Combined fish oil and astaxanthin supplementation modulates rat lymphocyte function

European Journal of Nutrition

Volumn 51, Issue 6, 2012, Pages 707-718

  Otton, Rosemari ^a   Marin, Douglas Popp ^a   Bolin, Anaysa Paola ^a   De Cássia Santos MacEdo, Rita ^a   Campoio, Thais Regina ^a   Fineto Jr , Claudio ^a   Guerra, Beatriz Alves ^a   Leite, José Roberto ^b   Barros, Marcelo Paes ^a   Mattei, Rita ^b  

^a UNIVERSIDADE CRUZEIRO DO SUL   (Brazil)

^b FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Antioxidant; Astaxanthin; Carotenoid; Fish oil; Leukocytes; Lymphocyte; N 3 fatty acids; Oxidative stress

Indexed keywords

ASTAREAL A1010; ASTAXANTHIN; CALCIUM ION; DOCOSAHEXAENOIC ACID; FISH OIL; GLUTATHIONE; GLUTATHIONE PEROXIDASE; HYDROGEN PEROXIDE; ICOSAPENTAENOIC ACID; INTERLEUKIN 1BETA; L SELECTIN; NITRIC OXIDE; SUPEROXIDE; SUPEROXIDE DISMUTASE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANTIOXIDANT ACTIVITY; ARTICLE; B LYMPHOCYTE ACTIVATION; BIOSYNTHESIS; CALCIUM CELL LEVEL; CALCIUM TRANSPORT; CELL STRUCTURE; CONTROLLED STUDY; CYTOKINE RELEASE; CYTOSOL; DIET SUPPLEMENTATION; ENZYME ACTIVITY; FAT INTAKE; FOOD COMPOSITION; FOOD DRUG INTERACTION; IMMUNOMODULATION; LYMPH NODE; LYMPHOCYTE PROLIFERATION; MALE; NONHUMAN; NUTRIENT CONTENT; OXIDATION REDUCTION REACTION; OXIDATIVE STRESS; RAT; T LYMPHOCYTE ACTIVATION;

ANIMALS; ANTIOXIDANTS; CALCIUM SIGNALING; CELL PROLIFERATION; CELLS, CULTURED; CYTOKINES; DIETARY SUPPLEMENTS; FISH OILS; GLUTATHIONE; IMMUNOMODULATION; LIPID PEROXIDATION; LYMPHOCYTES; MALE; MITOGENS; NITRIC OXIDE; OXIDATION-REDUCTION; OXIDATIVE STRESS; OXIDOREDUCTASES; RATS; RATS, WISTAR; REACTIVE OXYGEN SPECIES; XANTHOPHYLLS;

ASTA; PISCES; RATTUS;

EID: 84865206956     PISSN: 14366207     EISSN: 14366215     Source Type: Journal    
DOI: 10.1007/s00394-011-0250-z     Document Type: Article

References (78)

1. Calder PC (2009) Polyunsaturated fatty acids and inflammatory processes: new twists in an old tale. Biochimie. doi: 10.1016/j.biochi.2009.01.008
2. PC Calder P Yaqoob 2009 Understanding omega-3 polyunsaturated fatty acids Postgrad Med 121 148 157 10.3810/pgm.2009.11.2083 10.3810/pgm.2009.11.2083
3. PR Fortin RA Lew MH Liang EA Wright LA Beckett TC Chalmers RI Sperling 1995 Validation of a meta-analysis: the effects of fish oil in rheumatoid arthritis J Clin Epidemiol 48 1379 1390 10.1016/0895-4356(95)00028-3 1:STN:280:DyaK28%2FpsFSrsg%3D%3D
4. R De Caterina MI Cybulsky SK Clinton MA Gimbrone Jr P Libby 1994 The omega-3 fatty acid docosahexaenoate reduces cytokine-induced expression of proatherogenic and proinflammatory proteins in human endothelial cells Arterioscler Thromb 14 1829 1836 10.1161/01.ATV.14.11.1829
5. DA Hughes S Southon AC Pinder 1996 (n-3) Polyunsaturated fatty acids modulate the expression of functionally associated molecules on human monocytes in vitro J Nutr 126 603 610 1:CAS:528:DyaK28XhsVyksbw%3D (Pubitemid 26081168)
