Expressed protein ligation for a large dimeric protein

Protein Engineering, Design and Selection

Volumn 24, Issue 6, 2011, Pages 495-501

  Karagöz, G Elif ^a   Sinnige, Tessa ^a   Hsieh, Ofey ^a,b   Rdiger, Stefan G D ^a  

^a UTRECHT UNIVERSITY   (Netherlands)

^b UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

intein; molecular chaperones; protein engineering; protein folding; proteinprotein interactions

Indexed keywords

BIOPHYSICAL APPLICATIONS; C-TERMINAL FRAGMENTS; DIMERIC PROTEINS; EXPRESSED PROTEIN LIGATION; FLEXIBLE SEGMENTS; INTEIN; ISOTOPE LABELLING; MOLECULAR CHAPERONES; OLIGOMERIC PROTEINS; PEPTIDE FRAGMENTS; PROTEIN ENGINEERING; PROTEIN-PROTEIN INTERACTIONS; REACTION CONDITIONS; UPSCALING;

BIOCHEMICAL ENGINEERING; DIMERS; FLUORESCENCE SPECTROSCOPY; GENETIC ENGINEERING; ISOTOPES; NUCLEAR MAGNETIC RESONANCE; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PROTEIN FOLDING; RESONANCE;

PROTEINS;

CHAPERONE; DIMER; HEAT SHOCK PROTEIN 90;

AMINO TERMINAL SEQUENCE; ARTICLE; CHROMATOGRAPHY; ISOTOPE LABELING; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN EXPRESSION;

ELECTROPHORESIS, POLYACRYLAMIDE GEL; HSP90 HEAT-SHOCK PROTEINS; HUMANS; HYDROGEN-ION CONCENTRATION; INTEINS; MODELS, MOLECULAR; PROTEIN ENGINEERING; PROTEIN FOLDING; PROTEIN INTERACTION DOMAINS AND MOTIFS; PROTEIN MULTIMERIZATION;

EID: 79956150267     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzr007     Document Type: Article

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