Appl Environ Microbiol. 63:1997;2915-2919
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Appl Environ Microbiol
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Denis-Larose, C.1
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Bergeron, H.3
Jones, A.M.4
Greer, C.W.5
Al-Hawari, J.6
Grossman, M.J.7
Sankey, B.M.8
Lau, P.C.9
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9
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0036304123
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Monocyclic aromatic hydrocarbon degradation by Rhodococcus sp. strain DK17
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Kim D., Kim Y.S., Kim S.K., Kim S.W., Zylstra G.J., Kim Y.M., Kim E. Monocyclic aromatic hydrocarbon degradation by Rhodococcus sp. strain DK17. Appl Environ Microbiol. 68:2002;3270-3278
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Appl Environ Microbiol
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, pp. 3270-3278
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Kim, D.1
Kim, Y.S.2
Kim, S.K.3
Kim, S.W.4
Zylstra, G.J.5
Kim, Y.M.6
Kim, E.7
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10
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0242669334
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Gene cloning and characterization of multiple alkane hydroxylase systems in Rhodococcus strains Q15 and NRRL B-16531
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This study presents a strong example of the apparent redundancy in catabolic genes present in the genus Rhodococcus. At least four alkane hydroxylases alkB gene homologs were found in two rhodococcal strains. Characterisation of these alkane hydroxylases suggests that the presence of several homologs broadens the catabolic capabilities of the strain. Several gene homologs were identified in eight other Rhodococcus strains, indicating that multiple alkane hydroxylases may be a common feature of Rhodococcus strains.
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Whyte L.G., Smits T.H., Labbe D., Witholt B., Greer C.W., van Beilen J.B. Gene cloning and characterization of multiple alkane hydroxylase systems in Rhodococcus strains Q15 and NRRL B-16531. Appl Environ Microbiol. 68:2002;5933-5942 This study presents a strong example of the apparent redundancy in catabolic genes present in the genus Rhodococcus. At least four alkane hydroxylases alkB gene homologs were found in two rhodococcal strains. Characterisation of these alkane hydroxylases suggests that the presence of several homologs broadens the catabolic capabilities of the strain. Several gene homologs were identified in eight other Rhodococcus strains, indicating that multiple alkane hydroxylases may be a common feature of Rhodococcus strains.
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(2002)
Appl Environ Microbiol
, vol.68
, pp. 5933-5942
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Whyte, L.G.1
Smits, T.H.2
Labbe, D.3
Witholt, B.4
Greer, C.W.5
Van Beilen, J.B.6
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11
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18744416524
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Alkane hydroxylase homologues in Gram-positive strains
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This study shows the usefulness of PCR with degenerate primers to identify gene homologs, and thus the genetic basis for catabolic diversity in Rhodococcus. In this case three to five, quite divergent, alkane hydroxylase alkB gene homologs were amplified from Rhodococus isolates.
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van Beilen J.B., Smits T.H., Whyte L.G., Schorcht S., Rothlisberger M., Plaggemeier T., Engesser K.H., Witholt B. Alkane hydroxylase homologues in Gram-positive strains. Environ Microbiol. 4:2002;676-682 This study shows the usefulness of PCR with degenerate primers to identify gene homologs, and thus the genetic basis for catabolic diversity in Rhodococcus. In this case three to five, quite divergent, alkane hydroxylase alkB gene homologs were amplified from Rhodococus isolates.