6. R De Caterina P Libby 1996 Control of endothelial leukocyte adhesion molecules by fatty acids Lipids 31 Suppl S57 S63 10.1007/BF02637052
7. DA Hughes AC Pinder Z Piper IT Johnson EK Lund 1996 Fish oil supplementation inhibits the expression of major histocompatibility complex class II molecules and adhesion molecules on human monocytes Am J Clin Nutr 63 267 272 1:CAS:528:DyaK28XhsFKlsr0%3D (Pubitemid 26036250)
8. PC Calder 2006 Polyunsaturated fatty acids and inflammation Prostaglandins Leukot Essent Fatty Acids 75 197 202 10.1016/j.plefa.2006.05.012 10.1016/j.plefa.2006.05.012 1:CAS:528:DC%2BD28XptFChtrc%3D (Pubitemid 44292527)
9. ES Collie-Duguid KW Wahle 1996 Inhibitory effect of fish oil n-3 polyunsaturated fatty acids on the expression of endothelial cell adhesion molecules Biochem Biophys Res Commun 220 969 974 10.1006/bbrc.1996.0516 10.1006/bbrc.1996.0516 1:CAS:528:DyaK28Xit1KmtLk%3D (Pubitemid 26198623)
10. P Sanderson PC Calder 1998 Dietary fish oil diminishes lymphocyte adhesion to macrophage and endothelial cell monolayers Immunology 94 79 87 10.1046/j.1365-2567.1998.00474.x 1:CAS:528:DyaK1cXjsVagsrg%3D (Pubitemid 28412791)
11. PC Calder P Yaqoob F Thies FA Wallace EA Miles 2002 Fatty acids and lymphocyte functions Br J Nutr 87 Suppl 1 S31 S48 10.1079/BJN2001455 1:CAS:528:DC%2BD38Xht1Whur0%3D
12. Deckelbaum RJ, Calder PC (2010) Dietary n-3 and n-6 fatty acids: are there 'bad' polyunsaturated fatty acids? Curr Opin Clin Nutr Metab Care 13:123-124. doi: 10.1097/MCO.0b013e328336696d
13. MP Barros E Pinto P Colepicolo M Pedersen 2001 Astaxanthin and peridinin inhibit oxidative damage in Fe(2+)-loaded liposomes: scavenging oxyradicals or changing membrane permeability? Biochem Biophys Res Commun 288 225 232 10.1006/bbrc.2001.5765 10.1006/bbrc.2001.5765 1:CAS:528:DC%2BD3MXnsVGntL8%3D (Pubitemid 32999750)
14. P Palozza NI Krinsky 1992 Astaxanthin and canthaxanthin are potent antioxidants in a membrane model Arch Biochem Biophys 297 291 295 10.1016/0003-9861(92)90675-M 1:CAS:528:DyaK38XltVKmsbw%3D (Pubitemid 23282883)
15. T Iwamoto K Hosoda R Hirano H Kurata A Matsumoto W Miki M Kamiyama H Itakura S Yamamoto K Kondo 2000 Inhibition of low-density lipoprotein oxidation by astaxanthin J Atheroscler Thromb 7 216 222 1:CAS:528:DC%2BD3MXktlCmur4%3D
16. T Iwamoto K Hosoda R Hirano H Kurata A Matsumoto W Miki M Kamiyama H Itakura S Yamamoto K Kondo 2000 Inhibition of low-density lipoprotein oxidation by astaxanthin J Atheroscler Thromb 7 216 222 1:CAS:528:DC%2BD3MXktlCmur4%3D
17. LS Jacobsson XM Yuan B Zieden AG Olsson 2004 Effects of alpha-tocopherol and astaxanthin on LDL oxidation and atherosclerosis in WHHL rabbits Atherosclerosis 173 231 237 10.1016/j.atherosclerosis.2004.01.003 1:CAS:528:DC%2BD2cXivVWkt7s%3D (Pubitemid 38469790)
