메뉴 건너뛰기




Volumn 77, Issue 11, 2011, Pages 3558-3564

Sensitive detection of Shiga toxin 2 and some of Its variants in environmental samples by a novel immuno-PCR assay

Author keywords

[No Author keywords available]

Indexed keywords

CULTURE METHODS; DETECTION LIMITS; DETECTION THRESHOLD; DNA MARKERS; ENVIRONMENTAL SAMPLE; ENZYME LINKED IMMUNOSORBENT ASSAY; PHOSPHATE-BUFFERED SALINES; REAL-TIME PCR; ROBUST DETECTION; SCREENING METHODS; SENSITIVE DETECTION; SENSITIVITY AND SPECIFICITY; SHIGA TOXIN;

EID: 79958294582     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02205-10     Document Type: Article
Times cited : (36)

References (50)
  • 1
    • 3042807048 scopus 로고    scopus 로고
    • Lowering the detection limits of HIV-1 viral load using real-time immuno-PCR for HIV-1 p24 antigen
    • Barletta, J. M., D. C. Edelman, and N. T. Constantine. 2004. Lowering the detection limits of HIV-1 viral load using real-time immuno-PCR for HIV-1 p24 antigen. Am. J. Clin. Pathol. 122, 20-27.
    • (2004) Am. J. Clin. Pathol. , vol.122 , pp. 20-27
    • Barletta, J.M.1    Edelman, D.C.2    Constantine, N.T.3
  • 2
    • 34247340157 scopus 로고    scopus 로고
    • The non-O157 Shiga-toxigenic (verocytotoxigenic) Escherichia coli; under-rated pathogens
    • Bettelheim, K. A. 2007. The non-O157 Shiga-toxigenic (verocytotoxigenic) Escherichia coli; under-rated pathogens. Crit. Rev. Microbiol. 33, 67-87.
    • (2007) Crit. Rev. Microbiol. , vol.33 , pp. 67-87
    • Bettelheim, K.A.1
  • 3
    • 0042661253 scopus 로고    scopus 로고
    • Rapid laboratory identification and characterization of verocytotoxigenic (Shiga toxin producing) Escherichia coli (VTEC/STEC)
    • Bettelheim, K. A., and L. Beutin. 2003. Rapid laboratory identification and characterization of verocytotoxigenic (Shiga toxin producing) Escherichia coli (VTEC/STEC). J. Appl. Microbiol. 95, 205-217.
    • (2003) J. Appl. Microbiol. , vol.95 , pp. 205-217
    • Bettelheim, K.A.1    Beutin, L.2
  • 4
    • 0033024964 scopus 로고    scopus 로고
    • Associations between virulence factors of Shiga toxin-producing Escherichia coli and disease in humans
    • Boerlin, P., et al. 1999. Associations between virulence factors of Shiga toxin-producing Escherichia coli and disease in humans. J. Clin. Microbiol. 37, 497-503.
    • (1999) J. Clin. Microbiol. , vol.37 , pp. 497-503
    • Boerlin, P.1
  • 5
    • 25844432970 scopus 로고    scopus 로고
    • Non- O157 Shiga toxin-producing Escherichia coli infections in the United States 1983-2002
    • Brooks, J. T., et al. 2005. Non-O157 Shiga toxin-producing Escherichia coli infections in the United States, 1983-2002. J. Infect. Dis. 192, 1422-1429.
    • (2005) J. Infect. Dis. , vol.192 , pp. 1422-1429
    • Brooks, J.T.1
  • 6
    • 1242314782 scopus 로고    scopus 로고
    • A highly sensitive immuno-polymerase chain reaction assay for Clostridium botulinum neurotoxin type A
    • Chao, H. Y., Y. C. Wang, S. S. Tang, and H. W. Liu. 2004. A highly sensitive immuno-polymerase chain reaction assay for Clostridium botulinum neurotoxin type A. Toxicon 43, 27-34.
    • (2004) Toxicon , vol.43 , pp. 27-34
    • Chao, H.Y.1    Wang, Y.C.2    Tang, S.S.3    Liu, H.W.4
  • 7
    • 37049010237 scopus 로고    scopus 로고
    • Incidence and tracking of Escherichia coli O157 H7 in a major produce production region in California
    • Cooley, M., et al. 2007. Incidence and tracking of Escherichia coli O157, H7 in a major produce production region in California. PLoS One 2, e1159.
