Mammalian fatty acid synthase activity from crude tissue lysates tracing 13C-labeled substrates using gas chromatography-mass spectrometry

Analytical Biochemistry

Volumn 428, Issue 2, 2012, Pages 158-166

  Rudolph, Michael C ^a   Karl Maluf, N ^b   Wellberg, Elizabeth A ^a   Johnson, Chris A ^a   Murphy, Robert C ^a   Anderson, Steve M ^a  

^a UNIVERSITY OF COLORADO SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF COLORADO   (United States)

Author keywords

Fatty acid synthase; Gas chromatography mass spectrometry; Mammary gland liver lysates

Indexed keywords

BIOSYNTHESIS; CARBOXYLATION; ENZYMES; MAMMALS; MASS SPECTROMETRY; PALMITIC ACID; PHYSIOLOGICAL MODELS; TISSUE;

FATTY ACID SYNTHASE; FEMTOMOLE QUANTITIES; GAS CHROMATOGRAPHY-MASS SPECTROMETRIES (GC-MS); GAS CHROMATOGRAPHY-MASS SPECTROMETRY; LYSATES; NICOTINAMIDE ADENINE DINUCLEOTIDES; PHYSIOLOGICAL STATE; STEADY-STATE KINETICS;

GAS CHROMATOGRAPHY;

ACETYL COENZYME A; CARBON 13; FATTY ACID SYNTHASE; MALONYL COENZYME A; PALMITIC ACID; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ANIMAL TISSUE; ARTICLE; BIOASSAY; CELL LYSATE; ENZYME ACTIVITY; ENZYME KINETICS; LIVER; MAMMARY GLAND; MASS FRAGMENTOGRAPHY; MOUSE; NONHUMAN; PRIORITY JOURNAL;

MAMMALIA;

EID: 84864402386     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.06.013     Document Type: Article

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