Comamonas testosteroni 3-ketosteroid-Δ4(5α)-dehydrogenase: Gene and protein characterization

Journal of Bacteriology

Volumn 178, Issue 11, 1996, Pages 3322-3330

  Florin, Claire ^a   Köhler, Thilo ^b   Grandguillot, Michel ^a   Plesiat, Patrick ^a  

^a Lab Physiologie et Pharmacologie   (France)

^b UNIVERSITY OF GENEVA MEDICAL SCHOOL   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

3 OXOSTEROID DELTA1 DEHYDROGENASE; BACTERIAL ENZYME; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BINDING SITE; COMAMONAS TESTOSTERONI; ENZYME DEGRADATION; ENZYME ISOLATION; GENE ISOLATION; MOLECULAR CLONING; PRIORITY JOURNAL; TRANSCRIPTION REGULATION;

EID: 0029895848     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.11.3322-3330.1996     Document Type: Article

References (48)

1. Abalain, J. H., S. Distefano, Y. Amet, E. Quemener, M. L. Abalaincolloc, and H. H. Floch. 1993. Cloning, DNA sequencing and expression of (3-17)beta-hydroxysteroid dehydrogenase from Pseudomonas testosterone. J. Steroid Biochem. Mol. Biol. 44:133-139.
2. Abul-Hajj, Y. J. 1972. Stereochemistry of C-1,2 dehydrogenation of 5β-pregnane-3,11,20-trione by Septomyxa affinis. J. Biol. Chem. 247:686-691.
3. Altschul, S. F., W. Gish, W. Miller, E. W. Myers, and D. J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
4. Benen, J. A., W. J. Van Berkel, W. M. Van Dongen, F. Muller, and A. De Kok. 1989. Molecular cloning and sequence determination of the lpd gene encoding lipoamide dehydrogenase from Pseudomonas fluorescens. J. Gen. Microbiol. 135:1787-1797.
5. Brown, N. L., S. J. Ford, R. D. Pridmore, and D. C. Fritzinger. 1983. Nucleotide sequence of the gene from the Pseudomonas transposon Tn501 encoding mercuric reductase. Biochemistry 22:4089-4095.
6. Burns, G., T. Brown, K. Hatter, and J. R. Sokatch. 1989. Sequence analysis of the lpdV gene for lipoamide dehydrogenase of branched-chain-oxoacid dehydrogenase of Pseudomonas putida. Eur. J. Biochem. 179:61-69.
7. Charney, W., and H. L. Herzog. 1967. Microbial transformations of steroids: a handbook. Academic Press, Inc., New York.
8. Choi, K. Y., and W. F. Benisek. 1988. Nucleotide sequence of the gene for the delta 5-3-ketosteroid isomerase of Pseudomonas testosteroni. Gene 69: 121-129.
9. Coulter, A. W., and P. Talalay. 1968. Studies on the microbiological degradation of steroid ring A. J. Biol. Chem. 243:3238-3247.
10. 4-5β-dehydrogenase. J. Biol. Chem. 241:906-915.
11. Dodson, R. M., and R. D. Muir. 1961. Microbiological transformations. VI. The microbiological aromatization of steroids. J. Am. Chem. Soc. 83:4627-4631.
12. Dower, W. J., J. F. Miller, and C. W. Ragsdale. 1988. High efficiency transformation of E. coli by high voltage electroporation. Nucleic Acids Res. 16:6127-6145.
13. Frantz, B., and A. M. Chakrabarty. 1986. Degradative plasmids in Pseudomonas, p. 295-323. In J. R. Sokatch (ed.), The bacteria: a treatise on structure and function. Academic Press, Inc., New York.
14. Griffin, T. J., and R. D. Kolodner. 1990. Purification and preliminary characterization of the Escherichia coli K-12 RecF protein. J. Bacteriol. 172: 6291-6299.
15. Groen, B. W., M. A. G. Van Kleef, and J. A. Duine. 1986. Quinohaemoprotein alcohol dehydrogenase apoenzyme from Pseudomonas testosteroni. Biochem. J. 234:611-615.
16. Groman, E. V., and L. L. Engel. 1977. Hydroxysteroid dehydrogenases of Pseudomonas testosteroni: separation of a 17β-hydroxysteroid dehydrogenase from the 3(17)β-hydroxysteroid dehydrogenase and comparison of the two enzymes. Biochim. Biophys. Acta 485:249-254.
17. 4-dehydrogenase from Nocardia corallina: purification and characterization. J. Biochem. 109:581-586.
18. Hayano, M., H. J. Ringold, V. Stefanovic, M. Gut, and R. I. Dorfman. 1961. The stereochemical course of enzymatic steroid 1,2-dehydrogenation. Biochem. Biophys. Res. Commun. 4:454-459.
19. Hopp, T. P., and K. R. Woods. 1981. Predictions of protein antigenic determinants from amino acids sequences. Proc. Natl. Acad. Sci. USA 78:3824-3828.