18. K Ohgami K Shiratori S Kotake T Nishida N Mizuki K Yazawa S Ohno 2003 Effects of astaxanthin on lipopolysaccharide-induced inflammation in vitro and in vivo Invest Ophthalmol Vis Sci 44 2694 2701 10.1167/iovs.02-0822 (Pubitemid 36618432)
19. Y Naito K Uchiyama W Aoi G Hasegawa N Nakamura N Yoshida T Maoka J Takahashi T Yoshikawa 2004 Prevention of diabetic nephropathy by treatment with astaxanthin in diabetic db/db mice Biofactors 20 49 59 10.1002/biof.5520200105 1:CAS:528:DC%2BD2cXjtFOmtb0%3D (Pubitemid 38586209)
20. G Hussein U Sankawa H Goto K Matsumoto H Watanabe 2006 Astaxanthin, a carotenoid with potential in human health and nutrition J Nat Prod 69 443 449 10.1021/np050354+ 10.1021/np050354+ 1:CAS:528:DC%2BD2MXhtlarsL3E
21. DM Conroy CD Stubbs J Belin CL Pryor AD Smith 1986 The effects of dietary (n-3) fatty acid supplementation on lipid dynamics and composition in rat lymphocytes and liver microsomes Biochim Biophys Acta 861 457 462 10.1016/0005-2736(86)90454-2 1:CAS:528:DyaL28Xmt1Gis7o%3D (Pubitemid 17183667)
22. R Otton CR Carvalho JR Mendonca R Curi 2002 Low proliferation capacity of lymphocytes from alloxan-diabetic rats: involvement of high glucose and tyrosine phosphorylation of Shc and IRS-1 Life Sci 71 2759 2771 10.1016/S0024-3205(02)02141-0 1:CAS:528:DC%2BD38Xns1SitLw%3D (Pubitemid 35284140)
23. E Pick D Mizel 1981 Rapid microassays for the measurement of superoxide and hydrogen peroxide production by macrophages in culture using an automatic enzyme immunoassay reader J Immunol Methods 46 211 226 10.1016/0022-1759(81) 90138-1 1:CAS:528:DyaL38Xjt12ktA%3D%3D (Pubitemid 11020859)
24. AH Ding CF Nathan DJ Stuehr 1988 Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages. Comparison of activating cytokines and evidence for independent production J Immunol 141 2407 2412 1:CAS:528:DyaL1cXmtV2ns7s%3D
25. R Otton DO da Silva TR Campoio LR Silveira MO de Souza E Hatanaka R Curi 2007 Non-esterified fatty acids and human lymphocyte death: a mechanism that involves calcium release and oxidative stress J Endocrinol 195 133 143 10.1677/JOE-07-0195 10.1677/JOE-07-0195 1:CAS:528:DC%2BD2sXhtlWiu77I (Pubitemid 350056007)
26. 2+ indicators with greatly improved fluorescence properties J Biol Chem 260 3440 3450 1:CAS:528:DyaL2MXitVSmu7s%3D (Pubitemid 15114340)
27. R Otton F Graziola MH Hirata R Curi JF Williams 1998 Dietary fats alter the activity and expression of glucose-6-phosphate dehydrogenase in rat lymphoid cells and tissues Biochem Mol Biol Int 46 529 536 1:CAS:528:DyaK1cXnsFOhsb4%3D (Pubitemid 28508368)
28. H Aebi 1984 Catalase in vitro Methods Enzymol 105 121 126 10.1016/S0076-6879(84)05016-3 1:CAS:528:DyaL2cXltVKis7s%3D
29. B Mannervik 1985 Glutathione peroxidase Methods Enzymol 113 490 495 10.1016/S0076-6879(85)13063-6 1:CAS:528:DyaL28XkslCqtg%3D%3D (Pubitemid 16195820)
30. JF Ewing DR Janero 1995 Microplate superoxide dismutase assay employing a nonenzymatic superoxide generator Anal Biochem 232 243 248 10.1006/abio.1995. 0014 1:CAS:528:DyaK28XhsV2mtQ%3D%3D (Pubitemid 26010266)
