Molecular and functional characterization of kshA and kshB, encoding two components of 3-ketosteroid 9α-hydroxylase, a class IA monooxygenase, in Rhodococcus erythropolis strain SQ1

Molecular Microbiology

Volumn 45, Issue 4, 2002, Pages 1007-1018

  Van Der Geize, R ^a   Hessels, G I ^a   Van Gerwen, R ^b   Van Der Meijden, P ^b   Dijkhuizen, L ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

^b AKZO NOBEL CENTRAL RESEARCH   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

3 KETOSTEROID 9ALPHA HYDROXYLASE A; 3 KETOSTEROID 9ALPHA HYDROXYLASE B; BOLDENONE; OXYGENASE; STEROL; UNCLASSIFIED DRUG; UNSPECIFIC MONOOXYGENASE;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL STRAIN; BACTERIUM MUTANT; CLONING; ENZYMATIC DEGRADATION; ENZYME ACTIVITY; ENZYME SUBSTRATE; ENZYME SUBUNIT; FRAMESHIFT MUTATION; GENE DELETION; GENE IDENTIFICATION; GENETIC CODE; INCUBATION TIME; MOLECULAR BIOLOGY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DOMAIN; RHODOCOCCUS ERYTHROPOLIS;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; DNA PRIMERS; GENE DELETION; GENETIC COMPLEMENTATION TEST; HYDROXYLATION; ISOENZYMES; MOLECULAR SEQUENCE DATA; MUTATION; RHODOCOCCUS; ULTRAVIOLET RAYS;

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); RHODOCOCCUS; RHODOCOCCUS ERYTHROPOLIS; UNCULTURED ACTINOMYCETE;

EID: 0036038941     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2002.03069.x     Document Type: Article

References (40)

1. Basic local alignment search tool
2. Microbial transformation of β-sitosterol and stigmasterol into 26-oxygenated derivatives
3. Phthalate dioxygenase reductase and related flavin-iron-sulfur containing electron transferases
4. Sizing of the Rhodococcus sp. R312 genome by pulsed-field gel electrophoresis. Localization of genes involved in nitrile degradation
5. Cloning and sequencing of Pseudomonas genes encoding vanillate demethylase
6. Mechanisms of steroid oxidation by microorganisms. VII. Properties of the 9α-hydroxylase
7. Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence
8. Phthalate dioxygenase reductase: A modular structure for electron transfer from pyridine nucleotides to [2Fe-2S]
9. Synthesis of 9α-hydroxysteroids by a Rhodococcus sp.
10. Cloning, nucleotide sequence, and expression of the gene encoding a novel dioxygenase involved in metabolism of carboxydiphenyl ethers in Pseudomonas pseudoalcaligenes POB310
11. Role of plasmid pJL1 of Arthrobacter oxydans 317 in the degradation of β-sitosterol
12. 1-Ene-steroid reductase of Mycobacterium sp. NRRL B3805
13. Functional and evolutionary relationships among diverse oxygenases
14. Expression of the Bacillus subtilis sacB gene leads to sucrose sensitivity in the gram-positive bacterium Corynebacterium glutamicum but not in Streptomyces lividans
15. Site-directed mutagenesis of conserved amino acids in the alpha subunit of toluene dioxygenase: Potential mononuclear non-heme iron coordination sites
16. Characterization of the p-toluenesulfonate operon tsaMBCD and tsaR in Comamonas testosteroni T-2
17. Microbial side-chain degradation of sterols
18. Multiple genes encoding 2,3-dihydroxybiphenyl 1,2-dioxygenase in the Gram-positive polychlorinated biphenyl-degrading bacterium Rhodococcus erythropolis TA421, isolated from a termite ecosystem
19. Advances in microbial steroid biotransformation
20. Microbial cleavage of sterol side chains
21. The electron-transport proteins of hydroxylating bacterial dioxygenases
22. Some new intermediates in microbial side chain degradation of β-sitosterol
23. Improved double-stranded DNA sequencing using the linear polymerase chain reaction
24. The nucleotide sequence of the Tn5271 3-chlorobenzoate 3,4-dioxygenase genes (cbaAB) unites the class IA oxygenases in a single lineage
25. Genes in PHT encoding the initial degradation pathway of phthalate in Pseudomonas putida
26. Pulsed-field gel electrophoresis analysis of the genome of Rhodococcus facians: Genome size and linear and circular replicon composition in virulent and avirulent strains
27. Molecular characterization of genes of Pseudomonas sp. strain HR199 involved in bioconversion of vanillin to protocatechuate
28. Nocardioform arsenic resistance plasmid characterization and improved Rhodococcus cloning vectors
29. Chromosome topology and genome size of selected actinomycetes species
30. 2-Oxo-1,2-dihydroquinoline 8-monooxygenase: Phylogenetic relationship to other multicomponent nonheme iron oxygenases
31. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: Selection of defined deletions in the chromosome of Corynebacterium glutamicum
32. A broad host range mobilization system for in vivo genetic engineering: Transposon mutagenesis in gram negative bacteria
33. The steroid 9α-hydroxylation system from Nocardia species
34. Compilation and analysis of DNA sequences associated with apparent streptomycete promoters
35. CLUSTALW: Improving the sensitivity of progressive multiple sequence alignment through sequence weighing, position-specific gap penalties and weight matrix
36. 1-dehydrogenase isoenzyme of Rhodococcus erythropolis strain SQ1
37. 1-dehydrogenase. Rhodococcus erythropolis SQ1 using sacB as counter-selectable marker
38. 1-dehydrogenase isoenzyme
39. Construction of an Escherichia coli-Rhodococcus shuttle vector and plasmid transformation in Rhodococcus spp.
40. Bioconversion of sitosterol to useful steroidal intermediates by mutants of Mycobacterium fortuitum