    • (2007) PLoS One , vol.2
    • Cooley, M.1
  • 8
    • 0024375644 scopus 로고
    • Globotetraosylceramide is recognized by the pig edema disease toxin
    • DeGrandis, S., H. Law, J. Brunton, C. Gyles, and C. A. Lingwood. 1989. Globotetraosylceramide is recognized by the pig edema disease toxin. J. Biol. Chem. 264, 12520-12525.
    • (1989) J. Biol. Chem. , vol.264 , pp. 12520-12525
    • DeGrandis, S.1    Law, H.2    Brunton, J.3    Gyles, C.4    Lingwood, C.A.5
  • 9
    • 0025731541 scopus 로고
    • Waterborne outbreak of Escherichia coli O157
    • Dev, V. J., M. Main, and I. Gould. 1991. Waterborne outbreak of Escherichia coli O157. Lancet 337, 1412.
    • (1991) Lancet , vol.337 , pp. 1412
    • Dev, V.J.1    Main, M.2    Gould, I.3
  • 10
    • 0023947126 scopus 로고
    • The RNA N-glycosidase activity of ricin A-chain The characteristics of the enzymatic activity of ricin A-chain with ribosomes and with rRNA
    • Endo, Y., and K. Tsurugi. 1988. The RNA N-glycosidase activity of ricin A-chain. The characteristics of the enzymatic activity of ricin A-chain with ribosomes and with rRNA. J. Biol. Chem. 263, 8735-8739.
    • (1988) J. Biol. Chem. , vol.263 , pp. 8735-8739
    • Endo, Y.1    Tsurugi, K.2
  • 11
    • 0141643388 scopus 로고    scopus 로고
    • Comparison of different PCR tests for detecting Shiga toxin-producing Escherichia coli O157 and development of an ELISA-PCR assay for specific identification of the bacteria
    • Fach, P., S. Perelle, J. Grout, and F. Dilasser. 2003. Comparison of different PCR tests for detecting Shiga toxin-producing Escherichia coli O157 and development of an ELISA-PCR assay for specific identification of the bacteria. J. Microbiol. Methods 55, 383-92.
    • (2003) J. Microbiol. Methods , vol.55 , pp. 383-392
    • Fach, P.1    Perelle, S.2    Grout, J.3    Dilasser, F.4
  • 12
    • 34247849222 scopus 로고    scopus 로고
    • A quantitative real-time immuno-PCR approach for detection of staphylococcal enterotoxins
    • Fischer, A., et al. 2007. A quantitative real-time immuno-PCR approach for detection of staphylococcal enterotoxins. J. Mol. Med. 5, 461-469.
    • (2007) J. Mol. Med. , vol.85 , pp. 461-469
    • Fischer, A.1
  • 13
    • 0028367338 scopus 로고
    • Crystal structure of the holotoxin from Shigella dysenteriae at 2 5 A resolution
    • Fraser, M. E., M. M. Chernaia, Y. V. Kozlov, and M. N. James. 1994. Crystal structure of the holotoxin from Shigella dysenteriae at 2.5 A resolution. Nat. Struct. Biol. 1, 59-64.
    • (1994) Nat. Struct. Biol. , vol.1 , pp. 59-64
    • Fraser, M.E.1    Chernaia, M.M.2    Kozlov, Y.V.3    James, M.N.4
  • 14
    • 3042544923 scopus 로고    scopus 로고
    • Structure of Shiga toxin type 2 (Stx2) from Escherichia coli O157
    • Fraser, M. E., et al. 2004. Structure of Shiga toxin type 2 (Stx2) from Escherichia coli O157, H7. J. Biol. Chem. 279, 27511-7517.
    • (2004) H7. J. Biol. Chem. , vol.279 , pp. 27511-27517
    • Fraser, M.E.1
  • 15
    • 79955876027 scopus 로고    scopus 로고
    • Detection by multiplex real-time polymerase chain reaction assays and isolation of Shiga toxin-producing
    • doi: 10.1089/fpd.2010.0773, serogroups O26, O45, O103, O111, O121, and O145 in ground beef. Foodborne Pathog. Dis. [Epub ahead of print.]
    • Fratamico, P. M., et al. 2011. Detection by multiplex real-time polymerase chain reaction assays and isolation of Shiga toxin-producing Escherichia coli serogroups O26, O45, O103, O111, O121, and O145 in ground beef. Foodborne Pathog. Dis. [Epub ahead of print.] doi: 10.1089/fpd.2010.0773.
