Recombinant expression and purification of human TATA binding protein using a chimeric fusion

Protein Expression and Purification

Volumn 85, Issue 1, 2012, Pages 142-147

  Silvers, Robert ^a   Saxena, Krishna ^a   Kudlinzki, Denis ^a   Schwalbe, Harald ^a  

^a GOETHE UNIVERSITY   (Germany)

Author keywords

Fusion protein; MST; NMR; Recombinant expression; TATA binding protein; TBP

Indexed keywords

HYBRID PROTEIN; TATA BINDING PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CHEMICAL STRUCTURE; CHEMISTRY; ESCHERICHIA COLI; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEAR MAGNETIC RESONANCE; PROTEIN FOLDING;

AMINO ACID SEQUENCE; CLONING, MOLECULAR; ESCHERICHIA COLI; HUMANS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN FOLDING; RECOMBINANT FUSION PROTEINS; TATA-BOX BINDING PROTEIN;

EUKARYOTA;

EID: 84864805949     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2012.07.006     Document Type: Article

References (56)

1. R.D. Kornberg The molecular basis of eukaryotic transcription Proc. Natl. Acad. Sci. USA 104 2007 12955 12961 (Pubitemid 351737575)
2. T.I. Lee, and R.A. Young Transcription of eukaryotic protein-coding genes Annu. Rev. Genet. 34 2000 77 137 (Pubitemid 32065917)
3. G. Orphanides, T. Lagrange, and D. Reinberg The general transcription factors of RNA polymerase II Genes Dev. 10 1996 2657 2683 (Pubitemid 26384488)
4. F.T.F. Tsai, and P.B. Sigler Structural basis of preinitiation complex assembly on human Pol II promoters EMBO J. 19 2000 25 36 (Pubitemid 30009222)
5. C. Yang, E. Bolotin, T. Jiang, F.M. Sladek, and E. Martinez Prevalence of the initiator over the TATA box in human and yeast genes and identification of DNA motifs enriched in human TATA-less core promoters Gene 389 2007 52 65 (Pubitemid 46107776)
6. V. McCulloch, P. Hardin, W. Peng, J.M. Ruppert, and S.M. Lobo-Ruppert Alternatively spliced hBRF variants function at different RNA polymerase III promoters EMBO J. 19 2000 4134 4143 (Pubitemid 30608551)
7. Z. Wang, and R.G. Roeder Structure and function of a human transcription factor TFIIIB subunit that is evolutionarily conserved and contains both TFIIB- and high-mobility-group protein 2-related domains Proc. Natl. Acad. Sci. 92 1995 7026 7030
8. P. Wollmann, S. Cui, R. Viswanathan, O. Berninghausen, M.N. Wells, M. Moldt, G. Witte, A. Butryn, P. Wendler, R. Beckmann, D.T. Auble, and K.-P. Hopfner Structure and mechanism of the Swi2/Snf2 remodeller Mot1 in complex with its substrate TBP Nature 475 2011 403 407
9. J.J. Chicca 2nd, D.T. Auble, and B.F. Pugh Cloning and biochemical characterization of TAF-172, a human homolog of yeast Mot1 Mol. Cell. Biol. 18 1998 1701 1710 (Pubitemid 28100936)
10. R. Scully, S.F. Anderson, D.M. Chao, W. Wei, L. Ye, R.A. Young, D.M. Livingston, and J.D. Parvin BRCA1 is a component of the RNA polymerase II holoenzyme Proc. Natl. Acad. Sci. 94 1997 5605 5610 (Pubitemid 27241816)
11. R. Metz, A.J. Bannister, J.A. Sutherland, C. Hagemeier, E.C. O'Rourke, A. Cook, R. Bravo, and T. Kouzarides C-Fos-induced activation of a TATA-box-containing promoter involves direct contact with TATA-box-binding protein Mol. Cell. Biol. 14 1994 6021 6029 (Pubitemid 24264431)
