Harvesting and cryo-cooling crystals of membrane proteins grown in lipidic mesophases for structure determination by macromolecular crystallography

Journal of Visualized Experiments

Volumn , Issue 67, 2012, Pages

  Li, Dianfan ^a   Boland, Coilín ^a   Aragao, David ^a   Walsh, Kilian ^a   Caffrey, Martin ^a  

^a TRINITY COLLEGE DUBLIN   (Ireland)

Author keywords

Crystallization; Glass sandwich plates; GPCR; Harvesting; In meso; Issue 67; LCP; Lipidic mesophases; Macromolecular X ray crystallography; Membrane protein

Indexed keywords

GLASS; LIPID; MEMBRANE PROTEIN;

ARTICLE; CHEMISTRY; COLD; CRYSTALLIZATION; INSTRUMENTATION; METHODOLOGY; X RAY CRYSTALLOGRAPHY;

COLD TEMPERATURE; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; GLASS; LIPIDS; MEMBRANE PROTEINS;

EID: 84865146809     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/4001     Document Type: Article

References (34)

1. Caffrey, M. Crystallizing membrane proteins for structure determination: use of lipidic mesophases. Annu. Rev. Biophys. 38, 29-51 (2009).
2. Caffrey, M., Li, D., & Dukkipati, A. Membrane protein structure determination using crystallography and lipidic mesophases - recent advances and successes. Biochemistry. DOI: 10.1021/bj300010w (In Press, 2012).
3. Caffrey, M. & Cherezov, V. Crystallizing membrane proteins using lipidic mesophases. Nat. Protocols. 4, 706-731 (2009).
4. Cherezov, V., Clogston, J., Papiz, M.Z., & Caffrey, M. Room to move: crystallizing membrane proteins in swollen lipidic mesophases. J. Mol. Biol. 357, 1605-1618 (2006).
5. Cherezov, V., Peddi, A., Muthusubramaniam, L., Zheng, Y.F., & Caffrey, M. A robotic system for crystallizing membrane and soluble proteins in lipidic mesophases. Acta Crystallogr. D. 60, 1795-1807 (2004).
6. Cherezov, V., Rosenbaum, D.M., Hanson, M.A., Rasmussen, S.G., Thian, F.S., Kobilka, T.S., Choi, H.J., Kuhn, P., Weis, W.I., Kobilka, B.K., & Setvens, R.C. High-resolution crystal structure of an engineered human beta2-adrenergic G protein-coupled receptor. Science. 318, 1258-1265 (2007).
7. Chien, E.Y., Liu, W., Zhao, Q., Katritch, V., Han, G.W., Hanson, M.A., Shi, L., Newman, A.H., Javitch, J.A., Cherezov, V., & Stevens, R.C. Structure of the human dopamine D3 receptor in complex with a D2/D3 selective antagonist. Science. 330, 1091-1095 (2010).
8. Granier, S., Manglik, A., Kruse, A.C., Kobilka, T.S., Thian, F.S, Weis, W.I., & Kobilka, B.K. Structure of the delta-opioid receptor bound to naltrindole. Nature. 485, 400-404 (2012).
9. Haga, K., Kruse, A.C., Asada, H., Yurugi-Kobayashi, T., Shiroishi, M., Zhang, C., Weis, W.I., Okada, T., Kobilka, B.K., Haga, T., & Kobayashi, T. Structure of the human M2 muscarinic acetylcholine receptor bound to an antagonist. Nature. 482, 547-551 (2012).
10. Hanson, M.A., Roth, B., Jo, E., Griffith, M.T., Scott, F.L., Reinhart, G., Desale, H., Clemons, B., Cahalan, S.M., Schuerer, S.C., Sanna, M.G., Han, G.W., Kuhn, P., Rosen, H., & Stevens, R.C. Crystal structure of a lipid G protein-coupled receptor. Science. 335, 851-855 (2012).
11. Jaakola, V.P., Griffith, M.T., Hanson, M.A., Cherezov, V., Chien, E.Y., Lane, J.R., Ijzerman, A.P., & Stevens, R.C. The 2.6 angstrom crystal structure of a human A2A adenosine receptor bound to an antagonist. Science. 322, 1211-1217 (2008).
12. Kruse, A.C., Hu, J., Pan, A.C., Arlow, D.H., Rosenbaum, D.M., Rosemond, E., Green, H.F., Liu, T., Chae, P.S., Dror, R.O., Shaw, D.E., Weis, W.I., Wess, J., & Kobilka, B.K. Structure and dynamics of the M3 muscarinic acetylcholine receptor. Nature. 482, 552-556 (2012).
13. Manglik, A., Kruse, A.C., Kobilka, T.S., Thian, F.S., Mathiesen, J.M., Sunahara, R.K., Pardo, L., Weis, W.I., Kobilka, B.K., & Granier, S. Crystal structure of the micro-opioid receptor bound to a morphinan antagonist. Nature. 485, 321-326 (2012).
14. Rasmussen, S.G., Choi, H.J., Fung, J.J., Pardon, E., Casarosa, P., Chae, P.S., Devree, B.T., Rosenbaum, D.M., Thian, F.S., Kobilka, T.S., Schnapp, A., Konetzki, I., Sunahara, R.K., Gellman, S.H., Pautsch, A., Steyaert, J., Weis, W.I., & Kobilka, B.K. Structure of a nanobodystabilized active state of the beta(2) adrenoceptor. Nature. 469, 175-180 (2011).