31. CG Fraga BE Leibovitz AL Tappel 1988 Lipid peroxidation measured as thiobarbituric acid-reactive substances in tissue slices: characterization and comparison with homogenates and microsomes Free Radic Biol Med 4 155 161 10.1016/0891-5849(88)90023-8 1:CAS:528:DyaL1cXhsVKmtrc%3D (Pubitemid 18052530)
32. B Biteau J Labarre MB Toledano 2003 ATP-dependent reduction of cysteine-sulphinic acid by S. cerevisiae sulphiredoxin Nature 425 980 984 10.1038/nature02075 10.1038/nature02075 1:CAS:528:DC%2BD3sXosVOht7k%3D (Pubitemid 37376931)
33. ME Murphy JP Kehrer 1989 Oxidation state of tissue thiol groups and content of protein carbonyl groups in chickens with inherited muscular dystrophy Biochem J 260 359 364 1:CAS:528:DyaL1MXktVGgtLo%3D (Pubitemid 19151078)
34. I Rahman A Kode SK Biswas 2006 Assay for quantitative determination of glutathione and glutathione disulfide levels using enzymatic recycling method Nat Protoc 1 3159 3165 10.1038/nprot.2006.378 10.1038/nprot.2006.378 1:CAS:528:DC%2BD2sXhtFGjtLzO
35. MM Bradford 1976 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72 248 254 10.1016/0003-2697(76)90527-3 1:CAS:528: DyaE28XksVehtrY%3D
36. PC Calder 2007 Immunological parameters: what do they mean? J Nutr 137 773S 780S 1:CAS:528:DC%2BD2sXisVygsbc%3D (Pubitemid 46397517)
37. Bolin AP, Macedo RC, Marin DP, Barros MP, Otton R (2010) Astaxanthin prevents in vitro auto-oxidative injury in human lymphocytes. Cell Biol Toxicol 26:457-467. doi: 10.1007/s10565-010-9156-4
38. C Pompeia LR Lopes CK Miyasaka J Procopio P Sannomiya R Curi 2000 Effect of fatty acids on leukocyte function Braz J Med Biol Res 33 1255 1268 10.1590/S0100-879X2000001100001 1:CAS:528:DC%2BD3cXos1Ort78%3D
39. H Wang X Xie WG Lu DF Ye HZ Chen X Li Q Cheng 2004 Ovarian carcinoma cells inhibit T cell proliferation: suppression of IL-2 receptor beta and gamma expression and their JAK-STAT signaling pathway Life Sci 74 1739 1749 10.1016/j.lfs.2003.07.051 1:CAS:528:DC%2BD2cXnvVKkug%3D%3D (Pubitemid 38117101)
40. R Gorjao MF Cury-Boaventura TM de Lima R Curi 2007 Regulation of human lymphocyte proliferation by fatty acids Cell Biochem Funct 25 305 315 10.1002/cbf.1388 10.1002/cbf.1388 1:CAS:528:DC%2BD2sXmtVajs7w%3D (Pubitemid 46827126)
41. A Denys A Hichami NA Khan 2005 n-3 PUFAs modulate T-cell activation via protein kinase C-alpha and -epsilon and the NF-kappa B signaling pathway J Lipid Res 46 752 758 10.1194/jlr.M400444-JLR200 10.1194/jlr.M400444-JLR200 1:CAS:528:DC%2BD2MXksFagurs%3D (Pubitemid 43073285)
42. V Bhuvaneswari S Nagini 2005 Lycopene: a review of its potential as an anticancer agent Curr Med Chem Anticancer Agents 5 627 635 10.2174/ 156801105774574667 1:CAS:528:DC%2BD2MXhtFyhtrrF (Pubitemid 41601060)
