Development of a novel er stress based selection system for the isolation of highly productive clones

Biotechnology and Bioengineering

Volumn 109, Issue 10, 2012, Pages 2599-2611

  Kober, Lars ^a   Zehe, Christoph ^a   Bode, Juergen ^b  

^a Cellca GmbH   (Germany)

^b HANNOVER MEDICAL SCHOOL   (Germany)

Author keywords

Cell specific productivity; Chinese hamster ovary (CHO); ER stress; GRP78; High productive; Recombinant antibody

Indexed keywords

CHINESE HAMSTER OVARY; ER STRESS; GRP78; HIGH PRODUCTIVE; RECOMBINANT ANTIBODY; SPECIFIC PRODUCTIVITY;

CELL CULTURE; CELL MEMBRANES; CLONING; GLUCOSE; MONOCLONAL ANTIBODIES; RECOMBINANT PROTEINS;

CLONE CELLS;

CALRETICULIN; GLUCOSE REGULATED PROTEIN 78; GLUCOSE REGULATED PROTEIN 94; IMMUNOGLOBULIN G ANTIBODY; MESSENGER RNA; X BOX BINDING PROTEIN 1;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL ISOLATION; CHO CELL; CONCENTRATION (PARAMETERS); CORRELATION ANALYSIS; ENDOPLASMIC RETICULUM STRESS; FEMALE; MOLECULAR CLONING; NONHUMAN; PROTEIN EXPRESSION;

ANIMALS; ANTIBODIES, MONOCLONAL; BIOTECHNOLOGY; CELL CULTURE TECHNIQUES; CHO CELLS; CRICETINAE; CRICETULUS; ENDOPLASMIC RETICULUM; GENE EXPRESSION PROFILING; GENES, REPORTER; RECOMBINANT PROTEINS; STRESS, PHYSIOLOGICAL;

CRICETULUS GRISEUS;

EID: 84865356675     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24527     Document Type: Article

References (48)