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(2002)
Environ Microbiol
, vol.4
, pp. 676-682
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Van Beilen, J.B.1
Smits, T.H.2
Whyte, L.G.3
Schorcht, S.4
Rothlisberger, M.5
Plaggemeier, T.6
Engesser, K.H.7
Witholt, B.8
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12
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0037312775
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Characterization of extradiol dioxygenases from a polychlorinated biphenyl-degrading strain that possess higher specificities for chlorinated metabolites
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Vaillancourt F.H., Haro M.A., Drouin N.M., Karim Z., Maaroufi H., Eltis L.D. Characterization of extradiol dioxygenases from a polychlorinated biphenyl-degrading strain that possess higher specificities for chlorinated metabolites. J Bacteriol. 185:2003;1253-1260
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(2003)
J Bacteriol
, vol.185
, pp. 1253-1260
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Vaillancourt, F.H.1
Haro, M.A.2
Drouin, N.M.3
Karim, Z.4
Maaroufi, H.5
Eltis, L.D.6
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13
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0037408540
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Substrate specificity and expression of three 2,3-dihydroxybiphenyl 1,2-dioxygenases from Rhodococcus globerulus strain P6
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McKay D.B., Prucha M., Reineke W., Timmis K.N., Pieper D.H. Substrate specificity and expression of three 2, 3-dihydroxybiphenyl 1, 2-dioxygenases from Rhodococcus globerulus strain P6. J Bacteriol. 185:2003;2944-2951
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(2003)
J Bacteriol
, vol.185
, pp. 2944-2951
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McKay, D.B.1
Prucha, M.2
Reineke, W.3
Timmis, K.N.4
Pieper, D.H.5
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14
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0036654304
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Isolation and characterization of dibenzofuran-degrading actinomycetes: Analysis of multiple extradiol dioxygenase genes in dibenzofuran-degrading Rhodococcus species
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Iida T., Mukouzaka Y., Nakamura K., Yamaguchi I., Kudo T. Isolation and characterization of dibenzofuran-degrading actinomycetes: analysis of multiple extradiol dioxygenase genes in dibenzofuran-degrading Rhodococcus species. Biosci Biotechnol Biochem. 66:2002;1462-1472
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(2002)
Biosci Biotechnol Biochem
, vol.66
, pp. 1462-1472
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Iida, T.1
Mukouzaka, Y.2
Nakamura, K.3
Yamaguchi, I.4
Kudo, T.5
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15
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0031779621
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Two nearly identical aromatic compound hydrolase genes in a strong polychlorinated biphenyl degrader, Rhodococcus sp. strain RHA1
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Yamada A., Kishi H., Sugiyama K., Hatta T., Nakamura K., Masai E., Fukuda M. Two nearly identical aromatic compound hydrolase genes in a strong polychlorinated biphenyl degrader, Rhodococcus sp. strain RHA1. Appl Environ Microbiol. 64:1998;2006-2012
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, pp. 2006-2012
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Yamada, A.1
Kishi, H.2
Sugiyama, K.3
Hatta, T.4
Nakamura, K.5
Masai, E.6
Fukuda, M.7
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16
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0029969173
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Growth inhibition of Rhodococcus sp. strain RHA1 in the course of PCB transformation
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Seto M., Okita N., Sugiyama k., Masai E., Fukuda M. Growth inhibition of Rhodococcus sp. strain RHA1 in the course of PCB transformation. Biotechnol Lett. 18:1996;1193-1198
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, vol.18
, pp. 1193-1198
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Seto, M.1
Okita, N.2
Sugiyama, K.3
Masai, E.4
Fukuda, M.5
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17
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0037224809
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2-Hydroxypenta-2,4-dienoate metabolic pathway genes in a strong polychlorinated biphenyl degrader, Rhodococcus sp. strain RHA1
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Sakai M., Miyauchi K., Kato N., Masai E., Fukuda M. 2-Hydroxypenta-2, 4-dienoate metabolic pathway genes in a strong polychlorinated biphenyl degrader, Rhodococcus sp. strain RHA1. Appl Environ Microbiol. 69:2003;427-433
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(2003)
Appl Environ Microbiol
, vol.69
, pp. 427-433
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Sakai, M.1
Miyauchi, K.2
Kato, N.3
Masai, E.4
Fukuda, M.5
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18
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1342314487
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PCB/biphenyl degradation gene cluster in Rhodococcus rhodochrous K37, is different from the well-known bph gene cluster in Rhodococcus sp. P6, RHA1, and TA421
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Taguchi K., Motoyama M., Kudo T. PCB/biphenyl degradation gene cluster in Rhodococcus rhodochrous K37, is different from the well-known bph gene cluster in Rhodococcus sp. P6, RHA1, and TA421. RIKEN Rev. 42:2001;23-26
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(2001)
RIKEN Rev
, vol.42
, pp. 23-26
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Taguchi, K.1
Motoyama, M.2
Kudo, T.3
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19
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0036777640
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A new modified ortho cleavage pathway of 3-chlorocatechol degradation by Rhodococcus opacus 1CP: Genetic and biochemical evidence
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Moiseeva O.V., Solyanikova I.P., Kaschabek S.R., Groning J., Thiel M., Golovleva L.A., Schlomann M. A new modified ortho cleavage pathway of 3-chlorocatechol degradation by Rhodococcus opacus 1CP: genetic and biochemical evidence. J Bacteriol. 184:2002;5282-5292
