Localization-based super-resolution microscopy with an sCMOS camera Part II: Experimental methodology for comparing sCMOS with EMCCD cameras

Optics Express

Volumn 20, Issue 16, 2012, Pages 17741-17759

  Long, Fan ^a,b   Zeng, Shaoqun ^a,b   Huang, Zhen Li ^a,b  

^a HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CHARGE COUPLED DEVICES; OPTICAL RESOLVING POWER;

COMPLEMENTARY METAL OXIDE SEMICONDUCTORS; ELECTRON MULTIPLYING CHARGE COUPLED DEVICES; EXPERIMENTAL METHODOLOGY; IMAGING PERFORMANCE; LOCALIZATION ACCURACY; SIGNAL RANGE; SINGLE-MOLECULE DETECTION; SUPER-RESOLUTION MICROSCOPY;

CAMERAS;

METAL; OXIDE;

ARTICLE; CHEMISTRY; COMPARATIVE STUDY; ELECTRON; EQUIPMENT; FLUORESCENCE; IMAGE PROCESSING; METHODOLOGY; MICROSCOPY; PHOTOGRAPHY; PHOTON; SEMICONDUCTOR; SIGNAL NOISE RATIO;

ELECTRONS; FLUORESCENCE; IMAGE PROCESSING, COMPUTER-ASSISTED; METALS; MICROSCOPY; OXIDES; PHOTOGRAPHY; PHOTONS; SEMICONDUCTORS; SIGNAL-TO-NOISE RATIO;

EID: 84864579552     PISSN: None     EISSN: 10944087     Source Type: Journal    
DOI: 10.1364/OE.20.017741     Document Type: Article

References (33)