43. CS Huang MK Shih CH Chuang ML Hu 2005 Lycopene inhibits cell migration and invasion and upregulates Nm23-H1 in a highly invasive hepatocarcinoma, SK-Hep-1 cells J Nutr 135 2119 2123 1:CAS:528:DC%2BD2MXhtVSktbbP
44. Kim YH, Koh HK, Kim DS (2010) Down-regulation of IL-6 production by astaxanthin via ERK-, MSK-, and NF-kappa B-mediated signals in activated microglia. Int Immunopharmacol 10:1560-1572. doi: 10.1016/j.intimp.2010.09.007
45. JM Odeberg P Kaufmann KG Kroon P Hoglund 2003 Lipid drug delivery and rational formulation design for lipophilic drugs with low oral bioavailability, applied to cyclosporine Eur J Pharm Sci 20 375 382 10.1016/j.ejps.2003.08.005 1:CAS:528:DC%2BD3sXptlynuro%3D (Pubitemid 37491431)
46. Y Okada M Ishikura T Maoka 2009 Bioavailability of astaxanthin in Haematococcus algal extract: the effects of timing of diet and smoking habits Biosci Biotechnol Biochem 73 1928 1932 10.1271/bbb.90078 1:CAS:528: DC%2BD1MXht1GhurbO
47. D Petri AK Lundebye 2007 Tissue distribution of astaxanthin in rats following exposure to graded levels in the feed Comp Biochem Physiol C Toxicol Pharmacol 145 202 209 10.1016/j.cbpc.2006.12.008 10.1016/j.cbpc.2006.12.008 (Pubitemid 46341992)
48. TM Razzaq P Ozegbe EC Jury P Sembi NM Blackwell PS Kabouridis 2004 Regulation of T-cell receptor signalling by membrane microdomains Immunology 113 413 426 10.1111/j.1365-2567.2004.01998.x 10.1111/j.1365-2567.2004.01998.x 1:CAS:528:DC%2BD2cXhtVyitbfI (Pubitemid 39593360)
49. T Harder 2004 Lipid raft domains and protein networks in T-cell receptor signal transduction Curr Opin Immunol 16 353 359 10.1016/j.coi.2004.03.013 10.1016/j.coi.2004.03.013 1:CAS:528:DC%2BD2cXjvVamtrs%3D (Pubitemid 38595374)
50. M Wu KA Harvey N Ruzmetov ZR Welch L Sech K Jackson W Stillwell GP Zaloga RA Siddiqui 2005 Omega-3 polyunsaturated fatty acids attenuate breast cancer growth through activation of a neutral sphingomyelinase-mediated pathway Int J Cancer 117 340 348 10.1002/ijc.21238 10.1002/ijc.21238 1:CAS:528: DC%2BD2MXhtFant7rM (Pubitemid 41429556)
51. M Meydani A Azzi 2009 Diabetes risk: antioxidants or lifestyle? Am J Clin Nutr 90 253 254 10.3945/ajcn.2009.28177 10.3945/ajcn.2009.28177 1:CAS:528:DC%2BD1MXpt1Wqs78%3D
52. S Roth W Droge 1987 Regulation of T-cell activation and T-cell growth factor (TCGF) production by hydrogen peroxide Cell Immunol 108 417 424 10.1016/0008-8749(87)90224-3 1:CAS:528:DyaL2sXlsFamsb8%3D (Pubitemid 17126493)
53. SM Keyse RM Tyrrell 1990 Induction of the heme oxygenase gene in human skin fibroblasts by hydrogen peroxide and UVA (365 nm) radiation: evidence for the involvement of the hydroxyl radical Carcinogenesis 11 787 791 10.1093/carcin/11.5.787 1:CAS:528:DyaK3cXkt1Shsr0%3D (Pubitemid 20150246)
54. R Schreck P Rieber PA Baeuerle 1991 Reactive oxygen intermediates as apparently widely used messengers in the activation of the NF-kappa B transcription factor and HIV-1 EMBO J 10 2247 2258 1:CAS:528:DyaK3MXkvVOjurY%3D (Pubitemid 21905698)
