Unmarked gene deletion mutagenesis of kstD, encoding 3-ketosteroid Δ1-dehydrogenase, in Rhodococcus erythropolis SQ1 using sacB as counter-selectable marker

FEMS Microbiology Letters

Volumn 205, Issue 2, 2001, Pages 197-202

  Van Der Geize, Robert ^a   Hessels, Gerda I ^a   Van Gerwen, Rick ^b   Van Der Meijden, Peter ^b   Dijkhuizen, Lubbert ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

^b AKZO NOBEL CENTRAL RESEARCH   (Netherlands)

Author keywords

3 Ketosteroid dehydrogenase; Isoenzyme; Rhodococcus; Steroid bioconversion; Unmarked gene deletion

Indexed keywords

ANDROSTENEDIONE; ANDROSTENEDIONE DERIVATIVE; BACTERIAL ENZYME; ISOENZYME; STEROID REDUCTASE;

ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BIOTRANSFORMATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; DEGRADATION; DELETION MUTANT; ENZYME ACTIVITY; ENZYME INACTIVATION; ENZYME SUBSTRATE; ESCHERICHIA COLI; GENE DELETION; GENE ISOLATION; GENE MUTATION; GENE TRANSFER; MARKER GENE; NONHUMAN; PLASMID; PRIORITY JOURNAL; RHODOCOCCUS ERYTHROPOLIS; STOICHIOMETRY; ULTRAVIOLET RADIATION;

GENE DELETION; GENES, REPORTER; GENETIC MARKERS; HEXOSYLTRANSFERASES; MODELS, CHEMICAL; MUTATION; OXIDOREDUCTASES; RHODOCOCCUS;

ESCHERICHIA COLI; RHODOCOCCUS; RHODOCOCCUS ERYTHROPOLIS;

EID: 0035910144     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(01)00464-5     Document Type: Article

References (27)

1. Biotransformations catalyzed by the genus Rhodococcus
2. The genus Rhodococcus
3. Applied aspects of Rhodococcus genetics
4. Identification and mutagenesis by allelic exchange of choE, encoding a cholesterol oxidase from intracellular pathogen Rhodococcus equi
5. Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors
6. Use of rpsL for dominance selection and gene replacement in Streptomyces roseosporus
7. Expression of the Bacillus subtilis sacB gene leads to sucrose sensitivity in the Gram-positive bacterium Corynebacterium glutamicum but not in Streptomyces lividans
8. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: Selection of defined deletions in the chromosome of Corynebacterium glutamicum
9. Unmarked gene integration into the chromosome of Mycobacterium smegmatis via precise replacement of the pyrF gene
10. Gene replacement through homologous recombination in Mycobacterium intracellulare
11. Gene replacement by homologous recombination in Mycobacterium bovis BCG
12. +: A dominant selectable marker for gene replacement in mycobacteria
13. Generation of unmarked directed mutations in mycobacteria, using sucrose counter-selectable suicide vectors
14. Isolation of insertion elements from Gram-positive Brevibacterium, Corynebacterium and Rhodococcus strains using the Bacillus subtilis sacB gene as a positive selection marker
15. Characterization of the basic replicon of Rhodococcus plasmid pSOX and development of a Rhodococcus-Escherichia coli shuttle vector
16. 1-dehydrogenase isoenzyme of Rhodococcus erythropolis strain SQ1
17. Microbial side-chain degradation of sterols
18. Advances in microbial steroid biotransformation
19. Nocardioform arsenic resistance plasmid characterization and improved Rhodococcus cloning vectors
20. A broad host range mobilization system for in vivo genetic engineering: Transposon mutagenesis in Gram negative bacteria
21. Isolation and analysis of mutants of the methylotrophic actinomycete Amycolatopsis methanolica blocked in aromatic amino acid biosynthesis
22. Mutational analysis of primary alcohol metabolism in the methylotrophic actinomycete Amycolatopsis methanolica
23. Molecular characterisation of a Rhodococcus oph operon
24. Illegitimate integration of non-replicative vectors in the genome of Rhodococcus fascians upon electrotransformation as an insertional mutagenesis system
25. A 3-(3-hydroxyphenyl) propionic acid catabolic pathway in Rhodococcus globerulus PWD1: Cloning and characterization of the hpp operon
26. Synthesis of 9α-hydroxysteroids by a Rhodococcus sp