Low muscle glycogen concentration does not suppress the anabolic response to resistance exercise

Journal of Applied Physiology

Volumn 113, Issue 2, 2012, Pages 206-214

  Camera, Donny M ^a   West, Daniel W D ^b   Burd, Nicholas A ^b   Phillips, Stuart M ^b   Garnham, Andrew P ^c   Hawley, John A ^a   Coffey, Vernon G ^a  

^a RMIT UNIVERSITY   (Australia)

^b MCMASTER UNIVERSITY   (Canada)

^c DEAKIN UNIVERSITY   (Australia)

Author keywords

Muscle protein synthesis; Skeletal muscle

Indexed keywords

ANABOLIC AGENT; GLYCOGEN; INSULIN;

ADULT; ARTICLE; BLOOD; EXERCISE TOLERANCE; HUMAN; MALE; METABOLISM; METHODOLOGY; OXYGEN CONSUMPTION; PHYSIOLOGY; REFERENCE VALUE; RESISTANCE TRAINING; SKELETAL MUSCLE;

ANABOLIC AGENTS; EXERCISE TOLERANCE; GLYCOGEN; HUMANS; INSULIN; MALE; MUSCLE, SKELETAL; OXYGEN CONSUMPTION; REFERENCE VALUES; RESISTANCE TRAINING; YOUNG ADULT;

EID: 84864220988     PISSN: 87507587     EISSN: 15221601     Source Type: Journal    
DOI: 10.1152/japplphysiol.00395.2012     Document Type: Article

References (46)

