Genes for Carbon metabolism and the ToxA virulence factor in pseudomonas aeruginosa are regulated through molecular interactions of PtxR and PtxS

PLoS ONE

Volumn 7, Issue 7, 2012, Pages

  Daddaoua, Abdelali ^a   Fillet, Sandy ^a   Fernández, Matilde ^b   Udaondo, Zulema ^a   Krell, Tino ^a   Ramos, Juan L ^a  

^a CSIC   (Spain)

^b Bio Iliberis R and D   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR PTXR; TRANSCRIPTION FACTOR PTXS; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; BACTERIAL VIRULENCE; BINDING AFFINITY; BINDING SITE; CARBON METABOLISM; DNA FOOTPRINTING; GENE EXPRESSION; NONHUMAN; OPERON; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; PSEUDOMONAS AERUGINOSA; TOXA GENE;

ADP RIBOSE TRANSFERASES; BACTERIAL PROTEINS; BACTERIAL TOXINS; BASE SEQUENCE; CARBON; DNA, BACTERIAL; DNA-BINDING PROTEINS; EXOTOXINS; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; GLUCONATES; OPERON; PROMOTER REGIONS, GENETIC; PROTEIN BINDING; PSEUDOMONAS AERUGINOSA; SOLUTIONS; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC; VIRULENCE FACTORS;

PSEUDOMONAS; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS SP.;

EID: 84864184091     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0039390     Document Type: Article

References (43)