55. R Luostarinen T Saldeen 1996 Dietary fish oil decreases superoxide generation by human neutrophils: relation to cyclooxygenase pathway and lysosomal enzyme release Prostaglandins Leukot Essent Fatty Acids 55 167 172 10.1016/S0952-3278(96)90093-9 1:STN:280:DyaK2s%2FosVOrsg%3D%3D (Pubitemid 26366834)
56. M Fisher PH Levine BH Weiner MH Johnson EM Doyle PA Ellis JJ Hoogasian 1990 Dietary n-3 fatty acid supplementation reduces superoxide production and chemiluminescence in a monocyte-enriched preparation of leukocytes Am J Clin Nutr 51 804 808 1:CAS:528:DyaK3cXktl2hu7Y%3D
57. Tayyebi-Khosroshahi H, Houshyar J, Tabrizi A, Vatankhah AM, Razzagi Zonouz N, Dehghan-Hesari R (2010) Effect of omega-3 fatty acid on oxidative stress in patients on hemodialysis. Iran J Kidney Dis 4(4):322-326
58. Kim SJ, Zhang Z, Saha A, Sarkar C, Zhao Z, Xu Y, Mukherjee AB (2010) Omega-3 and omega-6 fatty acids suppress ER- and oxidative stress in cultured neurons and neuronal progenitor cells from mice lacking PPT1. Neurosci Lett 479:292-296. doi: 10.1016/j.neulet.2010.05.083
59. W Komatsu K Ishihara M Murata H Saito K Shinohara 2003 Docosahexaenoic acid suppresses nitric oxide production and inducible nitric oxide synthase expression in interferon-gamma plus lipopolysaccharide-stimulated murine macrophages by inhibiting the oxidative stress Free Radic Biol Med 34 1006 1016 10.1016/S0891-5849(03)00027-3 1:CAS:528:DC%2BD3sXislemtrY%3D (Pubitemid 36398560)
60. G Lu EL Greene T Nagai BM Egan 1998 Reactive oxygen species are critical in the oleic acid-mediated mitogenic signaling pathway in vascular smooth muscle cells Hypertension 32 1003 1010 10.1161/01.HYP.32.6.1003 1:CAS:528: DyaK1MXitVWjtA%3D%3D (Pubitemid 29001427)
61. DS Kelley NE Hubbard KL Erickson 2005 Regulation of human immune and inflammatory responses by dietary fatty acids Adv Food Nutr Res 50 101 138 10.1016/S1043-4526(05)50004-4 10.1016/S1043-4526(05)50004-4 1:CAS:528: DC%2BD1cXitlKhsL8%3D (Pubitemid 43588658)
62. K Fritsche 2006 Fatty acids as modulators of the immune response Annu Rev Nutr 26 45 73 10.1146/annurev.nutr.25.050304.092610 10.1146/annurev.nutr.25. 050304.092610 1:CAS:528:DC%2BD28Xpt1Clsbo%3D
63. Lecchi C, Invernizzi G, Agazzi A, Ferroni M, Pisani LF, Savoini G, Ceciliani F (2011) In vitro modulation of caprine monocyte immune functions by omega-3 polyunsaturated fatty acids. Vet J 189(3):353-355. doi: 10.1016/j.tvjl.2010.09.001
64. AR Guimaraes R Curi 1991 Metabolic changes induced by w-3 polyunsaturated fatty acid rich-diet (w-3 PUFA) on the thymus, spleen and mesenteric lymph nodes of adult rats Biochem Int 25 689 695 1:CAS:528:DyaK38Xot1Knug%3D%3D
65. NI Krinsky EJ Johnson 2005 Carotenoid actions and their relation to health and disease Mol Aspects Med 26 459 516 10.1016/j.mam.2005.10.001 10.1016/j.mam.2005.10.001 1:CAS:528:DC%2BD2MXht1OnurfL (Pubitemid 41741836)