    • (2011) Escherichia coli
    • Fratamico, P.M.1
  • 16
    • 51849141290 scopus 로고    scopus 로고
    • Longterm survival of Shiga toxin-producing Escherichia coli in cattle effluents and environment, an updated review
    • Fremaux, B., C. Prigent-Combaret, and C. Vernozy-Rozand. 2008. Longterm survival of Shiga toxin-producing Escherichia coli in cattle effluents and environment, an updated review. Vet. Microbiol. 132, 1-18.
    • (2008) Vet. Microbiol. , vol.132 , pp. 1-18
    • Fremaux, B.1    Prigent-Combaret, C.2    Vernozy-Rozand, C.3
  • 17
    • 67650079045 scopus 로고    scopus 로고
    • Rapid and sensitive detection of Shiga toxin-producing Escherichia coli from nonenriched stool specimens by real-time PCR in comparison to enzyme immunoassay and culture
    • Grys, T. E., L. M. Sloan, J. E. Rosenblatt, and R. Patel. 2009. Rapid and sensitive detection of Shiga toxin-producing Escherichia coli from nonenriched stool specimens by real-time PCR in comparison to enzyme immunoassay and culture. J. Clin. Microbiol. 7, 2008-2012.
    • (2009) J. Clin. Microbiol. , vol.47 , pp. 2008-2012
    • Grys, T.E.1    Sloan, L.M.2    Rosenblatt, J.E.3    Patel, R.4
  • 18
    • 34248680659 scopus 로고    scopus 로고
    • Shiga toxin-producing Escherichia coli, an overview
    • Gyles, C. L. 2007. Shiga toxin-producing Escherichia coli, an overview. J. Anim. Sci. 85, E45-E62.
    • (2007) J. Anim. Sci. , vol.85
    • Gyles, C.L.1
  • 20
    • 77951199409 scopus 로고    scopus 로고
    • Development of a novel immuno-PCR assay for detection of ricin in ground beef, liquid chicken egg, and milk
    • He, X., S. McMahon, T. A. McKeon, and D. L. Brandon. 2010. Development of a novel immuno-PCR assay for detection of ricin in ground beef, liquid chicken egg, and milk. J. Food Prot. 73, 695-700.
    • (2010) J. Food Prot. , vol.73 , pp. 695-700
    • He, X.1    McMahon, S.2    McKeon, T.A.3    Brandon, D.L.4
  • 21
    • 67149113525 scopus 로고    scopus 로고
    • Validation of a cell-free translation assay for detecting Shiga toxin 2 in bacterial culture
    • He, X., B. Quinones, J. M. Carter, and R. E. Mandrell. 2009. Validation of a cell-free translation assay for detecting Shiga toxin 2 in bacterial culture. J. Agric. Food Chem. 57, 5084-5088.
    • (2009) J. Agric. Food Chem. , vol.57 , pp. 5084-5088
    • He, X.1    Quinones, B.2    Carter, J.M.3    Mandrell, R.E.4
  • 22
    • 26644464846 scopus 로고    scopus 로고
    • Prevalence of Shiga toxin-producing Escherichia coli in beef cattle
    • Hussein, H. S., and L. M. Bollinger. 2005. Prevalence of Shiga toxin-producing Escherichia coli in beef cattle. J. Food Prot. 68, 224-2241.
    • (2005) J. Food Prot. , vol.68 , pp. 2224-2241
    • Hussein, H.S.1    Bollinger, L.M.2
  • 23
    • 0036794792 scopus 로고    scopus 로고
    • Multiplex fluorogenic real-time PCR for detection and quantification of Escherichia coli O157 H7 in dairy wastewater wetlands
    • Ibekwe, A. M., P. M. Watt, C. M. Grieve, V. K. Sharma, and S. R. Lyons. 2002. Multiplex fluorogenic real-time PCR for detection and quantification of Escherichia coli O157, H7 in dairy wastewater wetlands. Appl. Environ. Microbiol. 68, 4853-4862.
    • (2002) Appl. Environ. Microbiol. , vol.68 , pp. 4853-4862
    • Ibekwe, A.M.1    Watt, P.M.2    Grieve, C.M.3    Sharma, V.K.4    Lyons, S.R.5
  • 24
    • 1542645915 scopus 로고    scopus 로고
    • Subtyping of virulence genes in verocytotoxinproducing Escherichia coli (VTEC) other than serogroup O157 associated with disease in the United Kingdom
    • Jenkins, C., et al. 2003. Subtyping of virulence genes in verocytotoxinproducing Escherichia coli (VTEC) other than serogroup O157 associated with disease in the United Kingdom. J. Med. Microbiol. 52, 941-947.