1. Chastre J, Fagon JY, (2002) Ventilator-associated pneumonia. J Bacteriol 192: 4357-4366.
2. Dohar JE, Hebda PA, Veeh R, Awad M, Costerton JW, et al. (2005) Mucosal biofilm formation on middle-ear mucosa in a nonhuman primate model of chronic suppurative otitis media. Laryngoscope 8: 1469-1472.
3. Montie TC, Doyle-Huntzinger D, Craven RC, Holder IA, (1982) Loss of virulence associated with absence of flagellum in an isogenic mutant of Pseudomonas aeruginosa in the burned-mouse model. Infect Immun 3: 1296-1298.
4. Hassett DJ, Korfhagen TR, Irvin RT, Schurr MJ, Sauer K, et al. (2010) Pseudomonas aeruginosa biofilm infections in Cystic Fibrosis: insights into pathogenic processes and treatment strategies. Expert Opin. Ther Targets 14: 117-130.
5. Iglewski BH, Kabat D, (1975) NAD-dependent inhibition of protein synthesis by Pseudomonas aeruginosa toxin. Proc Natl Acad Sci USA 6: 2284-2288.
6. Hamood AN, Colmer-Hamood JA, Carty NL, (2004) Regulation of Pseudomonas aeruginosa Exotoxin A synthesis. In Pseudomonas: Virulence and Gene Regulation.Vol. 2. Edited by J.L. Ramos. Kluwer. Academic/Plenum publishers, New York. pp 389-423.
7. Frank DW, Iglewski BH, (1988) Kinetics of toxA and regA mRNA accumulation in Pseudomonas aeruginosa. J Bacteriol 10: 4477-4483.
8. Hamood AN, Colmer JA, Ochsner UA, Vasil ML, (1996) Isolation and characterization of a Pseudomonas aeruginosa gene, ptxR, which positively regulates exotoxin A production. Mol Microbiol 1: 97-110.
9. Colmer JA, Hamood AN, (1998) Characterization of ptxS, a Pseudomonas aeruginosa gene which interferes with the effect of the exotoxin A positive regulatory gene, ptxR. Mol Gen Genet 3: 250-259.
10. Gaines JM, Carty NL, Tiburzi F, Davinic M, Visca P, et al. (2007) Regulation of the Pseudomonas aeruginosa toxA, regA and ptxR genes by the iron-starvation sigma factor PvdS under reduced levels of oxygen. Microbiology 153: 4219-4233.
11. Ferrell E, Carty NL, Colmer-Hamood JA, Hamood AN, West SE, (2008) Regulation of Pseudomonas aeruginosa ptxR by Vfr. Microbiology 154: 431-439.
12. Davinic M, Carty NL, Colmer-Hamood JA, San Francisco M, Hamood AN, (2009) Role of Vfr in regulating exotoxin A production by Pseudomonas aeruginosa. Microbiology 155: 2265-2273.
13. Barton HA, Johnson Z, Cox CD, Vasil AI, Vasil ML, (1996) Ferric uptake regulator mutants of Pseudomonas aeruginosa with distinct alterations in the iron-dependent repression of exotoxin A and siderophores in aerobic and microaerobic environments. Mol Microbiol 5: 1001-1017.
14. Hedstrom RC, Funk CR, Kaper JB, Pavlovskis OR, Galloway DR, (1986) Cloning of a gene involved in regulation of exotoxin A expression in Pseudomonas aeruginosa. Infect Immun 51: 37-42.
15. Ochsner UA, Vasil AI, Vasil ML, (1995) Role of the ferric uptake regulator of Pseudomonas aeruginosa in the regulation of siderophores and exotoxin A expression: purification and activity on iron-regulated promoters. J Bacteriol 177: 7194-7201.
16. Ochsner UA, Johnson Z, Lamont IL, Cunliffe HE, Vasil ML, (1996) Exotoxin A production in Pseudomonas aeruginosa requires the iron-regulated pvdS gene encoding an alternative sigma factor. Mol Microbiol 21: 1019-1028.
17. Swanson BL, Hamood AN, (2000) Autoregulation of the Pseudomonasaeruginosa protein PtxS occurs through a specific operator site within the ptxS upstream region. J Bacteriol 182: 4366-4371.
18. Storey DG, Frank DW, Farinha MA, Kropinski AM, Iglewski BH, (1990) Multiple promoters control the regulation of the Pseudomonas aeruginosa regA gene. Mol Microbiol 4: 499-503.
19. West SE, Kaye SA, Hamood AN, Iglewski BH, (1994) Characterization of Pseudomonas aeruginosa mutants that are deficient in exotoxin A synthesis and are altered in expression of regA, a positive regulator of exotoxin A. Infect Immun 62: 897-903.
20. Colmer JA, Hamood AN, (2001) Molecular analysis of the Pseudomonas aeruginosa regulatory genes ptxR and ptxS. Can J Microbiol 47: 820-838.
21. Del Castillo T, Duque E, Ramos JL, (2008) A set of activators and repressors control peripheral glucose pathways in Pseudomonas putida to yield a common central intermediate. J Bacteriol 190: 2331-2339.
22. Del Castillo T, Ramos JL, (2007) Simultaneous catabolite repression between glucose and toluene metabolism in Pseudomonas putida is channeled through different signaling pathways. J Bacteriol 189: 6602-6610.
23. Del Castillo T, Ramos JL, Rodríguez-Herva JJ, Führer T, Sauer U, et al. (2007) Convergent peripheral pathways catalyze initial glucose catabolism in Pseudomonas putida: Genomic and flux analysis. J Bacteriol 189: 5142-5152.
24. Daddaoua A, Krell T, Alfonso C, Morel B, Ramos JL, (2010) Compartmentalized glucose metabolism in Pseudomonas putida is controlled by the PtxS repressor. J Bacteriol 192: 4357-4366.
25. Entner N, Doudoroff M, (1952) Glucose and gluconic acid oxidation of Pseudomonas saccharophila. J Biol Chem 196: 853-862.
26. Führer T, Fischer E, Sauer U, (2005) Experimental identification and quantification of glucose metabolism in seven bacterial species. J Bacteriol 187: 1581-1590.
27. Conway T, (1992) The Entner-Doudoroff pathway: history, physiology and molecular biology.FEMS Microbiol Rev 9: 1-27.
28. Sainsbury S, Lane LA, Ren J, Gilbert RJ, Saunders NJ, Robinson CV, et al. (2009) The structure of CrgA from Neisseria meningitidis reveals a new octameric assembly state for LysR transcriptional regulators. Nucleic Acids Res 37: 4545-4558.
29. Galperin MY, (2010) Diversity of structure and function of response regulator output domains. Curr Opin Microbiol 13: 150-159.
30. Buelow DR, Raivio TL, (2010) Three (and more) component regulatory systems-auxiliary regulators of bacterial histidin kinase. Mol Microbiol 75: 547-566.
31. Krell T, Lacal J, Muñoz-Martínez F, Reyes-Darias JA, et al. (2011) Diversity at its best: bacterial taxis. Env Microbiol 13: 1115-1124.
32. Scharf BE, (2010) Summary of useful methods for two-component system research. Curr Opin Microbiol 13: 246-252.
33. Thomason PA, Traynor D, Cavet G, Chang WT, Harwood AJ, et al. (1998) An intersection of the cAMP/PKA and two-component signal transduction systems in Dictyostelium. EMBO J 17: 2838-2845.
34. Ishihama A, (2010) Prokaryotic genome regulation: multifactor promoters, multitarget regulators and hierarchic networks. FEMS Microbiol Rev 34: 628-645.
35. Fillet S, Vélez M, Lu D, Zhang X, Gallegos MT, Ramos JL, (2009) TtgV represses two different promoters by recognizing different sequences. J Bacteriol 191: 1901-1909.
36. Abril MA, Michán C, Timmis KN, Ramos JL, (1989) Regulator and enzyme specificities of the TOL plasmid-encoded upper pathway for degradation of aromatic hydrocarbons and expansion of the substrate range of the pathway. J Bacteriol 171: 6782-6790.
37. Daniels C, Daddaoua A, Lu D, Zhang X, Ramos JL, (2010) Domain cross-talk during effector binding to the multidrug binding TtgR regulator. J BiolChem 285: 21372-21381.
38. Krell T, (2008) Microcalorimetry: A response to challenges in modern biotechnology. Microb Biotechnol 1: 126-136.
39. Miller JH, (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
40. Marqués S, Ramos JL, Timmis KN, (1993) Analysis of the mRNA structure of the Pseudomonas putida TOL meta fission pathway operon around the transcription initiation point, the xylTE and the xylFJ regions. Biochim Biophys Acta 1216: 227-236.
41. Sasse J, Gallagher SR, (2004) Staining proteins in gels. Curr Protoc Immunol 8: 89.
42. Hancock RE, Carey AM, (1979) Outer membrane of Pseudomonas aeruginosa: heat-2-mercaptoethanol-modifiable proteins. J Bacteriol 140: 902-910.
43. Fenner C, Traut RR, Mason DT, Wikman-Coffelt J, (1975) Quantification of Coomassie Blue stained proteins in polyacrylamide gels based on analyses of eluted dye. Anal Biochem 63: 595-602.