Refolding of endostatin from inclusion bodies using high hydrostatic pressure

Analytical Biochemistry

Volumn 379, Issue 1, 2008, Pages 32-39

  Chura Chambi, Rosa Maria ^a   Genova, Luis Antonio ^a   Affonso, Regina ^a   Morganti, Ligia ^a  

^a INSTITUTO DE PESQUISAS ENERGÉTICAS E NUCLEARES   (Brazil)

Author keywords

Aggregate; Endostatin; High hydrostatic pressure; Inclusion bodies; Refolding

Indexed keywords

AFFINITY CHROMATOGRAPHY; AGGREGATES; BIOACTIVITY; COLUMN CHROMATOGRAPHY; DICHROISM; ELECTROPHORESIS; ESCHERICHIA COLI; HYDRAULICS; PEPTIDES; SIZE EXCLUSION CHROMATOGRAPHY; SODIUM DODECYL SULFATE; SULFUR COMPOUNDS;

ENDOSTATINS; GUANIDINE HYDROCHLORIDE; HIGH HYDROSTATIC PRESSURE; INCLUSION BODIES; INTRINSIC FLUORESCENCE; REDUCED GLUTATHIONE; REFOLDING; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS;

HYDROSTATIC PRESSURE;

ENDOSTATIN; GLUTATHIONE; GLUTATHIONE DISULFIDE; GUANIDINE; HEPARIN;

AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ARTICLE; BIOLOGICAL ACTIVITY; CELL CULTURE; CELL INCLUSION; CIRCULAR DICHROISM; CONCENTRATION RESPONSE; CONTROLLED STUDY; ESCHERICHIA COLI; FLUORESCENCE; GEL PERMEATION CHROMATOGRAPHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; HYDROSTATIC PRESSURE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN FOLDING; PROTEIN PURIFICATION; WESTERN BLOTTING;

ESCHERICHIA COLI;

EID: 46549085970     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.04.024     Document Type: Article

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