Crystallizing membrane proteins for structure determination using lipidic mesophases

Journal of Visualized Experiments

Volumn , Issue 45, 2010, Pages

  Caffrey, Martin ^a   Porter, Christopher ^a  

^a TRINITY COLLEGE DUBLIN   (Ireland)

Author keywords

Biochemistry; Crystallization; In meso; Issue 45; Lipidic mesophases; Manual; Membrane; Membrane protein

Indexed keywords

EID: 80055110454     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1712     Document Type: Article

References (9)

1. Caffrey, M. Crystallizing membrane proteins for structure determination. Use of lipidic mesophases. Annu. Rev. Biophys. 38, 29-51 (2009).
2. Caffrey, M. & Cherezov, V. Crystallising membrane proteins in lipidic mesophases. Nat. Protoc. 4, 706-731 (2009).
3. Cheng, A., Hummel, B., Qiu, H. & Caffrey, M. A simple mechanical mixer for small viscous lipid-containing samples. Chem. Phys. Lipids 95, 11-21 (1998).
4. Cherezov, V. & Caffrey, M. Nano-volume plates with excellent optical properties for fast, inexpensive crystallization screening of membrane proteins. J. Appl. Cryst. 36, 1372-1377 (2003).
5. Qiu, H. & Caffrey, M. The phase diagram of the monoolein/water system: metastability and equilibrium aspects. Biomaterials 21, 223-234 (2000).
6. Cherezov, V., et al. In meso structure of the cobalamin transporter, BtuB, at 1. 95 A resolution. J. Mol. Biol. 364, 716-734 (2006).
7. Cherezov, V., Clogston, J., Papiz, M.Z. & Caffrey, M. Room to move: crystallizing membrane proteins in swollen lipidic mesophases. J. Mol. Biol. 357, 1605-1618 (2006).
8. Cherezov, V., et al. In meso crystal structure and docking simulations suggest an alternative proteoglycan binding site in the OpcA outer membrane adhesin. Proteins 71, 24-34 (2008).
9. Cherezov, V., Peddi, A., Muthusubramaniam, L., Zheng, Y.F. & Caffrey, M. A robotic system for crystallizing membrane and soluble proteins in lipidic mesophases. Acta Crystallogr. D Biol. Crystallogr. 60, 1795-1807 (2004).