1. Ahmed SP, Nash RJ, Bridges CG, Taylor DL, Kang MS, Porter EA, Tyms AS. 1995. Antiviral activity and metabolism of the castanospermine derivative MDL 28, 574, in cells infected with herpes simplex virus type 2. Biochem Biophys Res Commun 208(1): 267-273.
2. Back SH, Lee K, Vink E, Kaufman RJ. 2006. Cytoplasmic IRE1alpha-mediated XBP1 mRNA splicing in the absence of nuclear processing and endoplasmic reticulum stress. J Biol Chem 281(27): 18691-18706.
3. Baer AEM. 2002. Funktioneller Vergleich von S/MARs ('scaffold/matrix attachment regions') und Insulatoren im chromosomalen Kontext. PhD Thesis. Technischen Universität Braunschweig.
4. Birch JR, Racher AJ. 2006. Antibody production. Adv Drug Deliv Rev 58(5-6): 671-685.
5. Bole DG, Hendershot LM, Kearney JF. 1986. Posttranslational association of immunoglobulin heavy chain binding protein with nascent heavy chains in nonsecreting and secreting hybridomas. J Cell Biol 102(5): 1558-1566.
6. Borth N, Zeyda M, Kunert R, Katinger H. 2000. Efficient selection of high-producing subclones during gene amplification of recombinant Chinese hamster ovary cells by flow cytometry and cell sorting. Biotechnol Bioeng 71(4): 266-273.
7. Bustin SA. 2000. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. Mol Endocrinol 25(2): 169-193.
8. Calcagno AM, Chewning KJ, Wu CP, Ambubkar SV. 2006. Plasma membrane calcium ATPase (PMCA4): A housekeeper for RT-PCR relative quantification of polytopic membrane proteins. BMC Mol Bio 7: 29.
9. Carroll S, Al-Rubeai M. 2004. The selection of high-producing cell lines using flow cytometry and cell sorting. Expert Opin Biol Ther 4(11): 1821-1829.
10. Chusainow J, Yang YS, Yeo JH, Toh PC, Asvadi P, Wong NS, Yap MG. 2008. A study of monoclonal antibody-producing CHO cell lines: What makes a stable high producer? Biotechnol Bioeng 102(4): 1182-1196.
11. Dübel S. 2007. Recombinant therapeutic antibodies. Appl Mircobiol Biotechnol 74(4): 723-729.
12. El-Hadi M. 2005. Mdg1 und die UPR: Stellung und Funktion des Hsp40-Chaperones in der Unfolded Protein Response. PhD Thesis. Albert-Ludwigs-Universität Freiburg i. Br. Freiburg.
13. Gardner AM, Aviel S, Argon Y. 1993. Rapid degradation of an unassembled immunoglobulin light chain is mediated by a serine protease and occurs in a pre-Golgi compartment. J Biol Chem 268(34): 25940-25947.
14. Gazit G, Kane SE, Nichols P, Lee AS. 1995. Use of the stress-inducible grp78/BiP promoter in targeting high level gene expression in fibrosarcoma in vivo. Cancer Res 55(8): 1660-1663.
15. Haas IG, Wabl M. 1983. Immunoglobulin heavy chain binding protein. Nature 306(5941): 387-389.
16. Hendershot L, Bole D, Köhler G, Kearney JF. 1987. Assembly and secretion of heavy chains that do not associate posttranslationally with immunoglobulin heavy chain-binding protein. J Cell Biol 104(3): 761-767.
17. Jayapal KP, Wlaschin KF, Yap MG, Hu W-S. 2007. Recombinant protein therapeutics from CHO cells-20 years and counting. Chem Eng Prog 103(10): 40-47.
18. Kaloff CR, Haas IG. 1995. Coordination of immunoglobulin chain folding and immunoglobulin chain assembly is essential fort the formation of functional IgG. Immunity 2(6): 629-637.
19. Kaufman RJ. 1999. Stress signaling from the lumen of the endoplasmic reticulum: Coordination of gene transcriptional and translational controls. Genes Dev 13(10): 1211-1233.
20. Knittler MR, Haas IG. 1992. Interaction of BiP with newly synthesized immunoglobulin light chain molecules: Cycles of sequential binding and release. EMBO J 11(4): 1573-1581.
21. Kokame K, Kato H, Miyata T. 2001. Identification of ERSE-II, a new cis-acting element responsible for the ATF6-dependent mammalian unfolded protein response. J Biol Chem 276(12): 9199-9205.
22. Kozutsumi Y, Segal M, Normington K, Gething MJ, Sambrook J. 1988. The presence of malfolded proteins in the endoplasmic reticulum signals the induction of glucose-regulated proteins. Nature 332(6163): 462-464.
23. Lai E, Teodoro T, Volchuk A. 2007. Endoplasmic reticulum stress: Signaling the unfolded protein response. Physiology (Bethesda) 22: 193-201.
24. Lee K. 2002. IRE1-mediated unconventional mRNA splicing and S2P-mediated ATF6 cleavage merge to regulate XBP1 in signaling the unfolded protein response. Genes Dev 16(4): 452-466.
25. Lenny N, Green M. 1991. Regulation of endoplasmic reticulum stress proteins in COS cells transfected with immunoglobulin mu heavy chain cDNA. J Biol Chem 266(30): 20532-20537.
