Structural characteristics determine the cause of the low enzyme activity of two thiopurine S-methyltransferase allelic variants: A biophysical characterization of TPMT*2 and TPMT*5

Biochemistry

Volumn 51, Issue 30, 2012, Pages 5912-5920

  Wennerstrand, Patricia ^a   Dametto, Paolo ^a   Hennig, Janosch ^a   Klingstedt, Therése ^a   Skoglund, Karin ^a   Lindqvist Appell, Malin ^a   Mårtensson, Lars Göran ^a  

^a LINKÖPING UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE; ACUTE LYMPHOBLASTIC LEUKEMIA; ALLELIC VARIANTS; BIOPHYSICAL CHARACTERIZATION; C-TERMINUS; CLEAVAGE PATTERNS; FIBRIL FORMATION; FLUORESCENT MOLECULES; IN-VITRO; IN-VIVO; INFLAMMATORY BOWEL DISEASE; LIMITED PROTEOLYSIS; MOLECULAR LEVELS; NATURALLY OCCURRING; OLIGOTHIOPHENES; STABLE REGION; STRUCTURAL CHANGE; STRUCTURAL CHARACTERISTICS; TERTIARY STRUCTURES; THERMAL DENATURATIONS; WILD TYPES;

AMINO ACIDS; CIRCULAR DICHROISM SPECTROSCOPY; CLONING; DEGRADATION; ESCHERICHIA COLI; GENES;

PROBES;

8 ANILINO 1 NAPHTHALENESULFONIC ACID; AMINO ACID; THIOPURINE METHYLTRANSFERASE; THIOPURINE METHYLTRANSFERASE 2; THIOPURINE METHYLTRANSFERASE 5; UNCLASSIFIED DRUG;

ALLELE; ARTICLE; CIRCULAR DICHROISM; DENATURATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME CONFORMATION; ESCHERICHIA COLI; LIGHT SCATTERING; MOLECULAR CLONING; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN STABILITY; THERMAL DENATURATION;

ALLELES; AMINO ACID SEQUENCE; CATALYTIC DOMAIN; ENZYME ACTIVATION; GENETIC VARIATION; HUMANS; METHYLTRANSFERASES; MOLECULAR SEQUENCE DATA;

ESCHERICHIA COLI;

EID: 84864483207     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi300377d     Document Type: Article

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