Use of a Vero Cell-Based Fluorescent Assay to Assess Relative Toxicities of Shiga Toxin 2 Subtypes from Escherichia coli

Methods in Molecular Biology

Volumn 739, Issue , 2011, Pages 61-71

  Quiñones, Beatriz ^a   Swimley, Michelle S ^b  

^a WESTERN REGIONAL RESEARCH CENTER   (United States)

^b NONE

Author keywords

Food safety; Foodborne pathogen; Shiga toxin; Shiga toxin producing Escherichia coli; Toxin detection; Vero cells

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; VEROTOXIN 1; VEROTOXIN 2; VIRULENCE FACTOR;

ANIMAL; ARTICLE; BIOSYNTHESIS; CERCOPITHECUS; FLUORESCENCE MICROSCOPY; FOOD CONTAMINATION; ISOLATION AND PURIFICATION; METHODOLOGY; PATHOGENICITY; SHIGA TOXIN PRODUCING ESCHERICHIA COLI; TOXICITY TESTING; VERO CELL;

ANIMALS; CERCOPITHECUS AETHIOPS; FOOD CONTAMINATION; GREEN FLUORESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; SHIGA TOXIN 1; SHIGA TOXIN 2; SHIGA-TOXIGENIC ESCHERICHIA COLI; TOXICITY TESTS; VERO CELLS; VIRULENCE FACTORS;

EID: 80052524867     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-102-4_6     Document Type: Chapter

References (26)

1. Rangel JM, Sparling PH, Crowe C, Griffin PM, Swerdlow DL (2005) Epidemiology of Escherichia coli O157:H7 outbreaks, United States, 1982–2002. Emerg Infect Dis 11:603–609
2. Karmali MA (2004) Infection by Shiga toxinproducing Escherichia coli: an overview. Mol Biotechnol 26:117–122
3. Bettelheim KA (2007) The non-O157 Shigatoxigenic (verocytotoxigenic) Escherichia coli; under-rated pathogens. Crit Rev Microbiol 33:67–87
4. O’Brien AD, Kaper JD (1998) Shiga ToxinProducing Escherichia coli: yesterday, today, and tomorrow. In: Kaper JD, O’Brien AD (eds) Escherichia coli O157:H7 and other Shiga Toxin-producing E. coli strains. ASM Press, Washington, DC, pp 1–11
5. Serna A, Boedeker EC (2008) Pathogenesis and treatment of Shiga toxin-producing Escherichia coli infections. Curr Opin Gastroenterol 24:38–47
6. Boerlin P, McEwen SA, Boerlin-Petzold F, Wilson JB, Johnson RP, Gyles CL (1999) Associations between virulence factors of Shiga toxin-producing Escherichia coli and disease in humans. J Clin Microbiol 37:497–503
7. Ostroff SM, Tarr PI, Neill MA, Lewis JH, Hargrett-Bean N, Kobayashi JM (1989) Toxin genotypes and plasmid profiles as determinants of systemic sequelae in Escherichia coli O157:H7 infections. J Infect Dis 160:994–998
8. Müthing J, Schweppe CH, Karch H, Friedrich AW (2009) Shiga toxins, glycosphingolipid diversity, and endothelial cell injury. Thromb Haemost 101:252–264
9. Scheutz F, Strockbine NA (2005) Genus I. Escherichia. In: Garrity GM, Brenner DJ, Krieg NR, Staley JT (eds) Bergey’s manual of systematic bacteriology, 2nd edn. Springer, New York, pp 607–624
10. Schmidt H, Scheef J, Morabito S, Caprioli A, Wieler LH, Karch H (2000) A new Shiga toxin 2 variant (Stx2f) from Escherichia coli isolated from pigeons. Appl Environ Microbiol 66:1205–1208
11. Schmitt CK, McKee ML, O’Brien AD (1991) Two copies of Shiga-like toxin II-related genes common in enterohemorrhagic Escherichia coli strains are responsible for the antigenic heterogeneity of the O157:H-strain E32511. Infect Immun 59:1065–1073
12. Ito H, Terai A, Kurazono H, Takeda Y, Nishibuchi M (1990) Cloning and nucleotide sequencing of Vero toxin 2 variant genes from Escherichia coli O91:H21 isolated from a patient with the hemolytic uremic syndrome. Microb Pathog 8:47–60
13. 2 peptide in the context of the appropriate B pentamer. Mol Microbiol 43:207–215
14. Piérard D, Muyldermans G, Moriau L, Stevens D, Lauwers S (1998) Identification of new verocytotoxin type 2 variant B-subunit genes in human and animal Escherichia coli isolates. J Clin Microbiol 36:3317–3322
15. Persson S, Olsen KE, Ethelberg S, Scheutz F (2007) Subtyping method for Escherichia coli Shiga toxin (verocytotoxin) 2 variants and correlations to clinical manifestations. J Clin Microbiol 45:2020–2024
16. Samuel JE, Perera LP, Ward S, O’Brien AD, Ginsburg V, Krivan HC (1990) Comparison of the glycolipid receptor specificities of Shigalike toxin type II and Shiga-like toxin type II variants. Infect Immun 58:611–618
17. Waddell T, Head S, Petric M, Cohen A, Lingwood C (1988) Globotriosyl ceramide is specifically recognized by the Escherichia coli verocytotoxin 2. Biochem Biophys Res Commun 152:674–679
18. DeGrandis S, Law H, Brunton J, Gyles C, Lingwood CA (1989) Globotetraosylceramide is recognized by the pig edema disease toxin. J Biol Chem 264:12520–12525
19. Keusch GT, Jacewicz M, Acheson DWK, Donohue-Rolfe A, Kane AV, McCluer RH (1995) Globotriaosylceramide, Gb3, is an alternative functional receptor for Shiga-like toxin 2e. Infect Immun 63:1138–1141
20. Dean-Nystrom EA, Melton-Celsa AR, Pohlenz JFL, Moon HW, O’Brien AD (2003) Comparative pathogenicity of Escherichia coli O157 and intimin-negative non-O157 Shiga toxin-producing E. coli strains in neonatal pigs. Infect Immun 71:6526–6533
21. Zhang X, McDaniel AD, Wolf LE, Keusch GT, Waldor MK, Acheson DWK (2000) Quinolone antibiotics induce Shiga toxinencoding bacteriophages, toxin production, and death in mice. J Infect Dis 181:664–670
22. de Sablet T, Bertin Y, Vareille M, Girardeau JP, Garrivier A, Gobert AP, Martin C (2008) Differential expression of stx2 variants in Shiga toxin-producing Escherichia coli belonging to seropathotypes A and C. Microbiology 154:176–186
23. Zhang W, Bielaszewska M, Friedrich AW, Kuczius T, Karch H (2005) Transcriptional analysis of genes encoding Shiga toxin 2 and its variants in Escherichia coli. Appl Environ Microbiol 71:558–561
24. Quiñones B, Massey S, Friedman M, Swimley MS, Teter K (2009) Novel cell-based method to detect Shiga toxin 2 from Escherichia coli O157:H7 and inhibitors of toxin activity. Appl Environ Microbiol 75: 1410–1416
25. Cooley M, Carychao D, Crawford-Miksza L, Jay MT, Myers C, Rose C, Keys C, Farrar J, Mandrell RE (2007) Incidence and tracking of Escherichia coli O157:H7 in a major produce production region in California. PLoS ONE 2:e1159
26. Marques LRM, Peiris JSM, Cryz SJ, O’Brien AD (1987) Escherichia coli strains isolated from pigs with edema disease produce a variant of Shiga-like toxin II. FEMS Microbiol Lett 44:33–38