12. C.C. Franklin, A.V. McCulloch, and A.S. Kraft In vitro association between the Jun protein family and the general transcription factors TBP and TFIIB Biochem. J. 305 Pt 3 1995 967 974
13. C. Brendel, L. Gelman, and J. Auwerx Multiprotein bridging factor-1 (MBF-1) is a cofactor for nuclear receptors that regulate lipid metabolism Mol. Endocrinol. 16 2002 1367 1377 (Pubitemid 34621082)
14. Y. Kabe, M. Goto, D. Shima, T. Imai, T. Wada, K.-i. Morohashi, M. Shirakawa, S. Hirose, and H. Handa The Role of Human MBF1 as a transcriptional coactivator J. Biol. Chem. 274 1999 34196 34202 (Pubitemid 129511757)
15. M. Mariotti, L. De Benedictis, E. Avon, and J.A.M. Maier Interaction between endothelial differentiation-related factor-1 and calmodulin in vitro and in vivo J. Biol. Chem. 275 2000 24047 24051 (Pubitemid 30624696)
16. D.A. Bushnell, K.D. Westover, R.E. Davis, R.D. Kornberg, Structural Basis of Transcription: An RNA Polymerase II-TFIIB Cocrystal at 4.5 Angstroms, Science 303 (2004) 983-988. (Pubitemid 38209696)
17. J. DeJong, R. Bernstein, and R.G. Roeder Human general transcription factor TFIIA: characterization of a cDNA encoding the small subunit and requirement for basal and activated transcription Proc. Natl. Acad. Sci. 92 1995 3313 3317
18. M.-F. Langelier, D. Forget, A. Rojas, Y. Porlier, Z.F. Burton, and B. Coulombe Structural and functional interactions of transcription factor (TF) IIA with TFIIE and TFIIF in transcription initiation by RNA polymerase II J. Biol. Chem. 276 2001 38652 38657
19. J. Ozer, K. Mitsouras, D. Zerby, M. Carey, and P.M. Lieberman Transcription factor IIA derepresses TATA-binding protein (TBP)-associated factor inhibition of TBP-DNA binding J. Biol. Chem. 273 1998 14293 14300 (Pubitemid 28319144)
20. S. Solow, M. Salunek, R. Ryan, and P.M. Lieberman TAFII 250 phosphorylates human transcription factor IIA on serine residues important for TBP binding and transcription activity J. Biol. Chem. 276 2001 15886 15892
21. X. Sun, D. Ma, M. Sheldon, K. Yeung, and D. Reinberg Reconstitution of human TFIIA activity from recombinant polypeptides: a role in TFIID-mediated transcription Genes Dev. 8 1994 2336 2348 (Pubitemid 24314295)
22. S. Malik, M. Guermah, and R.G. Roeder A dynamic model for PC4 coactivator function in RNA polymerase II transcription Proc. Natl. Acad. Sci. 95 1998 2192 2197 (Pubitemid 28145917)
23. S. Ruppert, and R. Tjian Human TAFII250 interacts with RAP74: implications for RNA polymerase II initiation Genes Dev. 9 1995 2747 2755 (Pubitemid 3009918)
24. T. Léveillard, and B. Wasylyk The MDM2 C-terminal region binds to TAFII250 and Is required for MDM2 regulation of the cyclin A promoter J. Biol. Chem. 272 1997 30651 30661 (Pubitemid 27527497)
25. C.J. Thut, J.A. Goodrich, and R. Tjian Repression of p53-mediated transcription by MDM2: a dual mechanism Genes Dev. 11 1997 1974 1986 (Pubitemid 27332984)
26. S. Shetty, T. Takahashi, H. Matsui, R. Ayengar, and R. Raghow Transcriptional autorepression of Msx1 gene is mediated by interactions of Msx1 protein with a multi-protein transcriptional complex containing TATA-binding protein, Sp1 and cAMP-response-element-binding protein-binding protein (CBP/p300) Biochem. J. 339 Pt 3 1999 751 758 (Pubitemid 29243541)
