Segmental isotopic labeling of a 140 kDa dimeric multi-domain protein CheA from Escherichia coli by expressed protein ligation and protein trans-splicing

Journal of Biomolecular NMR

Volumn 53, Issue 3, 2012, Pages 191-207

  Minato, Yuichi ^a   Ueda, Takumi ^a   MacHiyama, Asako ^a   Shimada, Ichio ^a,b   Iwaï, Hideo ^c  

^a UNIVERSITY OF TOKYO   (Japan)

^b NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^c UNIVERSITY OF HELSINKI   (Finland)

Author keywords

CheA; Expressed protein ligation; Intein; Protein splicing; Protein trans splicing; Segmental isotopic labeling

Indexed keywords

CHEA KINASE;

ARTICLE; ESCHERICHIA COLI; EXPRESSED PROTEIN LIGATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN INDUCTION, SYNTHESIS AND MODIFICATION; PROTEIN TRANS SPLICING;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BIOCHEMISTRY; CLONING, MOLECULAR; ISOTOPE LABELING; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; NITROGEN ISOTOPES; PROTEIN MULTIMERIZATION; PROTEIN SPLICING;

ESCHERICHIA COLI;

EID: 84865179759     PISSN: 09252738     EISSN: 15735001     Source Type: Journal    
DOI: 10.1007/s10858-012-9628-3     Document Type: Article

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