15. Rasmussen, S G.F., Devree, B.T., Zou, Y., Kruse, A.C., Chung, K.Y., Kobilka, T.S., Thian, F.S., Chae, P.S., Pardon, E., Calinski, D., Mathiesen, J.M., Shah, S.T., Lyons, J.A., Caffrey, M., Gellman, S.H., Steyaert, J., Skiniotis, G., Weis, W.I., Sunahara, R.K., & Kobilka, B.K. Crystal structure of the β2 adrenergic receptor-Gs protein complex. Nature. 477, 549-555 (2011).
16. Rosenbaum, D.M., Cherezov, V., Hanson, M.A., Rasmussen, S.G., Thian, F.S., Kobilka, T.S., Choi, H.J., Yao, X.J., Weis, W.I., Stevens, R.C., & Kobilka, B.K. GPCR engineering yields high-resolution structural insights into beta2-adrenergic receptor function. Science. 318, 1266-1273 (2007).
17. Rosenbaum, D.M., Zhang, C., Lyons, J.A., Holl, R., Aragao, D., Arlow, D.H., Rasmussen, S.G., Choi, H.J., Devree, B.T., Sunahara, R.K., Chae, P.S., Gellman, S.H., Dror, R.O., Shaw, D.E., Weis, W.I., Caffrey, M., Gmeiner, P., & Kobilka, B.K Structure and function of an irreversible agonist-beta(2) adrenoceptor complex. Nature. 469, 236-240 (2011).
18. Shimamura, T., Shiroishi, M., Weyand, S., Tsujimoto, H., Winter, G., Katritch, V., Abagyan, R., Cherezov, V., Liu, W., Han, G.W., Kobayashi, T., Setvens, R.C., & Iwata, S. Structure of the human histamine H1 receptor complex with doxepin. Nature. 475, 65-70 (2011).
19. Thompson, A.A., Liu, W., Chun, E., Katritch, V., We, H., Vardy, E., Huang, X.P., Trapella, C., Guerrini, R., Calo, G., Roth, B.L., Cherezov, V., & Stevens, R.C. Structure of the nociceptin/orphanin FQ receptor in complex with a peptide mimetic. Nature. 485, 395-399 (2012).
20. Wu, B., Chien, E.Y., Mol, C.D., Fenalti, G., Liu, W., Katritch, V., Abagyan, R., Brooun, A., Wells, P., Bi, F.C., Hamel, D.J., Kuhn, P., Handel, T.M., Cherezov, V., & Stevens, R.C. Structures of the CXCR4 Chemokine GPCR with Small-Molecule and Cyclic Peptide Antagonists. Science. 330, 1066 -1071 (2010).
21. Wu, H., Wacker, D., Mileni, M., Katritch, V., Han, G.W., Vardy, E., Liu, W., Thompson, A.A., Huang, X.P., Carroll, F.I., Mascarella, S.W., Westkaemper, R.B., Mosier, P.D., Roth, B.L., Cherezov, V., & Stevens, R.C. Structure of the human kappa-opioid receptor in complex with JDTic. Nature. 485, 327-332 (2012).
22. Cherezov, V., Abola, E. & Stevens, R.C. Recent progress in the structure determination of GPCRs, a membrane protein family with high potential as pharmaceutical targets. Methods Mol. Biol. 654, 141-168 (2010).
23. Liu, W. & Cherezov, V. Crystallization of membrane proteins in lipidic mesophases. J. Vis. Exp. (2011).
24. Cherezov, V. & Caffrey, M. Nano-volume plates with excellent optical properties for fast, inexpensive crystallization screening of membrane proteins. J. Appl. Cryst. 36, 1372-1377 (2003).
25. Cherezov, V. & Caffrey, M. Picolitre-scale crystallization of membrane proteins. J. Appl. Cryst. 39, 604-606 (2006).
26. Wöhri, A.B., Johansson, L.C., Wadsten-Hindrichsen, P., Wahlgren, W.Y., Fischer, G., Horsefield, R., Katona, G., Nyblom, M., Oberg, F., Young, G., Cogdell, R.J., Fraser, N.J., Engstrom, S., & Neutze, R. A Lipidic-Sponge Phase Screen for Membrane Protein Crystallization. Structure. 16, 1003-1009 (2008).
27. Cherezov, V., et al. Rastering strategy for screening and centring of microcrystal samples of human membrane proteins with a sub-10 microm size X-ray synchrotron beam. J. R. Soc. Interface. 6 Suppl 5, S587-597 (2009).
28. Caffrey, M.A lipid's eye view of membrane protein crystallization in mesophases. Curr. Opin. Struct. Biol. 10, 486-497 (2000).
29. Briggs, J., Chung, H. & Caffrey, M. The temperature-composition phase diagram and mesophase structure characterization of the monoolein/ water system. J. Phys. Ii. 6, 723-751 (1996).
30. Qiu, H. & Caffrey, M. The phase diagram of the monoolein/water system: metastability and equilibrium aspects. Biomaterials. 21, 223-234 (2000).
31. Misquitta, Y., Cherezov, V., Havas, F., Patterson, S., Mohan, J.M., Wells, A.J., Hart, D.J., & Caffrey, M. Rational design of lipid for membrane protein crystallization. J. Struct. Biol. 148, 169-175 (2004).
32. Caffrey, M. & Porter, C. Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases. J. Vis. Exp. e1712 (2010).
33. Li, D., Boland, C., Walsh, K., & Caffrey, M. Use of a robot for high-throughput crystallization of membrane proteins in lipidic mesophase. J. Vis. Exp. e4000 (2012).
34. Lyons, J.A., Aragao, D., Slattery, O., Pisliakov, A.V., Soulimane, T., & Caffrey, M. Structure insights into electron transfer in caa(3)-type cytochrome oxidase. Nature. DOI: 10.1038/nature11182 (2012).