Simultaneous refolding and purification of recombinant proteins by macro-(affinity ligand) facilitated three-phase partitioning

Analytical Biochemistry

Volumn 430, Issue 1, 2012, Pages 56-64

  Gautam, Saurabh ^a   Dubey, Priyanka ^a   Singh, Pranveer ^b   Varadarajan, Raghavan ^b   Gupta, Munishwar N ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY DELHI   (India)

^b INDIAN INSTITUTE OF SCIENCE   (India)

Author keywords

Antibody fragments; Macro (affinity ligand) facilitated three phase partitioning; Maltose binding protein; Protein refolding; Smart polymers; Three phase partitioning

Indexed keywords

ANTIBODIES; BIOACTIVITY; CIRCULAR DICHROISM SPECTROSCOPY; DICHROISM; ELECTROPHORESIS; EMISSION SPECTROSCOPY; FLUORESCENCE; FUNCTIONAL POLYMERS; LIGANDS; NITROGEN COMPOUNDS; SMART POLYMERS; SODIUM DODECYL SULFATE; SULFUR COMPOUNDS;

AFFINITY LIGANDS; ANTIBODY FRAGMENT; MALTOSE BINDING PROTEINS; PROTEIN REFOLDING; THREE-PHASE PARTITIONING;

RECOMBINANT PROTEINS;

8 ANILINO 1 NAPHTHALENESULFONIC ACID; AMMONIUM SULFATE; CALCIUM ION; GLYCOPROTEIN GP 120; POTASSIUM ION; RECOMBINANT PROTEIN; TERT BUTYL ALCOHOL;

ARTICLE; CIRCULAR DICHROISM; ESCHERICHIA COLI; FLUORESCENCE; FLUORESCENCE SPECTROSCOPY; HYDROPHOBICITY; NONHUMAN; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN REFOLDING; TEMPERATURE;

HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 84865535323     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.07.028     Document Type: Article

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