    • (2003) J. Med. Microbiol. , vol.52 , pp. 941-947
    • Jenkins, C.1
  • 25
    • 0026681538 scopus 로고
    • Frequent loss of Shiga-like toxin genes in clinical isolates of Escherichia coli upon subcultivation
    • Karch, H., T. Meyer, H. Russmann, and J. Heesemann. 1992. Frequent loss of Shiga-like toxin genes in clinical isolates of Escherichia coli upon subcultivation. Infect. Immun. 60, 3464-3467.
    • (1992) Infect. Immun. , vol.60 , pp. 3464-3467
    • Karch, H.1    Meyer, T.2    Russmann, H.3    Heesemann, J.4
  • 26
    • 24044469996 scopus 로고    scopus 로고
    • Enterohaemorrhagic Escherichia coli in human medicine
    • Karch, H., P. I. Tarr, and M. Bielaszewska. 2005. Enterohaemorrhagic Escherichia coli in human medicine. Int. J. Med. icrobiol. 295, 405-418.
    • (2005) Int. J. Med. Microbiol. , vol.295 , pp. 405-418
    • Karch, H.1    Tarr, P.I.2    Bielaszewska, M.3
  • 27
    • 0034042038 scopus 로고    scopus 로고
    • Restriction-site-specific PCR as a rapid test to detect enterohemorrhagic Escherichia coli O157 H7 strains in environmental samples
    • Kimura, R., R. E. Mandrell, J. C. Galland, D. Hyatt, and L. W. Riley. 2000. Restriction-site-specific PCR as a rapid test to detect enterohemorrhagic Escherichia coli O157, H7 strains in environmental samples. Appl. Environ. Microbiol. 66, 2513-2519.
    • (2000) Appl. Environ. Microbiol. , vol.66 , pp. 2513-2519
    • Kimura, R.1    Mandrell, R.E.2    Galland, J.C.3    Hyatt, D.4    Riley, L.W.5
  • 28
    • 0027488402 scopus 로고
    • Verotoxins and their glycolipid receptors
    • Lingwood, C. A. 1993. Verotoxins and their glycolipid receptors. Adv. Lipid Res. 25, 189-211.
    • (1993) Adv. Lipid Res. , vol.25 , pp. 189-211
    • Lingwood, C.A.1
  • 29
    • 33846672995 scopus 로고    scopus 로고
    • Surveillance for Shiga toxin-producing Escherichia coli, Michigan
    • Manning, S. D., et al. 2007. Surveillance for Shiga toxin-producing Escherichia coli, Michigan, 2001-2005. Emerg. Infect. Dis. 3, 318-321.
    • (2007) 2001-2005. Emerg. Infect. Dis. , vol.13 , pp. 318-321
    • Manning, S.D.1
  • 30
    • 48749112422 scopus 로고    scopus 로고
    • Analysis of collection of hemolytic uremic syndrome-associated enterohemorrhagic Escherichia coli
    • Mellmann, A., et al. 2008. Analysis of collection of hemolytic uremic syndrome-associated enterohemorrhagic Escherichia coli. Emerg. Infect. Dis. 14, 1287-1290.
    • (2008) Emerg. Infect. Dis. , vol.14 , pp. 1287-1290
    • Mellmann, A.1
  • 31
    • 0030035066 scopus 로고    scopus 로고
    • Regulation of the Shiga-like toxin II operon in Escherichia coli
    • Muhldorfer, I., et al. 1996. Regulation of the Shiga-like toxin II operon in Escherichia coli. Infect. Immun. 64, 495-502.
    • (1996) Infect. Immun. , vol.64 , pp. 495-502
    • Muhldorfer, I.1
  • 32
    • 33845305037 scopus 로고    scopus 로고
    • Occurrence of Escherichia coli O157 H7 and other enterohemorrhagic Escherichia coli in the environment
    • Muniesa, M., J. Jofre, C. Garcia-Aljaro, and A. R. Blanch. 2006. Occurrence of Escherichia coli O157, H7 and other enterohemorrhagic Escherichia coli in the environment. Environ. Sci. Technol. 40, 7141-7149.
