A single-step purification and molecular characterization of functional Shiga toxin 2 variants from pathogenic Escherichia coli

Toxins

Volumn 4, Issue 7, 2012, Pages 487-504

  He, Xiaohua ^a   Quiñones, Beatriz ^a   McMahon, Stephanie ^a   Mandrell, Robert E ^a  

^a WESTERN REGIONAL RESEARCH CENTER   (United States)

Author keywords

Cell free translation assay; Cytotoxicity; Purification of Shiga toxins; Shiga toxin 2 variants; Thermal stability of Shiga toxins

Indexed keywords

MONOCLONAL ANTIBODY; SHIGA TOXIN; UNCLASSIFIED DRUG; VEROTOXIN 2; VEROTOXIN 2A; VEROTOXIN 2C; VEROTOXIN 2D; VEROTOXIN 2G;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; BACTERIAL VIRULENCE; BACTERIUM CULTURE; CELL FREE SYSTEM; CHEMICAL STRUCTURE; CYTOTOXICITY; DENATURING GRADIENT GEL ELECTROPHORESIS; HELA CELL; HUMAN; HUMAN CELL; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROTEIN DENATURATION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS INHIBITION; RABBIT; RETICULOCYTE LYSATE; SEQUENCE ANALYSIS; SHIGA TOXIN PRODUCING ESCHERICHIA COLI; SUPERNATANT; TEMPERATURE; THERMOSTABILITY; TOXIN ANALYSIS; WESTERN BLOTTING;

ANIMALS; BLOTTING, WESTERN; CELL-FREE SYSTEM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HELA CELLS; HUMANS; RABBITS; RETICULOCYTES; SHIGA TOXIN 2;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; ORYCTOLAGUS CUNICULUS;

EID: 84864372320     PISSN: 20726651     EISSN: None     Source Type: Journal    
DOI: 10.3390/toxins4070487     Document Type: Article

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