TtgV represses two different promoters by recognizing different sequences

Journal of Bacteriology

Volumn 191, Issue 6, 2009, Pages 1901-1909

  Fillet, Sandy ^a   Vélez, Marisela ^b   Lu, Duo ^c   Zhang, Xiaodong ^c   Gallegos, María Trinidad ^a   Ramos, Juan L ^a  

^a CSIC   (Spain)

^b Campus de Cantoblanco   (Spain)

^c IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; NUCLEOTIDE; PALINDROMIC DNA; PROTEIN TTGV; REPRESSOR PROTEIN; UNCLASSIFIED DRUG; ANTIINFECTIVE AGENT; BACTERIAL PROTEIN; CARRIER PROTEIN; TTGD PROTEIN, PSEUDOMONAS PUTIDA; TTGG PROTEIN, PSEUDOMONAS PUTIDA;

AMINO ACID SUBSTITUTION; ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; GEL MOBILITY SHIFT ASSAY; GENE EXPRESSION; GENE REPRESSION; INVERTED REPEAT; MOLECULAR RECOGNITION; MUTATIONAL ANALYSIS; NONHUMAN; OPERATOR GENE; OPERON; PRIORITY JOURNAL; PROMOTER REGION; PSEUDOMONAS PUTIDA; SEQUENCE ANALYSIS; TTGD GENE; TTGG GENE; AMINO ACID SEQUENCE; BINDING SITE; CHEMISTRY; DOWN REGULATION; DRUG EFFECT; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PROTEIN BINDING;

PSEUDOMONAS PUTIDA;

AMINO ACID SEQUENCE; ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; BASE SEQUENCE; BINDING SITES; DOWN-REGULATION; GENE EXPRESSION REGULATION, BACTERIAL; INVERTED REPEAT SEQUENCES; MEMBRANE TRANSPORT PROTEINS; MOLECULAR SEQUENCE DATA; PROMOTER REGIONS, GENETIC; PROTEIN BINDING; PSEUDOMONAS PUTIDA; REPRESSOR PROTEINS;

EID: 63049088245     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.01504-08     Document Type: Article

References (30)