66. M Nakano A Onodera E Saito M Tanabe K Yajima J Takahashi VC Nguyen 2008 Effect of astaxanthin in combination with alpha-tocopherol or ascorbic acid against oxidative damage in diabetic ODS rats J Nutr Sci Vitaminol 54 329 334 10.3177/jnsv.54.329 1:CAS:528:DC%2BD1cXhtV2ju7fN
67. M Ikeuchi T Koyama J Takahashi K Yazawa 2007 Effects of astaxanthin in obese mice fed a high-fat diet Biosci Biotechnol Biochem 71 893 899 10.1271/bbb.60521 1:CAS:528:DC%2BD2sXltFCnsL8%3D (Pubitemid 46650940)
68. YM Naguib 2000 Antioxidant activities of astaxanthin and related carotenoids J Agric Food Chem 48 1150 1154 10.1021/jf991106k 1:CAS:528:DC%2BD3cXhvVyrsro%3D (Pubitemid 30243677)
69. S Brechard EJ Tschirhart 2008 Regulation of superoxide production in neutrophils: role of calcium influx J Leukoc Biol 84 1223 1237 10.1189/jlb.0807553 10.1189/jlb.0807553 1:CAS:528:DC%2BD1cXhtlWgu7jO
70. Prasad A, Bloom MS, Carpenter DO (2010) Role of calcium and ROS in cell death induced by polyunsaturated fatty acids in murine thymocytes. J Cell Physiol 225:829-836. doi: 10.1002/jcp.22290
71. Yog R, Barhoumi R, McMurray DN, Chapkin RS (2010) n-3 polyunsaturated fatty acids suppress mitochondrial translocation to the immunologic synapse and modulate calcium signaling in T cells. J Immunol 184(10):5865-5873. doi: 10.4049/jimmunol.0904102
72. Lin TY, Lu CW, Wang SJ (2010) Astaxanthin inhibits glutamate release in rat cerebral cortex nerve terminals via suppression of voltage-dependent Ca(2+) entry and mitogen-activated protein kinase signaling pathway. J Agric Food Chem 58:8271-8278. doi: 10.1021/jf101689t
73. Macedo RC, Bolin AP, Marin DP, Otton R (2010) Astaxanthin addition improves human neutrophils function: in vitro study. Eur J Nutr 49:447-457. doi: 10.1007/s00394-010-0103-1
74. Maoka T, Etoh T, Kishimoto S, Sakata S (2011) Carotenoids and their fatty acid esters in the petals of Adonis aestivalis. J Oleo Sci 60:47-52
75. TH Wu JH Liao WC Hou FY Huang TJ Maher CC Hu 2006 Astaxanthin protects against oxidative stress and calcium-induced porcine lens protein degradation J Agric Food Chem 54 2418 2423 10.1021/jf052651q76 10.1021/jf052651q 1:CAS:528:DC%2BD28XhsVSqtbs%3D
76. A Hirasawa K Tsumaya T Awaji S Katsuma T Adachi M Yamada Y Sugimoto S Miyazaki G Tsujimoto 2005 Free fatty acids regulate gut incretin glucagon-like peptide-1 secretion through GPR120 Nat Med 11 90 94 10.1038/nm1168 10.1038/nm1168 1:CAS:528:DC%2BD2MXptVSi (Pubitemid 40215843)
77. LJ Picker EC Butcher 1992 Physiological and molecular mechanisms of lymphocyte homing Annu Rev Immunol 10 561 591 10.1146/annurev.iy.10.040192. 003021 10.1146/annurev.iy.10.040192.003021 1:CAS:528:DyaK38XltV2ntLc%3D
78. C Mora IS Grewal FS Wong RA Flavell 2004 Role of l-selectin in the development of autoimmune diabetes in non-obese diabetic mice Int Immunol 16 257 264 10.1093/intimm/dxh036 1:CAS:528:DC%2BD2cXmtl2isA%3D%3D (Pubitemid 38195656)