1. Atherton PJ, Babraj JA, Smith K, Singh J, Rennie MJ, Wackerhage H. Selective activation of AMPK-PGC-1 or PKB-TSC2-mTOR signaling can explain specific adaptive responses to endurance or resistance training-like electrical muscle stimulation. FASEB J 7: 786-788, 2005.
2. Biolo G, Declan Fleming RY, Wolfe RR. Physiologic hyperinsulinemia stimulates protein synthesis and enhances transport of selected amino acids in human skeletal muscle. J Clin Invest 95: 811-819, 1995.
3. Blomstrand E, Saltin B. BCAA intake affects protein metabolism in muscle after but not during exercise in humans. Am J Physiol Endocrinol Metab 281: E365-E374, 2001.
4. Bodine SC, Latres E, Baumhueter S, Lai VKM, Nunez L, Clarke BA, Poueymirou WT, Panaro FJ, Na E, Dharmarajan K, Pan ZQ, Valenzuela DM, DeChiara TM, Stitt TN, Yancopoulos GD, Glass DJ. Identification of ubiquitin ligases required for skeletal muscle atrophy.
5. Bolster DR, Crozier SJ, Kimball SR, Jefferson LS. AMP-activated protein kinase suppresses protein synthesis in rat skeletal muscle through down-regulated mammalian target of rapamycin (mTOR) signaling. J Biol Chem 277: 23977-23980, 2002.
6. Burd NA, Tang JE, Moore DR, Phillips SM. Exercise training and protein metabolism: influences of contraction, protein intake, and sexbased differences. J Appl Physiol 106: 1692-1701, 2009.
7. Camera D, Edge J, Short M, Hawley J, Coffey V. Early time course of Akt phosphorylation after endurance and resistance exercise. Med Sci Sports Exerc 42: 1843-1852, 2010.
8. Churchley EG, Coffey VG, Pedersen DJ, Shield A, Carey KA, Cameron- Smith D, Hawley JA. Influence of preexercise muscle glycogen content on transcriptional activity of metabolic and myogenic genes in well-trained humans. J Appl Physiol 102: 1604-1611, 2007.
9. Coffey VG, Hawley JA. The molecular bases of training adaptation. Sports Med 37: 737-763, 2007.
10. Coffey VG, Pilegaard H, Garnham AP, O'Brien BJ, Hawley JA. Consecutive bouts of diverse contractile activity alter acute responses in human skeletal muscle. J Appl Physiol 106: 1187-1197, 2009.
11. Creer A, Gallagher P, Slivka D, Jemiolo B, Fink W, Trappe S. Influence of muscle glycogen availability on ERK1/2 and Akt signaling after resistance exercise in human skeletal muscle. J Appl Physiol 99: 950-956, 2005.
12. Deldicque L, De Bock K, Maris M, Ramaekers M, Nielens H, Francaux M, Hespel P. Increased p70s6k phosphorylation during intake of a protein-carbohydrate drink following resistance exercise in the fasted state. Eur J Appl Physiol 108: 791-800, 2010.
13. Dreyer HC, Drummond MJ, Pennings B, Fujita S, Glynn EL, Chinkes DL, Dhanani S, Volpi E, Rasmussen BB. Leucine-enriched essential amino acid and carbohydrate ingestion following resistance exercise enhances mTOR signaling and protein synthesis in human muscle. Am J Physiol Endocrinol Metab 294: E392-E400, 2008.
14. Dreyer HC, Fujita S, Cadenas JG, Chinkes DL, Volpi E, Rasmussen BB. Resistance exercise increases AMPK activity and reduces 4E-BP1 phosphorylation and protein synthesis in human skeletal muscle. J Physiol 576: 613-624, 2006.
15. Fujita S, Dreyer HC, Drummond MJ, Glynn EL, Cadenas JG, Yoshizawa F, Volpi E, Rasmussen BB. Nutrient signalling in the regulation of human muscle protein synthesis. J Physiol 582: 813-823, 2007.
16. Glass DJ. Skeletal muscle hypertrophy and atrophy signaling pathways. Int J Biochem Cell Biol 37: 1974-1984, 2005.
17. Glover EI, Phillips SM, Oates BR, Tang JE, Tarnopolsky MA, Selby A, Smith K, Rennie MJ. Immobilization induces anabolic resistance in human myofibrillar protein synthesis with low and high dose amino acid infusion. J Physiol 586: 6049-6061, 2008.
18. Gomes MD, Lecker SH, Jagoe RT, Navon A, Goldberg AL. Atrogin-1, a muscle-specific F-box protein highly expressed during muscle atrophy. Proc Natl Acad Sci USA 98: 14440-14445, 2001.
19. Greenhaff PL, Karagounis LG, Peirce N, Simpson EJ, Hazell M, Layfield R, Wackerhage H, Smith K, Atherton P, Selby A, Rennie MJ. Disassociation between the effects of amino acids and insulin on signaling, ubiquitin ligases, and protein turnover in human muscle. Am J Physiol Endocrinol Metab 295: E595-E604, 2008.
20. Gwinn DM, Shackelford DB, Egan DF, Mihaylova MM, Mery A, Vasquez DS, Turk BE, Shaw RJ. AMPK phosphorylation of raptor mediates a metabolic checkpoint. Mol Cell 30: 214-226, 2008.
21. Hansen AK, Fischer CP, Plomgaard P, Andersen JL, Saltin B, Pedersen BK. Skeletal muscle adaptation: training twice every second day vs. training once daily. J Appl Physiol 98: 93-99, 2005.
22. Harber MP, Crane JD, Dickinson JM, Jemiolo B, Raue U, Trappe TA, Trappe SW. Protein synthesis and the expression of growth-related genes are altered by running in human vastus lateralis and soleus muscles. Am J Physiol Regul Integr Comp Physiol 296: R708-R714, 2009.