26. 2+ depletion. A comparative analysis with A23187 and the endoplasmic reticulum Ca(2+)-ATPase inhibitor thapsigargin. J Biol Chem 268(16): 12003-12009.
27. Li X, Coffino P. 1993. Degradation of ornithine decarboxylase: Exposure of the C-terminal target by a polyamine-inducible inhibitory protein. Mol Cell Biol 13(4): 2377-2383.
28. 2+ store and inhibition of N-linked glycosylation. Biochem J 318(2): 555-560.
29. Ma Y, Hendershot LM. 2004. Herp is dually regulated by both the endoplasmic reticulum stress-specific branch of the unfolded protein response and a branch that is shared with other cellular stress pathways. J Biol Chem 279(14): 13792-13799.
30. Nakaki T, Deans RJ, Lee AS. 1989. Enhanced transcription of the 78, 000-dalton glucose-regulated protein (GRP78) gene and association of GRP78 with immunoglobulin light chains in a nonsecreting B-cell myeloma line (NS-1). Mol Cell Biol 9(5): 2233-2238.
31. Pahl HL, Baeuerle PA. 1995. A novel signal transduction pathway from the endoplasmic reticulum to the nucleus is mediated by transcription factor NF-κB. EMBO J 14(11): 2580-2588.
32. Pahl HL. 1999. Signal transduction from the endoplasmic reticulum to the cell nucleus. Physiol Rev 79(3): 683-701.
33. Pollard TD, Earnshaw WC. 2007. Cell biology. Berlin: Springer-Verlag. 358-359, 370 p.
34. Qiao J, Oumard A, Wegloehner W, Bode J. 2009. Novel tag-and-exchange (RMCE) strategies generate master cell clones with predictable and stable transgene expression properties. J Mol Biol 390(4): 579-594.
35. Rao RV, Ellerby HM, Bredesen DE. 2004. Coupling endoplasmic reticulum stress to the cell death program. Cell Death Differ 11(4): 372-380.
36. Raymond CS, Soriano P. 2007. High-efficiency FLP and PhiC31 site-specific recombination in mammalian cells. PLoS ONE 2(1): e162.
37. Renna M, Caporaso MG, Bonatti S, Kaufman RJ, Remondelli P. 2007. Regulation of ERGIC-53 gene transcription in response to endoplasmic reticulum stress. J Biol Chem 282(31): 22499-22512.
38. Roy B, Lee AS. 1999. The mammalian endoplasmic reticulum stress response element consists of an evolutionarily conserved tripartite structure and interacts with a novel stress-inducible complex. Nucleic Acids Res 27(6): 1437-1443.
39. Saul R, Ghidoni JJ, Molyneux RJ, Elbein AD. 1985. Castanospermine inhibits alpha-glucosidase activities and alters glycogen distribution in animals. Proc Natl Acad Sci USA 82(1): 93-97.
40. Schlake T, Bode J. 1994. Use of mutated FLP recognition target (FRT) sites for the exchange of expression cassettes at defined chromosomal loci. Biochemistry 33(43): 12746-12751.
41. Schmidt FR. 2004. Recombinant expression systems in the pharmaceutical industry. Appl Microbiol Biotechnol 65(4): 363-372.
42. Shaffer AL, Shapiro-Shelef M, Iwakoshi NN, Lee AH, Qian SB, Zhao H, Yu X, Yang L, Tan BK, Rosenwald A, Hurt EM, Petroulakis E, Sonenberg N, Yewdell JW, Calame K, Glimcher LH, Staudt LM. 2004. XBP1, downstream of Blimp-1, expands the secretory apparatus and other organelles, and increases protein synthesis in plasma cell differentiation. Immunity 21(1): 81-93.
43. Walsh G, Jefferis R. 2006. Post-translational modifications in the context of therapeutic proteins. Nat Biotechnol 24(10): 1241-1252.
44. Wang Y, Shen J, Arenzana N, Tirasophon W, Kaufman RJ, Prywes R. 2000. Activation of ATF6 and an ATF6 DNA binding site by the endoplasmic reticulum stress response. J Biol Chem 275(35): 27013-27020.
45. Winder R. 2005. Cell culture grows capacity; capacity expansions address the 'crunch' for biopharmaceutical manufacturing. Chem Ind 17 Oct: 21-22.
46. Yang Q, Sarnow P. 1997. Location of the internal ribosome entry site in the 5′ non-coding region of the immunoglobulin heavy-chain binding protein (BiP) mRNA: Evidence for specific RNA-protein interactions. Nucleic Acids Res 25(14): 2800-2807.
47. Yoshida H, Haze K, Yanagi H, Yura T, Mori K. 1998. Identification of the cis-acting endoplasmic reticulum stress response element responsible for transcriptional induction of mammalian glucose-regulated proteins. Involvement of basic leucine zipper transcription factors. J Biol Chem 273(50): 33741-33749.
48. Zahn-Zabal M, Kobr M, Girod PA, Imhof M, Chatellard P, de Jesus M, Wurm F, Mermod N. 2001. Development of stable cell lines for production or regulated expression using matrix attachment regions. J Biotechnol 87(1): 29-42.