    • (2006) Environ. Sci. Technol. , vol.40 , pp. 7141-7149
    • Muniesa, M.1    Jofre, J.2    Garcia-Aljaro, C.3    Blanch, A.R.4
  • 33
    • 62449181018 scopus 로고    scopus 로고
    • Shiga toxins, glycosphingolipid diversity, and endothelial cell injury
    • Müthing, J., C. H. Schweppe, H. Karch, and A. W. Friedrich. 2009. Shiga toxins, glycosphingolipid diversity, and endothelial cell injury. Thromb. Haemost. 101, 252-264.
    • (2009) Thromb. Haemost. , vol.101 , pp. 252-264
    • Müthing, J.1    Schweppe, C.H.2    Karch, H.3    Friedrich, A.W.4
  • 34
    • 0033861682 scopus 로고    scopus 로고
    • Escherichia coli Shiga toxin
    • Nakao, H., and T. Takeda. 2000. Escherichia coli Shiga toxin. J. Nat. Toxins 9, 299-313.
    • (2000) J. Nat. Toxins , vol.9 , pp. 299-313
    • Nakao, H.1    Takeda, T.2
  • 35
    • 34548154323 scopus 로고    scopus 로고
    • Detecting antigens by quantitative immuno-PCR
    • Niemeyer, C. M., M. Adler, and R. Wacker. 2007. Detecting antigens by quantitative immuno-PCR. Nat. Protoc. 2, 1918-1930.
    • (2007) Nat. Protoc. , vol.2 , pp. 1918-1930
    • Niemeyer, C.M.1    Adler, M.2    Wacker, R.3
  • 36
    • 77955878047 scopus 로고    scopus 로고
    • Foodborne illness, is it on the rise?
    • Nyachuba, D. G. 2010. Foodborne illness, is it on the rise? Nutr. Rev. 68, 257-269.
    • (2010) Nutr. Rev. , vol.68 , pp. 257-269
    • Nyachuba, D.G.1
  • 37
    • 0026704042 scopus 로고
    • Shiga toxin, biochemistry, genetics, mode of action, and role in pathogenesis
    • O'Brien, A. D., et al. 1992. Shiga toxin, biochemistry, genetics, mode of action, and role in pathogenesis. Curr. Top. Microbiol. Immunol. 180, 65-94.
    • (1992) Curr. Top. Microbiol. Immunol. , vol.180 , pp. 65-94
    • O'Brien, A.D.1
  • 38
    • 0020662906 scopus 로고
    • Escherichia coli O157, H7 strains associated with haemorrhagic colitis in the United States produce a
    • (Shiga) like cytotoxin. Lancet i
    • O'Brien, A. O., T. A. Lively, M. E. Chen, S. W. Rothman, and S. B. Formal. 1983. Escherichia coli O157, H7 strains associated with haemorrhagic colitis in the United States produce a Shigella dysenteriae 1 (Shiga) like cytotoxin. Lancet i, 702.
    • (1983) Shigella dysenteriae , vol.1 , pp. 702
    • O'Brien, A.O.1    Lively, T.A.2    Chen, M.E.3    Rothman, S.W.4    Formal, S.B.5
  • 39
    • 35548955176 scopus 로고    scopus 로고
    • The Shiga toxin genotype rather than the amount of Shiga toxin or the cytotoxicity of Shiga toxin in vitro correlates with the appearance of the hemolytic uremic syndrome
    • Orth, D., et al. 2007. The Shiga toxin genotype rather than the amount of Shiga toxin or the cytotoxicity of Shiga toxin in vitro correlates with the appearance of the hemolytic uremic syndrome. Diagn. Microbiol. Infect. Dis. 59, 235-242.
    • (2007) Diagn. Microbiol. Infect. Dis. , vol.59 , pp. 235-242
    • Orth, D.1
  • 40
    • 0024369158 scopus 로고
    • Toxin genotypes and plasmid profiles as determinants of systemic sequelae in Escherichia coli O157 H7 infections
    • Ostroff, S. M., et al. 1989. Toxin genotypes and plasmid profiles as determinants of systemic sequelae in Escherichia coli O157, H7 infections. J. Infect. Dis. 160, 994-998.
    • (1989) J. Infect. Dis. , vol.160 , pp. 994-998
    • Ostroff, S.M.1
  • 41
    • 0031671439 scopus 로고    scopus 로고
    • Identification of new verocytotoxin type 2 variant B-subunit genes in human and animal Escherichia coli isolates
    • Pierard, D., G. Muyldermans, L. Moriau, D. Stevens, and S. Lauwers. 1998. Identification of new verocytotoxin type 2 variant B-subunit genes in human and animal Escherichia coli isolates. J. Clin. Microbiol. 36, 3317-3322.
