Improved Phos-tag SDS-PAGE under neutral pH conditions for advanced protein phosphorylation profiling

Proteomics

Volumn 11, Issue 2, 2011, Pages 319-323

  Kinoshita, Eiji ^a   Kinoshita Kikuta, Emiko ^a  

^a HIROSHIMA UNIVERSITY   (Japan)

Author keywords

Bis tris; Phos tag; Phosphoprotein; Phosphorylation; SDS PAGE; Technology

Indexed keywords

ACRYLAMIDE; BETA CATENIN; BUFFER; MANGANESE; PEPSIN A; PHOSPHATE; PHOSPHOPROTEIN; POLYACRYLAMIDE; PROTEIN; PROTEIN TYROSINE KINASE; TAU PROTEIN; ZINC;

ARTICLE; CELL LYSATE; GEL; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; RELIABILITY;

ANIMALS; BETA CATENIN; CELL LINE, TUMOR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; HYDROGEN-ION CONCENTRATION; PEPSIN A; PHOSPHOPROTEINS; PHOSPHORYLATION; RECOMBINANT PROTEINS; SWINE; TAU PROTEINS;

PHOS;

EID: 78650790948     PISSN: 16159853     EISSN: 16159861     Source Type: Journal    
DOI: 10.1002/pmic.201000472     Document Type: Article

References (16)

1. Kinoshita, E., Kinoshita-Kikuta, E., Takiyama, K., Koike, T., Phosphate-binding tag, a new tool to visualize phosphorylated proteins. Mol. Cell. Proteomics 2006, 5, 749-757.
2. Kinoshita-Kikuta, E., Aoki, Y., Kinoshita, E., Koike, T., Label-free kinase profiling using phosphate affinity polyacrylamide gel electrophoresis. Mol. Cell. Proteomics 2007, 6, 356-366.
3. Kinoshita, E., Kinoshita-Kikuta, E., Ujihara, H., Koike, T., Mobility shift detection of phosphorylation on large proteins using a Phos-tag SDS-PAGE gel strengthened with agarose. Proteomics 2009, 9, 4098-4101.
4. Kinoshita, E., Kinoshita-Kikuta, E., Koike, T., Separation and detection of large phosphoproteins using Phos-tag SDS-PAGE. Nat. Protoc. 2009, 4, 1513-1521.
5. Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227, 680-685.
6. Kinoshita, E., Kinoshita-Kikuta, E., Koike, T., Phosphate-affinity gel electrophoresis using a Phos-tag molecule for phosphoproteome study. Curr. Proteomics 2009, 6, 104-121.
7. Kinoshita, E., Kinoshita-Kikuta, E., Matsubara, M., Yamada, S. et al., Separation of phosphoprotein isotypes having the same number of phosphate groups using phosphate-affinity SDS-PAGE. Proteomics 2008, 8, 2994-3003.
8. Lee, D., Ryle, A. P., Pepsin, D., A minor component of commercial pepsin preparations. Biochem. J. 1967, 104, 742-748.
9. Foltmann, B., Drøhse, H. B., Nielsen, P. K., James, M. N., Separation of porcine pepsinogen A and progastricsin. Sequencing of the first 73 amino acid residues in progastricsin. Biochim. Biophys. Acta 1992, 1121, 75-82.
10. Fu, J. Y., Wu, S. M., Chang, C. T., Sung, H. Y., Characterization of three chitosanase isozymes isolated from a commercial crude porcine pepsin preparation. J. Agric. Food Chem. 2003, 51, 1042-1048.
11. Lebouvier, T., Scales, T. M., Williamson, R., Noble, W. et al., The microtubule-associated protein tau is also phosphorylated on tyrosine. J. Alzheimer Dis. 2009, 18, 1-9.
12. Lilien, J., Balsamo, J., The regulation of cadherin-mediated adhesion by tyrosine phosphorylation/dephosphorylation of β-catenin. Curr. Opin. Cell Biol. 2005, 17, 459-465.
13. Kikuchi, A., Tumor formation by genetic mutations in the components of the Wnt signaling pathway. Cancer Sci. 2003, 94, 225-229.
14. Taurin, S., Sandbo, N., Qin, Y., Browning, D. et al., Phosphorylation of β-catenin by cyclic AMP-dependent protein kinase. J. Biol. Chem. 2006, 281, 9971-9976.
15. Fang, D., Hawke, D., Zheng, Y., Xia, Y. et al., Phosphorylation of β-catenin by AKT promotes β-catenin transcriptional activity. J. Biol. Chem. 2007, 282, 11221-11229.
16. Kinoshita, E., Kinoshita-Kikuta, E., Matsubara, M., Aoki, Y. et al., Two-dimensional phosphate-affinity gel electrophoresis for the analysis of phosphoprotein isotypes. Electrophoresis 2009, 30, 550-559.