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(2002)
J Bacteriol
, vol.184
, pp. 5282-5292
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Moiseeva, O.V.1
Solyanikova, I.P.2
Kaschabek, S.R.3
Groning, J.4
Thiel, M.5
Golovleva, L.A.6
Schlomann, M.7
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20
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0034660164
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Desulphurization of alkylated forms of both dibenzothiophene and benzothiophene by a single bacterial strain
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Kobayashi M., Onaka T., Ishii Y., Konishi J., Takaki M., Okada H., Ohta Y., Koizumi K., Suzuki M. Desulphurization of alkylated forms of both dibenzothiophene and benzothiophene by a single bacterial strain. FEMS Microbiol Lett. 187:2000;123-126
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(2000)
FEMS Microbiol Lett
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, pp. 123-126
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Kobayashi, M.1
Onaka, T.2
Ishii, Y.3
Konishi, J.4
Takaki, M.5
Okada, H.6
Ohta, Y.7
Koizumi, K.8
Suzuki, M.9
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21
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0036315398
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Biodesulphurization of benzothiophene and dibenzothiophene by a newly isolated Rhodococcus strain
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Tanaka Y., Matsui T., Konishi J., Maruhashi K., Kurane R. Biodesulphurization of benzothiophene and dibenzothiophene by a newly isolated Rhodococcus strain. Appl Microbiol Biotechnol. 59:2002;325-328
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(2002)
Appl Microbiol Biotechnol
, vol.59
, pp. 325-328
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Tanaka, Y.1
Matsui, T.2
Konishi, J.3
Maruhashi, K.4
Kurane, R.5
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22
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0036330524
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Biodesulphurization of naphthothiophene and benzothiophene through selective cleavage of carbon-sulfur bonds by Rhodococcus sp. strain WU-K2R
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Kirimura K., Furuya T., Sato R., Ishii Y., Kino K., Usami S. Biodesulphurization of naphthothiophene and benzothiophene through selective cleavage of carbon-sulfur bonds by Rhodococcus sp. strain WU-K2R. Appl Environ Microbiol. 68:2002;3867-3872
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(2002)
Appl Environ Microbiol
, vol.68
, pp. 3867-3872
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Kirimura, K.1
Furuya, T.2
Sato, R.3
Ishii, Y.4
Kino, K.5
Usami, S.6
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23
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0027996697
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Characterization of the desulphurization genes from Rhodococcus sp. strain IGTS8
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Denome S.A., Oldfield C., Nash L.J., Young K.D. Characterization of the desulphurization genes from Rhodococcus sp. strain IGTS8. J Bacteriol. 176:1994;6707-6716
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J Bacteriol
, vol.176
, pp. 6707-6716
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Denome, S.A.1
Oldfield, C.2
Nash, L.J.3
Young, K.D.4
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24
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0028836453
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Sequence and molecular characterization of a DNA region encoding the dibenzothiophene desulphurization operon of Rhodococcus sp. strain IGTS8
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Piddington C.S., Kovacevich B.R., Rambosek J. Sequence and molecular characterization of a DNA region encoding the dibenzothiophene desulphurization operon of Rhodococcus sp. strain IGTS8. Appl Environ Microbiol. 61:1995;468-475
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Appl Environ Microbiol
, vol.61
, pp. 468-475
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Piddington, C.S.1
Kovacevich, B.R.2
Rambosek, J.3
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25
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0029946460
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Genetic analysis of the dsz promoter and associated regulatory regions of Rhodococcus erythropolis IGTS8
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Li M.Z., Squires C.H., Monticello D.J., Childs J.D. Genetic analysis of the dsz promoter and associated regulatory regions of Rhodococcus erythropolis IGTS8. J Bacteriol. 178:1996;6409-6418
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J Bacteriol
, vol.178
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Li, M.Z.1
Squires, C.H.2
Monticello, D.J.3
Childs, J.D.4
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26
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0035258448
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Improvement of desulphurization activity in Rhodococcus erythropolis KA2-5-1 by genetic engineering
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Hirasawa K., Ishii Y., Kobayashi M., Koizumi K., Maruhashi K. Improvement of desulphurization activity in Rhodococcus erythropolis KA2-5-1 by genetic engineering. Biosci Biotechnol Biochem. 65:2001;239-246
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(2001)
Biosci Biotechnol Biochem
, vol.65
, pp. 239-246
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Hirasawa, K.1
Ishii, Y.2
Kobayashi, M.3
Koizumi, K.4
Maruhashi, K.5
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27
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0036780595
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Recombinant Rhodococcus sp. strain T09 can desulphurize DBT in the presence of inorganic sulfate
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This paper describes the expression of the dsz genes even in the presence of sulphate, methionine or cysteine, when under control of the Rhodococcus putative rrn promoter region. The two-phase (growth followed by induction) cultivation commonly applied in desulphurization of organosulphur compounds would be reduced to a single simple bioconversion step using the recombinant strain exhibiting constitutive dsz gene expression.