1. Duque, E., A. Segura, G. Mosqueda, and J. L. Ramos. 2001. Global and cognate regulators control the expression of the organic solvent efflux pumps TtgABC and TtgDEF of Pseudomonas putida. Mol. Microbiol. 39:1100-1106.
2. Gottesman, M. M., and I. Pastam. 1993. Biochemistry of multidrug resistance mediated by the multidrug transporter. Annu. Rev. Biochem. 62:385-427.
3. Guazzaroni, M. E., T. Krell, A. Felipe, R. RuíDz, C. Meng, X. Zhang, et al. 2005. The multidrug efflux regulator TtgV recognizes a wide range of structurally different effectors in solution and complexed with target DNA. Evidence from isothermal titration calorimetry. J. Biol. Chem. 280:20887-20893.
4. Guazzaroni, M. E., T. Krell, P. Gutierrez del Arroyo, M. Vélez, M. Jiménez, G. Rivas, and J. L. Ramos. 2007. The transcriptional repressor TtgV recognizes a complex operator as a tetramer and induces convex DNA bending. J. Mol. Biol. 369:927-939.
5. Guazzaroni, M. E., W. Tera ́n, X. Zhang, M. T. Gallegos, and J. L. Ramos. 2004. TtgV bound to a complex operator site represses transcription of the single promoter for the multidrug and solvent extrusion TtgGHl pump. J. Bacteriol. 186:2921-2927.
6. Isken, S., and J. A. M. de Bont. 1996. Active efflux of toluene in a solvent-resistant bacterium. J. Bacteriol. 178:6056-6058.
7. Jerg, B., and U. Genscher. 2008. Relevance of nucleotides of the PcaV binding site from Acinetobacter baylyi. Microbiology 154:756-766.
8. Kieboom, J., J. J. Dennis, J. A. M. de Bont, and G. J. Zylstra, 1998. Identification and molecular characterization of an efflux pump involved in Pseudomonas putida S12 solvent tolerance. J. Biol. Chem. 273:85-91.
9. Kok, R G., D, A. D'Argenio, and L. N. Ornston. 1998. Mutation analysis of PobR and PcaU. closely related transcriptional activators in Acinetobacter. J. Bacteriol. 180:5058-5069.
10. Krell, T., A. J. Molina-Henares, and J. L. Ramos. 2006. The IclR family of transcriptional activators and repressors can be defined by a single profile. Protein Sci. 15:1207-1213.
11. Miller, J. H. 1972. Experiments in molecular biology. Cold Spring Harbor Laboratory Press, Cold Spring Harbor. NY.
12. Molina-Henares, A. J., T. Krell, M. E. Guazzaroni, A. Segura, and J. L. Ramos. 2006. Members of the IclR family of bacterial transcriptional regulators function as activators and/or repressors. FEMS Microbiol. Rev. 30: 157-186.
13. Mosqueda, G., and J. L. Ramos. 2000. A set of genes encoding a second toluene efflux system in Pseudomonas putida DOT-TIE is linked to the tod genes for toluene metabolism. J. Bacteriol. 182:937-943.
14. Nikaido, H. 1998. Multiple antibiotic resistance and efflux. Curr. Opin. Microbiol. 1:516-523.
15. Poole, K. 2000. Efflux-mediated resistance to fluoroquinolones in gram-positive bacteria. Antimicrob. Agents Chemother. 44:2233-2241.
16. Poole, K. 2000. Efflux-media ted resistance to fluoroquinolones in gram-positive bacteria and the mycobacteria. Antimicrob. Agents Chemother. 44:2595-2599.
17. Ramos, J. L., E. Duque, M. T. Gallegos, P. Godoy, M. I. Ramos-González, A. Rojas, W. Terán, and A. Segura. 2002. Mechanisms of solvent tolerance in gram-negative bacteria. Annu. Rev. Microbiol. 56:743-768.
18. Ramos, J. L., E. Duque, P. Godoy, and A. Segura. 1998. Efflux pumps involved in toluene tolerance in P. putida DOT-TIE. J. Bacteriol. 180:3323-3329.
19. Ramos, J. L., E. Duque, M. J. Huertas, and A. Haïdour. 1995. Isolation and expansion of the catabolic potential of a Pseudomonas putida strain able to grow in the presence of high concentrations of aromatic hydrocarbons. J. Bacteriol. 177:3911-3916.
20. Ramos, J. L., M, Martínez-Bueno, A, J, Molina-Henares, W. Terán, K. Watanabe, X. Zhang, M. T. Gallegos, R. Brennan, and R. Tobes. 2005. The TetR family of transcriptional repressors. Microbiol. Mol. Biol. Rev. 69:326-356.
21. Rhee, J. E., W. Sheng, L. K. Morgan, R. Nolet, X. Liao, and L. J. Kenney. 2008. Amino acids important for DNA recognition by the response regulator OmpR. J. Biol. Chem. 283:8664-8677.
22. Rojas, A., E. Duque, G. Mosqueda, G. Golden, A. Hurtado, J. L. Ramos, and A. Segura. 2001. Three efflux pumps are required to provide efficient tolerance to toluene in Pseudomonas putida DOT-TIE. J. Bacteriol. 183:3967-3973.
23. Rojas, A., A. Segura, M. E. Guazzaroni, W. Terán, A. Hurtado, M. T. Gallegos, and J. L. Ramos. 2003. In vivo and in vitro evidence that TtgV is the specific regulator of the TtgGHI multidrug and solvent efflux pump of Pseudomonas putida. J. Bacteriol. 185:4755-763.
24. Saier, M. J., Jr., and I. Paulsen. 2001. Phylogenv of multidrug transporters. Cell. Dev. Biol. 12:205-213.
25. Santamarta, L, M. T. Lopez-García, R. Pérez-Redondo, B. Koekman, J. F. Martin, and P. Liras. 2007. Connecting primary and secondary metabolism: AreB, an IclR-like protein, binds the ARE(ccaR) sequence of S. clavuligerus and modulates leucine biosynthesis and cephamycin C and clavulanic acid production. Mol. Microbiol. 66:511-524.
26. Spaink, H. P., J. J. H. Okker, C. A. Wijffelman, E. Pees, and B. J. J. Lugtenberg. 1987. Promoters in the nodulation region of the Rhizobium leguminosarum Sym plasmid pRL1JI. Plant Mol. Biol. 9:27-39.
27. Terán, W., A. Felipe, S. Fillet, M. E. Guazzaroni, T. Krell, R. Ruiz, J. L. Ramos, and M. T. Gallegos. 2007. Complexity in efflux pump control: cross-regulation by the paralogues TtgV and TtgT. Mol. Microbiol. 66:1416-1428.
28. Woodcock, D. M., P. J. Crowther, J. Doherty, S. Jefferson, E. DeCruz, M. Noyer-Weidner, S. S. Smith, M. Z. Michael, and M. W. Graham. 1989. Quantitative evaluation of Escherichia coli host strains for tolerance to cy-tosine methylation in plasmid and phage recombinants. Nucleic Acids Res. 17:3469-3478.
29. Yanisch-Perron, C., J. Vieira, and J. Messing. 1985. Improve M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vector. Gene 33:103-119.
30. Zhang, R. G., Y. Kim, T. Skarina, S. Beasley, R. Laskowski, C. Arrowsmith, A. Edwards, A. Joachimiak, and A. Savchenko. 2002. Crystal structure of Thermotoga maritima 0065. a member of the IclR transcriptional factor family. J. Biol. 277:19183-19190.