Crystallization of membrane proteins in lipidic mesophases

Journal of Visualized Experiments

Volumn , Issue 49, 2011, Pages

  Liu, Wei ^a   Cherezov, Vadim ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80055115291     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2501     Document Type: Article

References (13)

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2. Cherezov, V., Abola, E. & Stevens, R.C. Recent progress in the structure determination of GPCRs, a membrane protein family with high potential as pharmaceutical targets. Methods Mol. Biol. 654, 141-170 (2010).
3. Cherezov, V., Liu, J., Hanson, M.A., Griffith, M.T. & Stevens, R.C. LCP-FRAP assay for pre-screening membrane proteins for in meso crystallization. J. Cryst. Growth Design 8, 4307-4315 (2008).
4. Misquitta, Y. & Caffrey, M. Detergents destabilize the cubic phase of monoolein: implications for membrane protein crystallization. Biophys. J. 79, 394-405 (2003).
5. Soumpasis, D.M. Theoretical analysis of fluorescence photobleaching recovery experiments. Biophys. J. 41, 95-97 (1983).
6. Cherezov, V., Hanson, M.A., Griffith, M.T., Hilgart, M.C., Sanishvili, R., Nagarajan, V., Stepanov, S., Fischetti, R.F., Kuhn, P. & Stevens, R.C. Rastering strategy for screening and centering of microcrystal samples of human membrane proteins with a sub-10 micrometer size X-ray synchrotron beam. J. R. Soc. Interface 6, Suppl. 5, S587-S597 (2009).
7. Cherezov, V., Rosenbaum, D.M., Hanson, M.A., Rasmussen, S.G., Thian, F.S., Kobilka, T.S., Choi, H.J., Kuhn, P., Weis, W.I., Kobilka, B.K. & Stevens, R.C. High-resolution crystal structure of an engineered human beta2-adrenergic G protein-coupled receptor. Science 318, 1258-1265 (2007).
8. Cherezov, V., Peddi, A., Muthusubramaniam, L., Zheng, Y.F. & Caffrey, M. A robotic system for crystallizing membrane and soluble proteins in lipidic mesophases. Acta Crystallogr. D 60, 1795-1807 (2004).
9. Kissick, D.J., Gualtieri, E.J., Simpson, G.J. & Cherezov, V. Nonlinear optical imaging of integral membrane protein crystals in lipidic mesophases. Anal. Chem. 82, 491-497 (2010).
10. Cheng, A., Hummel, B., Qiu, H., & Caffrey, M. A simple mechanical mixer for small viscous lipid-containing samples. Chem. Phys. Lipids 95, 11-21 (1998).
11. Cherezov, V. & Caffrey, M. A simple and inexpensive nanoliter-volume dispenser for highly viscous materials used in membrane protein crystallization. J. Appl. Cryst. 38, 398-400 (2005).
12. Cherezov, V. & Caffrey, M. Nano-volume plates with excellent optical properties for fast, inexpensive crystallization screening of membrane proteins. J. Appl. Cryst. 36, 1372-1377 (2003).
13. Cherezov, V., Fersi, H. & Caffrey, M. Crystallization screens: compatibility with the lipidic cubic phase for in meso crystallization of membrane proteins. Biophys J. 81, 225-242 (2001).