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Matsui T., Noda K., Tanaka Y., Maruhashi K., Kurane R. Recombinant Rhodococcus sp. strain T09 can desulphurize DBT in the presence of inorganic sulfate. Curr Microbiol. 45:2002;240-244 This paper describes the expression of the dsz genes even in the presence of sulphate, methionine or cysteine, when under control of the Rhodococcus putative rrn promoter region. The two-phase (growth followed by induction) cultivation commonly applied in desulphurization of organosulphur compounds would be reduced to a single simple bioconversion step using the recombinant strain exhibiting constitutive dsz gene expression.
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(2002)
Curr Microbiol
, vol.45
, pp. 240-244
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Matsui, T.1
Noda, K.2
Tanaka, Y.3
Maruhashi, K.4
Kurane, R.5
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28
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0042355547
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Enhanced desulphurization in a transposon-mutant strain of Rhodococcus erythropolis
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This paper reports the construction of a mutant in which the dszABCD genes for desulphurization were expressed using a rhodococcal promoter (kapI) that is not affected by the presence of sulphate. An elegant genetic tool, making use of a transposome complex, was adapted to stably integrate the expression cassette into the Rhodococcus genome.
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Watanabe K., Noda K., Maruhashi K. Enhanced desulphurization in a transposon-mutant strain of Rhodococcus erythropolis. Biotechnol Lett. 25:2003;1299-1304 This paper reports the construction of a mutant in which the dszABCD genes for desulphurization were expressed using a rhodococcal promoter (kapI) that is not affected by the presence of sulphate. An elegant genetic tool, making use of a transposome complex, was adapted to stably integrate the expression cassette into the Rhodococcus genome.
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(2003)
Biotechnol Lett
, vol.25
, pp. 1299-1304
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Watanabe, K.1
Noda, K.2
Maruhashi, K.3
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29
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0036854516
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Cloning of a rhodococcal promoter using a transposon for dibenzothiophene biodesulphurization
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A potentially widely applicable promoter-probe transposon with red-shifted gfp as a reporter gene was constructed to screen for rhodococcal promoters. The method was used to isolate a promoter that is not affected by the presence of sulfate.
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Noda K., Watanabe K., Maruhashi K. Cloning of a rhodococcal promoter using a transposon for dibenzothiophene biodesulphurization. Biotechnol Lett. 24:2002;1875-1882 A potentially widely applicable promoter-probe transposon with red-shifted gfp as a reporter gene was constructed to screen for rhodococcal promoters. The method was used to isolate a promoter that is not affected by the presence of sulfate.
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(2002)
Biotechnol Lett
, vol.24
, pp. 1875-1882
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Noda, K.1
Watanabe, K.2
Maruhashi, K.3
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30
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0036402921
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The cbs mutant strain of Rhodococcus erythropolis KA2-5-1 expresses high levels of Dsz enzymes in the presence of sulfate
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This paper describes the successful use of the transposome complex technique as a genetic tool for transposon mutagenesis in Rhodococcus. The study describes the isolation of cystathionine β-synthase (cbs) mutants of Rhodococcus erythropolis that are able to express the dsz genes, encoding desulphurization, even in the presence of high levels of sulfate.
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Tanaka Y., Yoshikawa O., Maruhashi K., Kurane R. The cbs mutant strain of Rhodococcus erythropolis KA2-5-1 expresses high levels of Dsz enzymes in the presence of sulfate. Arch Microbiol. 178:2002;351-357 This paper describes the successful use of the transposome complex technique as a genetic tool for transposon mutagenesis in Rhodococcus. The study describes the isolation of cystathionine β-synthase (cbs) mutants of Rhodococcus erythropolis that are able to express the dsz genes, encoding desulphurization, even in the presence of high levels of sulfate.
