Validation of the β-amy1 transcription profiling assay and selection of reference genes suited for a RT-qPCR assay in developing barley caryopsis

PLoS ONE

Volumn 7, Issue 7, 2012, Pages

  Ovesná, Jaroslava ^a   Kučera, Ladislav ^a   Vaculová, Kateřina ^a   Štrymplová, Kamila ^a   Svobodová, Ilona ^b   Milella, Luigi ^c  

^a CROP RESEARCH INSTITUTE   (Czech Republic)

^b Agrotests phyto Ltd ^*  (Czech Republic)

^c UNIVERSITY OF BASILICATA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE DIPHOSPHATE RIBOSYLATION FACTOR; BETA AMYLASE; COMPLEMENTARY DNA; ELONGATION FACTOR; ELONGATION FACTOR 1ALPHA; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; HEAT SHOCK PROTEIN 70; HEAT SHOCK PROTEIN 90; S ADENOSYLMETHIONINE; UBIQUITIN;

AMINO ACID SEQUENCE; ARTICLE; BARLEY; BETA AMY1 GENE; CONTROLLED STUDY; DNA SYNTHESIS; ENZYME ACTIVITY; GENE EXPRESSION; NONHUMAN; PLANT GENE; PROTEIN STABILITY; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION;

BETA-AMYLASE; GENE EXPRESSION; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, PLANT; GENES, PLANT; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; HORDEUM; PLANT PROTEINS; REAL-TIME POLYMERASE CHAIN REACTION; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS; SEEDS;

CARYOPSIS; HORDEUM;

EID: 84864492310     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0041886     Document Type: Article

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