    • (1998) J. Clin. Microbiol. , vol.36 , pp. 3317-3322
    • Pierard, D.1    Muyldermans, G.2    Moriau, L.3    Stevens, D.4    Lauwers, S.5
  • 42
  • 43
    • 0029034386 scopus 로고
    • A rapid, direct method for enumerating respiring enterohemorrhagic Escherichia coli O157 H7 in water
    • Pyle, B. H., S. C. Broadaway, and G. A. McFeters. 1995. A rapid, direct method for enumerating respiring enterohemorrhagic Escherichia coli O157, H7 in water. Appl. Environ. Microbiol. 61, 2614-2619.
    • (1995) Appl. Environ. Microbiol. , vol.61 , pp. 2614-2619
    • Pyle, B.H.1    Broadaway, S.C.2    McFeters, G.A.3
  • 44
    • 61649106757 scopus 로고    scopus 로고
    • Novel cell-based method to detect Shiga toxin 2 from Escherichia coli O157 H7 and inhibitors of toxin activity
    • Quiñones, B., S. Massey, M. Friedman, M. S. Swimley, and K. Teter. 2009. Novel cell-based method to detect Shiga toxin 2 from Escherichia coli O157, H7 and inhibitors of toxin activity. Appl. Environ. Microbiol. 75, 1410- 1416.
    • (2009) Appl. Environ. Microbiol. , vol.75 , pp. 1410-1416
    • Quiñones, B.1    Massey, S.2    Friedman, M.3    Swimley, M.S.4    Teter, K.5
  • 45
    • 0034008946 scopus 로고    scopus 로고
    • A new Shiga toxin 2 variant (Stx2f) from Escherichia coli isolated from pigeons
    • Schmidt, H., et al. 2000. A new Shiga toxin 2 variant (Stx2f) from Escherichia coli isolated from pigeons. Appl. Environ. Microbiol. 66, 1205-1208.
    • (2000) Appl. Environ. Microbiol. , vol.66 , pp. 1205-1208
    • Schmidt, H.1
  • 46
    • 0035406381 scopus 로고    scopus 로고
    • Quantitative detection of Escherichia coli O157 in surface waters by using immunomagnetic electrochemiluminescence
    • Shelton, D. R., and J. S. Karns. 2001. Quantitative detection of Escherichia coli O157 in surface waters by using immunomagnetic electrochemiluminescence. Appl. Environ. Microbiol. 67, 2908-2915.
    • (2001) Appl. Environ. Microbiol. , vol.67 , pp. 2908-2915
    • Shelton, D.R.1    Karns, J.S.2
  • 48
    • 0035101012 scopus 로고    scopus 로고
    • Role for a phage promoter in Shiga toxin 2 expression from a pathogenic Escherichia coli strain
    • Wagner, P. L., et al. 2001. Role for a phage promoter in Shiga toxin 2 expression from a pathogenic Escherichia coli strain. J. Bacteriol. 183, 2081- 2085.
    • (2001) J. Bacteriol. , vol.183 , pp. 2081-2085
    • Wagner, P.L.1
  • 49
    • 0023780698 scopus 로고
    • Cloning and sequencing of a Shiga-like toxin type II variant from Escherichia coli strain responsible for edema disease of swine
    • Weinstein, D. L., M. P. Jackson, J. E. Samuel, R. K. Holmes, and A. D. O'Brien. 1988. Cloning and sequencing of a Shiga-like toxin type II variant from Escherichia coli strain responsible for edema disease of swine. J. Bacteriol. 170, 4223-4230.
    • (1988) J. Bacteriol. , vol.170 , pp. 4223-4230
    • Weinstein, D.L.1    Jackson, M.P.2    Samuel, J.E.3    Holmes, R.K.4    O'Brien, A.D.5
  • 50
    • 42449116191 scopus 로고    scopus 로고
    • New immuno-PCR assay for detection of low concentrations of Shiga toxin 2 and its variants
    • Zhang, W., et al. 2008. New immuno-PCR assay for detection of low concentrations of Shiga toxin 2 and its variants. J. Clin. Microbiol. 46, 1292-1297.
    • (2008) J. Clin. Microbiol. , vol.46 , pp. 1292-1297
    • Zhang, W.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.