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(2002)
Arch Microbiol
, vol.178
, pp. 351-357
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Tanaka, Y.1
Yoshikawa, O.2
Maruhashi, K.3
Kurane, R.4
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31
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0035910121
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Random insertion mutagenesis of the intracellular pathogen Rhodococcus equi using transposomes
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Mangan M.W., Meijer W.G. Random insertion mutagenesis of the intracellular pathogen Rhodococcus equi using transposomes. FEMS Microbiol Lett. 205:2001;243-246
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(2001)
FEMS Microbiol Lett
, vol.205
, pp. 243-246
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Mangan, M.W.1
Meijer, W.G.2
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33
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0036038941
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Molecular and functional characterization of kshA and kshB, encoding two components of 3-ketosteroid 9α-hydroxylase, a class IA monooxygenase, in Rhodococcus erythropolis SQ1
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This paper is a good example of the use of the unmarked gene deletion technique for Rhodococcus cell engineering. It reports the construction of single as well as triple gene deletion mutants of R. erythropolis, introducing a metabolic block at the level of steroid ring opening. The triple gene deletion mutant in particular would have been difficult to construct without the use of unmarked gene deletion technology.
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Van der Geize R., Hessels G.I., Van Gerwen R., Van der Meijden P., Dijkhuizen L. Molecular and functional characterization of kshA and kshB, encoding two components of 3-ketosteroid 9α-hydroxylase, a class IA monooxygenase, in Rhodococcus erythropolis SQ1. Mol Microbiol. 45:2002;1007-1018 This paper is a good example of the use of the unmarked gene deletion technique for Rhodococcus cell engineering. It reports the construction of single as well as triple gene deletion mutants of R. erythropolis, introducing a metabolic block at the level of steroid ring opening. The triple gene deletion mutant in particular would have been difficult to construct without the use of unmarked gene deletion technology.
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(2002)
Mol Microbiol
, vol.45
, pp. 1007-1018
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Van Der Geize, R.1
Hessels, G.I.2
Van Gerwen, R.3
Van Der Meijden, P.4
Dijkhuizen, L.5
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35
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0032461682
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Molecular characterisation of a Rhodococcus oph operon
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Powell J.A.C., Archer J.A.C. Molecular characterisation of a Rhodococcus oph operon. Antonie van Leeuwenhoek. 74:1998;175-188
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(1998)
Antonie Van Leeuwenhoek
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, pp. 175-188
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Powell, J.A.C.1
Archer, J.A.C.2
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37
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0036127897
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Npd gene functions of Rhodococcus (opacus) erythropolis HL PM-1 in the initial steps of 2,4,6-trinitrophenol degradation
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Heiss G., Hofmann K.W., Trachtmann N., Walters D.M., Rouviere P., Knackmuss H.J. npd gene functions of Rhodococcus (opacus) erythropolis HL PM-1 in the initial steps of 2, 4, 6-trinitrophenol degradation. Microbiology. 148:2002;799-806
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(2002)
Microbiology
, vol.148
, pp. 799-806
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Heiss, G.1
Hofmann, K.W.2
Trachtmann, N.3
Walters, D.M.4
Rouviere, P.5
Knackmuss, H.J.6
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38
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0347622757
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NpdR, a repressor involved in 2,4,6-trinitrophenol degradation in Rhodococcus opacus HL PM-1
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Paper reporting the inactivation of a negative transcriptional regulator by the same unmarked gene deletion method, resulting in constitutive expression of genes involved in trinitrophenol degradation.
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Nga D.P., Altenbuchner J., Heiss G.S. NpdR, a repressor involved in 2, 4, 6-trinitrophenol degradation in Rhodococcus opacus HL PM-1. J Bacteriol. 186:2004;98-103 Paper reporting the inactivation of a negative transcriptional regulator by the same unmarked gene deletion method, resulting in constitutive expression of genes involved in trinitrophenol degradation.
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(2004)
J Bacteriol
, vol.186
, pp. 98-103
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Nga, D.P.1
Altenbuchner, J.2
Heiss, G.S.3
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