23. Hawley JA, Burke LM, Phillips SM, Spriet LL. Nutritional modulation of training-induced skeletal muscle adaptations. J Appl Physiol 110: 834-845, 2011.
24. Hawley JA, Noakes TD. Peak power output predicts maximal oxygen uptake and performance time in trained cyclists. Eur J Appl Physiol Occup Physiol 65: 79-83, 1992.
25. Hawley JA, Tipton KD, Millard-Stafford ML. Promoting training adaptations through nutritional interventions. J Sports Sci 24: 709-721, 2006.
26. Hopkins W, Marshall S, Batterham A, Hanin J. Progressive statistics for studies in sports medicine and exercise science. Med Sci Sports Exerc 41: 3-13, 2009.
27. Howarth KR, Phillips SM, MacDonald MJ, Richards D, Moreau NA, Gibala MJ. Effect of glycogen availability on human skeletal muscle protein turnover during exercise and recovery. J Appl Physiol 109: 431-438, 2010.
28. Hulston C, Venables M, Mann C, Martin C, Philp A, Baar K, Jeukendrup A. Training with low muscle glycogen enhances fat metabolism in well-trained cyclists. Med Sci Sports Exerc 42: 2046-2055, 2010.
29. Koopman R, Beelen M, Stellingwerff T, Pennings B, Saris WHM, Kies AK, Kuipers H, van Loon LJC. Coingestion of carbohydrate with protein does not further augment postexercise muscle protein synthesis. Am J Physiol Endocrinol Metab 293: E833-E842, 2007.
30. Koopman R, Pennings B, Zorenc AHG, van Loon LJC. Protein ingestion further augments S6K1 phosphorylation in skeletal muscle following resistance type exercise in males. J Nutr 137: 1880-1886, 2007.
31. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-Δ ΔCT method. Methods 25: 402-408, 2001.
32. Louis E, Raue U, Yang Y, Jemiolo B, Trappe S. Time course of proteolytic, cytokine, and myostatin gene expression after acute exercise in human skeletal muscle. J Appl Physiol 103: 1744-1751, 2007.
33. Lowry O, Passonneau J. Some recent refinements of quantitative histochemical analysis. Curr Probl Clin Biochem 3: 63-84, 1971.
34. Moore DR, Phillips SM, Babraj JA, Smith K, Rennie MJ. Myofibrillar and collagen protein synthesis in human skeletal muscle in young men after maximal shortening and lengthening contractions. Am J Physiol Endocrinol Metab 288: E1153-E1159, 2005.
35. Moore DR, Robinson MJ, Fry JL, Tang JE, Glover EI, Wilkinson SB, Prior T, Tarnopolsky MA, Phillips SM. Ingested protein dose response of muscle and albumin protein synthesis after resistance exercise in young men. Am J Clin Nutr 89: 161-168, 2009.
36. Pasiakos SM, Vislocky LM, Carbone JW, Altieri N, Konopelski K, Freake HC, Anderson JM, Ferrando AA, Wolfe RR, Rodriguez NR. Acute energy deprivation affects skeletal muscle protein synthesis and associated intracellular signaling proteins in physically active adults. J Nutr 140: 745-751, 2010.
37. Pernow B, Saltin B. Availability of substrates and capacity for prolonged heavy exercise in man. J Appl Physiol 31: 416-422, 1971.
38. Philp A, Hamilton DL, Baar K. Signals mediating skeletal muscle remodeling by resistance exercise: PI3-kinase independent activation of mTORC1. J Appl Physiol 110: 561-568, 2011.
39. Robergs RA, Pearson DR, Costill DL, Fink WJ, Pascoe DD, Benedict MA, Lambert CP, Zachweija JJ. Muscle glycogenolysis during differing intensities of weight-resistance exercise. J Appl Physiol 70: 1700-1706, 1991.
40. Sancak Y, Peterson TR, Shaul YD, Lindquist RA, Thoreen CC, Bar-Peled L, Sabatini DM. The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Science 320: 1496-1501, 2008.
41. Thomas M, Langley B, Berry C, Sharma M, Kirk S, Bass J, Kambadur R. Myostatin, a negative regulator of muscle growth, functions by inhibiting myoblast proliferation. J Biol Chem 275: 40235-40243, 2000.
42. Thomson DM, Fick CA, Gordon SE. AMPK activation attenuates S6K1, 4E-BP1, and eEF2 signaling responses to high-frequency electrically stimulated skeletal muscle contractions. J Appl Physiol 104: 625-632, 2008.
43. West DW, Burd NA, Coffey VG, Baker SK, Burke LM, Hawley JA, Moore DR, Stellingwerff T, Phillips SM. Rapid aminoacidemia enhances myofibrillar protein synthesis and anabolic intramuscular signaling responses after resistance exercise. Am J Clin Nutr 94: 795-803, 2011.
44. Wojtaszewski JFP, MacDonald C, Nielsen JN, Hellsten Y, Hardie DG, Kemp BE, Kiens B, Richter EA. Regulation of 5=AMP-activated protein kinase activity and substrate utilization in exercising human skeletal muscle. Am J Physiol Endocrinol Metab 284: E813-E822, 2003.
45. Yeo WK, McGee SL, Carey AL, Paton CD, Garnham AP, Hargreaves M, Hawley JA. Acute signalling responses to intense endurance training commenced with low or normal muscle glycogen. Exp Physiol 95: 351-358, 2010.
46. Yeo WK, Paton CD, Garnham AP, Burke LM, Carey AL, Hawley JA. Skeletal muscle adaptation and performance responses to once a day versus twice every second day endurance training regimens. J Appl Physiol 105: 1462-1470, 2008.