27. H. Zhang, K.M. Catron, and C. Abate-Shen A role for the Msx-1 homeodomain in transcriptional regulation: residues in the N-terminal arm mediate TATA binding protein interaction and transcriptional repression Proc. Natl. Acad. Sci. 93 1996 1764 1769 (Pubitemid 26079831)
28. H. Zhang, G. Hu, H. Wang, P. Sciavolino, N. Iler, M.M. Shen, and C. Abate-Shen Heterodimerization of Msx and Dlx homeoproteins results in functional antagonism Mol. Cell. Biol. 17 1997 2920 2932 (Pubitemid 27175176)
29. M. Bellorini, D.K. Lee, J.C. Dantonel, K. Zemzoumi, R.G. Roeder, L. Tora, and R. Mantovani CCAAT binding NF-Y-TBP interactions: NF-YB and NF-YC require short domains adjacent to their histone fold motifs for association with TBP basic residues Nucleic Acids Res. 25 1997 2174 2181 (Pubitemid 27303453)
30. A. Cvekl, F. Kashanchi, J.N. Brady, and J. Piatigorsky Pax-6 interactions with TATA-box-binding protein and retinoblastoma protein Invest. Ophthalmol. Vis. Sci. 40 1999 1343 1350 (Pubitemid 29252324)
31. E. Seto, A. Usheva, G.P. Zambetti, J. Momand, N. Horikoshi, R. Weinmann, A.J. Levine, and T. Shenk Wild-type p53 binds to the TATA-binding protein and represses transcription Proc. Natl. Acad. Sci. 89 1992 12028 12032 (Pubitemid 23004778)
32. S. Zwilling, A. Annweiler, and T. Wirth The POU domains of the Oct1 and Oct2 transcription factors mediate specific interaction with TBP Nucleic Acids Res. 22 1994 1655 1662 (Pubitemid 24155822)
33. M. Guermah, S. Malik, and R.G. Roeder Involvement of TFIID and USA components in transcriptional activation of the human immunodeficiency virus promoter by NF-kappaB and Sp1 Mol. Cell. Biol. 18 1998 3234 3244 (Pubitemid 28240496)
34. M.L. Schmitz, G. Stelzer, H. Altmann, M. Meisterernst, and P.A. Baeuerle Interaction of the COOH-terminal transactivation domain of p65 NF-κB with TATA-binding protein, transcription factor IIB, and coactivators J. Biol. Chem. 270 1995 7219 7226
35. I.G. Schulman, D. Chakravarti, H. Juguilon, A. Romo, and R.M. Evans Interactions between the retinoid X receptor and a conserved region of the TATA-binding protein mediate hormone-dependent transactivation Proc. Natl. Acad. Sci. 92 1995 8288 8292
36. T. O'Brien, and R. Tjian Functional analysis of the human TAFII250 N-terminal kinase domain Mol. Cell 1 1998 905 911 (Pubitemid 128379074)
37. S. Ruppert, E.H. Wang, and R. Tjian Cloning and expression of human TAFII250: a TBP-associated factor implicated in cell-cycle regulation Nature 362 1993 175 179 (Pubitemid 23085260)
38. J.L. Siegert, and P.D. Robbins Rb inhibits the intrinsic kinase activity of TATA-binding protein-associated factor TAFII250 Mol. Cell. Biol. 19 1999 846 854 (Pubitemid 29018485)
39. T.K. Mal, S. Takahata, S. Ki, L. Zheng, T. Kokubo, and M. Ikura Functional silencing of TATA-binding protein (TBP) by a covalent linkage of the N-terminal domain of TBP-associated factor 1 J. Biol. Chem. 282 2007 22228 22238 (Pubitemid 47195744)
40. J.-C. Pointud, G. Mengus, S. Brancorsini, L. Monaco, M. Parvinen, P. Sassone-Corsi, and I. Davidson The intracellular localisation of TAF7L, a paralogue of transcription factor TFIID subunit TAF7, is developmentally regulated during male germ-cell differentiation J. Cell Sci. 116 2003 1847 1858 (Pubitemid 36582171)