20. 1-dehydrogenase from Nocardia corallina. J. Biochem. 108:122-127.
21. 1-dehydrogenase from Nacordia corallina. Biochim. Biophys. Acta 1038:60-67.
22. 1,2 dehydrogenation of steroids: kinetics and isotope effects. Biochemistry 4:2113-2126.
23. Kuliopulos, A., D. Shortle, and P. Talalay. 1987. Isolation and sequencing of the gene encoding delta5-3-ketosteroid isomerase of Pseudomonas testosteroni: overexpression of the protein. Proc. Natl. Acad. Sci. USA 84:8893-8897.
24. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
25. Leppik, R. A. 1983. Deoxycholic acid degradation by a Pseudomonas sp.: acidic intermediates with A-ring unsaturation. Biochem. J. 210:829-836.
26. Lestrovaya, N. N., S. Dänhardt, and C. Hörhold. 1978. Reinigung einer 4-en-3-oxosteroid: (akzeptor)-1-en-oxidoreduktase aus N. opaca und charakterisierung der prosthetischen gruppe. Z. Allg. Mikrobiol. 18:189-196.
27. 1-steroid dehydrogenase of Mycobacterium rubrum 121. Izv. Akad. Nauk SSSR Ser. Biol. 5:643-649.
28. Levy, H. R., and P. Talalay. 1957. Enzymatic introduction of double bonds into steroid ring A. J. Am. Chem. Soc. 79:2658-2659.
29. Levy, H. R., and P. Talalay. 1959. Bacterial oxidation of steroids. I. Ring A dehydrogenations by intact cells. J. Biol. Chem. 234:2009-2013.
30. Levy, H. R., and P. Talalay. 1959. Bacterial oxidation of steroids. II. Studies on the enzymatic mechanism of ring A dehydrogenation. J. Biol. Chem. 234:2014-2021.
31. Michaelis, L., and S. Granick. 1943. The polymerization of the free radicals of the Wurster dye type: the dimeric resonance bond. J. Am. Chem. Soc. 65:1747-1755.
32. 5-isomerase and a hypothetical regulatory protein. Mol. Microbiol. 15:895-905.
33. Morales, V. M., A. Bäckman, and M. Bagdasarian. 1991. A series of wide-host-range low-copy-number vectors that allow direct screening for recombinants. Gene 97:39-47.
34. 1,4 unsaturation in microbial transformation of cholesterol. Chem. Pharm. Bull. (Tokyo) 23:2164-2166.
35. Pealing, S. L., A. C. Black, F. D. C. Manson, F. B. Ward, S. K. Chapman, and G. A. Reid. 1992. Sequence of the gene encoding flavocytochrome c from Shewanella putrefaciens: a tetraheme flavoenzyme that is a soluble fumarate reductase related to the membrane-hound enzymes from other bacteria. Biochemistry 31:12132-12140.
36. Philips, G. T., and F. P. Ross. 1974. Stereochemistry of C-1,2-dehydrogenation during cholesterol degradation by Mycobacterium phlei. Eur. J. Biochem. 44:603-610.
37. 1-dehydrogenase (EC 1.3.99.4). J. Bacteriol. 173:7219-7227.
38. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
39. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
40. Smith, A. W., and B. H. Iglewski. 1989. Transformation of Pseudomonas aeruginosa by electroporation. Nucleic Acids Res. 17:10509.
41. Stanier, R. Y., N. J. Palleroni, and M. Doudoroff. 1966. The aerobic pseudomonads: a taxonomic study. J. Gen. Microbiol. 43:159-271.
42. Suzuki, K., S. Ueda, M. Sugiyama, and S. Imamura. 1993. Cloning and expression of a Pseudomonas 3-alpha-hydroxysteroid dehydrogenase-encoding gene in Escherichia coli. Gene 130:137-140.
43. Tabor, S., and C. C. Richardson. 1985. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proc. Natl. Acad. Sci. USA 82:1074-1078.
44. Talalay, P. 1965. Enzymatic mechanisms in steroid biochemistry. Annu. Rev. Biochem. 34:347-380.
45. Talalay, P., and P. I. Marcus. 1956. Specificity, kinetics, and inhibition of α- and β-hydroxysteroid dehydrogenases. J. Biol. Chem. 218:675-691.
46. 5-3-ketosteroids. Biochim. Biophys. Acta 18:300-301.
47. Tazuke, Y., K. Matsuda, K. Adachi, and Y. Tsukada. 1994. Purification and properties of bile acid sulfate sulfatase from Pseudomanas testosteroni. Biosci. Biotechnol. Biochem. 58:889-894.
48. Yanisch-Perron, C., J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13 mp18 and pUC19 vectors. Gene 33:103-119.