1. B. Huang, H. Babcock, and X. W. Zhuang, "Breaking the diffraction barrier: Super-resolution imaging of cells", Cell 143(7), 1047-1058 (2010).
2. T. J. Gould, V. V. Verkhusha, and S. T. Hess, "Imaging biological structures with fluorescence photoactivation localization microscopy", Nat. Protoc. 4(3), 291-308 (2009).
3. H. Shroff, H. White, and E. Betzig, "Photoactivated localization microscopy (PALM) of adhesion complexes", Curr. Protoc. Cell Biol. Chapter 4, Unit 4.21 (2008).
4. P. Seitz and A. J. P. Theuwissen, Single-photon imaging (Springer, Heidelberg, N. Y., 2011).
5. B. Moomaw, "Camera technologies for low light imaging: overview and relative advantages", Methods Cell Biol. 81, 251-283 (2007).
6. J. W. Lichtman and W. Denk, "The big and the small: Challenges of imaging the brain's circuits", Science 334(6056), 618-623 (2011).
7. M. Bigas, E. Cabruja, J. Forest, and J. Salvi, "Review of CMOS image sensors", Microelectron. J. 37(5), 433-451 (2006). (Pubitemid 43344043)
8. B. Fowler, C. A. Liu, S. Mims, J. Balicki, W. Li, H. Do, J. Appelbaum, and P. Vu, "A 5.5Mpixel 100 frames/sec wide dynamic range low noise CMOS image sensor for scientific applications", Proc. SPIE 7536, 753607, 753607-12 (2010).
9. J. R. Joubert and D. K. Sharma, "EMCCD vs. sCMOS for microscopic imaging", Photon. Spectra 45, 46-50 (2011).
10. G. Holst, "Scientific CMOS image sensors", Laser Photon. 5, 18-21 (2009).
11. http://sales.hamamatsu.com/assets/pdf/hpspdf/Flash4-ChangingTheGame.pdf, accessed May 2012.
12. http://www.scmos.com/files/high/scmos-white-paper-8mb.pdf, accessed May 2012.
13. Z. L. Huang, H. Y. Zhu, F. Long, H. Q. Ma, L. S. Qin, Y. F. Liu, J. P. Ding, Z. H. Zhang, Q. M. Luo, and S. Q. Zeng, "Localization-based super-resolution microscopy with an sCMOS camera", Opt. Express 19(20), 19156-19168 (2011).
14. S. Saurabh, S. Maji, and M. P. Bruchez, "Evaluation of sCMOS cameras for detection and localization of single Cy5 molecules", Opt. Express 20(7), 7338-7349 (2012).
15. J. R. Janesick, Photon transfer: DN [lambda] (SPIE, Bellingham, Wash., 2007).
16. A. El Gamal, B. Fowlera, H. Min, and X. Q. Liu, "Modeling and estimation of FPN components in CMOS image", Proc. SPIE 3301, 168-177 (1998). (Pubitemid 35004112)
17. J. B. Pawley, Handbook of Biological Confocal Microscopy, 3rd ed. (Springer, 2006).
18. T. W. Quan, S. Q. Zeng, and Z. L. Huang, "Localization capability and limitation of electron-multiplying charge-coupled, scientific complementary metal-oxide semiconductor, and charge-coupled devices for superresolution imaging", J. Biomed. Opt. 15(6), 066005 (2010).
19. R. J. Ober, S. Ram, and E. S. Ward, "Localization accuracy in single-molecule microscopy", Biophys. J. 86(2), 1185-1200 (2004). (Pubitemid 38213119)
20. R. E. Thompson, D. R. Larson, and W. W. Webb, "Precise nanometer localization analysis for individual fluorescent probes", Biophys. J. 82(5), 2775-2783 (2002). (Pubitemid 34441314)
21. M. A. Thompson, J. S. Biteen, S. J. Lord, N. R. Conley, and W. E. Moerner, "Molecules and methods for super-resolution imaging", Methods Enzymol. 475, 27-59 (2010).
22. S. A. Jones, S. H. Shim, J. He, and X. Zhuang, "Fast, three-dimensional super-resolution imaging of live cells", Nat. Methods 8(6), 499-505 (2011).
23. T. W. Quan, P. C. Li, F. Long, S. Q. Zeng, Q. M. Luo, P. N. Hedde, G. U. Nienhaus, and Z. L. Huang, "Ultra-fast, high-precision image analysis for localization-based super resolution microscopy", Opt. Express 18(11), 11867-11876 (2010).
24. http://www.andor.com/pdfs/spec-sheets/L897SS.pdf, accessed May 2012.
25. http://www.photometrics.com/products/datasheets/HQ2.pdf, accessed May 2012.
26. http://sales.hamamatsu.com/assets/pdf/hpspdf/e-orcaFlashE01.pdf, accessed May 2012.
27. http://sales.hamamatsu.com/assets/pdf/hpspdf/e-flash4.pdf, accessed May 2012.
28. K. I. Mortensen, L. S. Churchman, J. A. Spudich, and H. Flyvbjerg, "Optimized localization analysis for single-molecule tracking and super-resolution microscopy", Nat. Methods 7(5), 377-381 (2010).
29. M. C. DeSantis, S. H. DeCenzo, J. L. Li, and Y. M. Wang, "Precision analysis for standard deviation measurements of immobile single fluorescent molecule images", Opt. Express 18(7), 6563-6576 (2010).
30. M. Fernández-Suárez and A. Y. Ting, "Fluorescent probes for super-resolution imaging in living cells", Nat. Rev. Mol. Cell Biol. 9(12), 929-943 (2008).
31. G. T. Dempsey, J. C. Vaughan, K. H. Chen, M. Bates, and X. W. Zhuang, "Evaluation of fluorophores for optimal performance in localization-based super-resolution imaging", Nat. Methods 8(12), 1027-1036 (2011).
32. M. F. Snoeij, A. J. P. Theuwissen, K. A. A. Makinwa, and J. H. Huijsing, "A CMOS imager with column-level ADC using dynamic column fixed-pattern noise reduction", IEEE J. Solid-st. Circulation 41, 3007-3015 (2006). (Pubitemid 44955526)
33. M. S. Robbins and B. J. Hadwen, "The noise performance of electron multiplying charge-coupled devices", IEEE Trans. Electron. Dev. 50(5), 1227-1232 (2003).