41. Y. Tao, M. Guermah, E. Martinez, T. Oelgeschläger, S. Hasegawa, R. Takada, T. Yamamoto, M. Horikoshi, and R.G. Roeder Specific interactions and potential functions of human TAFII100 J. Biol. Chem. 272 1997 6714 6721 (Pubitemid 27118159)
42. E. Martinez, V.B. Palhan, A. Tjernberg, E.S. Lymar, A.M. Gamper, T.K. Kundu, B.T. Chait, and R.G. Roeder Human STAGA complex is a chromatin- acetylating transcription coactivator that interacts with Pre-mRNA splicing and DNA damage-binding factors in vivo Mol. Cell. Biol. 21 2001 6782 6795 (Pubitemid 32911240)
43. M. May, G. Mengus, A.C. Lavigne, P. Chambon, and I. Davidson Human TAF(II28) promotes transcriptional stimulation by activation function 2 of the retinoid X receptors EMBO J. 15 1996 3093 3104
44. G. Mengus, M. May, X. Jacq, A. Staub, L. Tora, P. Chambon, and I. Davidson Cloning and characterization of hTAFII18, hTAFII20 and hTAFII28: three subunits of the human transcription factor TFIID EMBO J. 14 1995 1520 1531
45. A. Hoffmann, and R.G. Roeder Cloning and characterization of human TAF20/15 J. Biol. Chem. 271 1996 18194 18202
46. S.M. Ochs, S. Thumann, R. Richau, M.T. Weirauch, T.M. Lowe, M. Thomm, and W. Hausner Activation of archaeal transcription mediated by recruitment of transcription factor B J. Biol. Chem. 287 2012 18863 18871
47. B. Treutlein, A. Muschielok, J. Andrecka, A. Jawhari, C. Buchen, D. Kostrewa, F. Hög, P. Cramer, and J. Michaelis Dynamic architecture of a minimal RNA polymerase II open promoter complex Mol. Cell 46 2012 136 146
48. D.D. Shooltz, G.L. Alberts, and S.J. Triezenberg One-step affinity purification of recombinant TATA binding proteins utilizing a modular protein interaction partner Protein Expr. Purif. 59 2008 297 301
49. E. Gasteiger, C. Hoogland, A. Gattiker, S.e. Duvaud, M.R. Wilkins, R.D. Appel, A. Bairoch, Protein Identification and Analysis Tools on the ExPASy Server. The Proteomics Protocols Handbook, in: J.M. Walker (Ed.), Humana Press, 2005, pp. 571-607.
50. S.C. Gill, and P.H. von Hippel Calculation of protein extinction coefficients from amino acid sequence data Anal. Biochem. 182 1989 319 326 (Pubitemid 19277112)
51. P. Baaske, C.J. Wienken, P. Reineck, S. Duhr, and D. Braun optical thermophoresis for quantifying the buffer dependence of aptamer binding Angew. Chem. Int. Ed. 49 2010 2238 2241
52. S. Duhr, and D. Braun Why molecules move along a temperature gradient Proc. Natl. Acad. Sci. 103 2006 19678 19682 (Pubitemid 46037491)
53. M. Jerabek-Willemsen, C.J. Wienken, D. Braun, P. Baaske, and S. Duhr Molecular interaction studies using microscale thermophoresis Assay Drug Dev. Technol. 9 2011 342 353
54. C.J. Wienken, P. Baaske, U. Rothbauer, D. Braun, and S. Duhr Protein-binding assays in biological liquids using microscale thermophoresis Nat. Commun. 1 2010 100
55. C.A. Weideman, R.C. Netter, L.R. Benjamin, J.J. McAllister, L.A. Schmiedekamp, R.A. Coleman, and B.F. Pugh Dynamic interplay of TFIIA, TBP and TATA DNA J. Mol. Biol. 271 1997 61 75 (Pubitemid 27338767)
56. A.N. Imbalzano, K.S. Zaret, and R.E. Kingston Transcription factor (TF) IIB and TFIIA can independently increase the affinity of the TATA-binding protein for DNA J. Biol. Chem. 269 1994 8280 8286 (Pubitemid 24200242)